Basic Information | |
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Species | Cucumis sativus |
Cazyme ID | Cucsa.258820.1 |
Family | GH18 |
Protein Properties | Length: 294 Molecular Weight: 32775.2 Isoelectric Point: 8.5406 |
Chromosome | Chromosome/Scaffold: 02229 Start: 5596603 End: 5597719 |
Description | chitinase A |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH18 | 21 | 248 | 3.2e-22 |
IATYWGQNIREGRLTAACATRKFQIVNIGFLSTFGNGQPPQVNLTRHCNPICNGCWNVSAGIVNCQNDGVKVMLSIGGPHGNYSLSSAAEALDLADYIWS NFLHGHSTSPRPFGYAPLDGVDFRIERGEFSPYYTLLARRLHDYGQQWGRKVYLTAAPWCHFPDNYLTQSLHTGLFDYVWVRFFNDRQCQYNSSHPSGFR SSWMRWIHSIPAIKFYLGIPASEEAGKG |
Full Sequence |
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Protein Sequence Length: 294 Download |
MILGFVGCFF EPTTGRIHGG IATYWGQNIR EGRLTAACAT RKFQIVNIGF LSTFGNGQPP 60 QVNLTRHCNP ICNGCWNVSA GIVNCQNDGV KVMLSIGGPH GNYSLSSAAE ALDLADYIWS 120 NFLHGHSTSP RPFGYAPLDG VDFRIERGEF SPYYTLLARR LHDYGQQWGR KVYLTAAPWC 180 HFPDNYLTQS LHTGLFDYVW VRFFNDRQCQ YNSSHPSGFR SSWMRWIHSI PAIKFYLGIP 240 ASEEAGKGYV APSVLMREVL PFVKRSAGYG GVMLFDLSND VQTNYSSLIS GRV* 300 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd00598 | GH18_chitinase-like | 0.0006 | 85 | 280 | 208 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. | ||
pfam00704 | Glyco_hydro_18 | 1.0e-17 | 19 | 248 | 250 | + Glycosyl hydrolases family 18. | ||
cd02877 | GH18_hevamine_XipI_class_III | 8.0e-97 | 19 | 289 | 279 | + This conserved domain family includes xylanase inhibitor Xip-I, and the class III plant chitinases such as hevamine, concanavalin B, and PPL2, all of which have a glycosyl hydrolase family 18 (GH18) domain. Hevamine is a class III endochitinase that hydrolyzes the linear polysaccharide chains of chitin and peptidoglycan and is important for defense against pathogenic bacteria and fungi. PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2GSJ | 0 | 19 | 293 | 1 | 271 | A Chain A, Cdna Cloning And 1.75a Crystal Structure Determination Of Ppl2, A Novel Chimerolectin From Parkia Platycephala Seeds Exhibiting Endochitinolytic Activity |
GenBank | AAB34670.1 | 0 | 19 | 293 | 1 | 274 | chitinase-B, PLC-B [Phytolacca americana=pokeweed, leaves, Peptide, 274 aa] |
EMBL | CAO78600.1 | 0 | 24 | 293 | 1 | 266 | endochitinase and N-acetylglucosamine-binding hemagglutinin precursor [Parkia platycephala] |
RefSeq | XP_002276387.1 | 0 | 18 | 293 | 24 | 298 | PREDICTED: similar to chitinase [Vitis vinifera] |
RefSeq | XP_002320838.1 | 0 | 19 | 293 | 11 | 282 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2gsj_A | 0 | 19 | 293 | 1 | 271 | A Chain A, Cdna Cloning And 1.75a Crystal Structure Determination Of Ppl2, A Novel Chimerolectin From Parkia Platycephala Seeds Exhibiting Endochitinolytic Activity |
PDB | 1kr0_A | 0 | 19 | 289 | 1 | 269 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
PDB | 2hvm_A | 0 | 19 | 289 | 1 | 269 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
PDB | 1llo_A | 0 | 19 | 289 | 1 | 269 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
PDB | 1hvq_A | 0 | 19 | 289 | 1 | 269 | A Chain A, Crystal Structures Of Hevamine, A Plant Defence Protein With Chitinase And Lysozyme Activity, And Its Complex With An Inhibitor |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
JZ123100 | 272 | 19 | 289 | 0 |
CK287424 | 274 | 17 | 289 | 0 |
DT507879 | 258 | 19 | 275 | 0 |
CK287011 | 274 | 17 | 289 | 0 |
CK285812 | 274 | 17 | 289 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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