| Basic Information | |
|---|---|
| Species | Cucumis sativus |
| Cazyme ID | Cucsa.302080.1 |
| Family | GH32 |
| Protein Properties | Length: 585 Molecular Weight: 65763.3 Isoelectric Point: 6.1682 |
| Chromosome | Chromosome/Scaffold: 02951 Start: 297459 End: 301518 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 59 | 377 | 0 |
| HFQPEENWMNDPNGPLYYNGWYHFFYQYNPRAAVWGNIVWGHAVSTDLIHWLHLPLALVPDQWYDINGVWTGSATILPDGRIMMLYTGSTKEHVQVQNLA YPANLSDPLLIDWVKFSGNPVLVPPPGIDFRDFRDPTTAWFTSEGKWRIAIGSKVNRTGISLVYDTEDFKHFQLLDNLLCAVAGTGMWECLDFFPVSKDG KIGLDTSVNGPDVKHVVKTSLDDDRHDYYSLGTYDEKTATWVPDNPKIDVGIGLRYDYGLFYASKSFFDHKKGRRVLWGWIGEADSEYADVQKGWASLQG IPRTVLFDNKTGTNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 585 Download |
| MINKGLSFLS SKSPEILPPV SRGVSEGVSE KANRHFIGQN LAYFPWNNSM LSWQRTAFHF 60 QPEENWMNDP NGPLYYNGWY HFFYQYNPRA AVWGNIVWGH AVSTDLIHWL HLPLALVPDQ 120 WYDINGVWTG SATILPDGRI MMLYTGSTKE HVQVQNLAYP ANLSDPLLID WVKFSGNPVL 180 VPPPGIDFRD FRDPTTAWFT SEGKWRIAIG SKVNRTGISL VYDTEDFKHF QLLDNLLCAV 240 AGTGMWECLD FFPVSKDGKI GLDTSVNGPD VKHVVKTSLD DDRHDYYSLG TYDEKTATWV 300 PDNPKIDVGI GLRYDYGLFY ASKSFFDHKK GRRVLWGWIG EADSEYADVQ KGWASLQGIP 360 RTVLFDNKTG TNLLQWPVEE IENLRQRSHA FHNLVIHPGS VVPLEVGSST QLDIFAEFEL 420 DKEAVAKAIE TNVEFSCQRR GGAAIRGALG PFGLLVLADE TLSEHTPVYF YVAKGQNGTL 480 KTFFCTDESR SSEANDVYKP IYGSSVPVLE GEKFSLRILV DHSVVESFAQ GGRTCITSRV 540 YPTKAIYGAA RLFVFNNAKD TNITASLTIW QMNPAFIRPY HPVN* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-58 | 55 | 542 | 509 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 6.0e-81 | 55 | 543 | 500 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-97 | 65 | 380 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-149 | 59 | 377 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 59 | 532 | 484 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ04701.1 | 0 | 5 | 582 | 64 | 636 | invertase 1 [Cucumis melo] |
| DDBJ | BAB82419.1 | 0 | 10 | 580 | 75 | 644 | acid invertase [Citrus unshiu] |
| DDBJ | BAF34362.1 | 0 | 10 | 580 | 75 | 644 | beta-fructofuranosidase [Citrus sinensis] |
| RefSeq | XP_002303519.1 | 0 | 14 | 580 | 89 | 657 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 14 | 580 | 79 | 645 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 40 | 581 | 4 | 545 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
| PDB | 3ugh_A | 0 | 40 | 581 | 4 | 545 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
| PDB | 3ugg_B | 0 | 40 | 581 | 4 | 545 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
| PDB | 3ugg_A | 0 | 40 | 581 | 4 | 545 | A Chain A, Pectin Methylesterase From Yersinia Enterocolitica |
| PDB | 3ugf_B | 0 | 40 | 581 | 4 | 545 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DY919082 | 296 | 82 | 377 | 0 |
| CT842376 | 534 | 55 | 577 | 0 |
| ES821884 | 337 | 54 | 383 | 0 |
| DT550231 | 301 | 61 | 360 | 0 |
| FC906419 | 292 | 68 | 359 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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