Basic Information | |
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Species | Cucumis sativus |
Cazyme ID | Cucsa.312080.1 |
Family | PL4 |
Protein Properties | Length: 625 Molecular Weight: 70941.8 Isoelectric Point: 5.4308 |
Chromosome | Chromosome/Scaffold: 02995 Start: 1568486 End: 1574166 |
Description | Rhamnogalacturonate lyase family protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
PL4 | 1 | 604 | 0 |
MDNGIIKVNLSNPKGMVNGIEYEGIENLLSPDNDEDNRGYWDIVWNLKGSSGTKGIFDRLEATDFKVIVENEEQIELSFSRTYNNSSTGGNLIPLNIDKR FVMLRNSSGFYTYAIYEHLKEWPAFNLDNTRIAFKPRKDKFHYMAVADKRQRLMPLPDDRKPPRGQALAYPEAVLLVDPIEPDFKGEVDDKYQYGCESKD SRVHGWISTDPPIGFWQIAPTEEFRSGGPLKQFLTSHVGPTTLTVFHSTHYAGEDMVIKFGDNEPWKKVYGPIFIHLNSLPEGEDPLRLWQNAKQQMMAE VQSWPYSFIASEDFPKSDQRGTVSGKILIRERYVSDESVPASGAYVGLALPGELGSWQNESKGYQFWTRADQNGSFSLNNVRSGNYSLYGWVPNFIGNYQ YSAFIVVTPGSDIKVGELVFEPPRDGPTLWEIGIPDRTAAEFYIPDPNPKYINKLYVNHSDRFRQYGLWERYAELYPDEDLVYTVGLSDYRKDWFFSQVT RKIGDNKYAGTTWKIKFQLDSPDTNGTYRLRLALATAHGAELQVRLNDAQALPPLFTTGLIGKDNTVARHGIHGLYRLYTVDIPGGELVVGNNTILLTQA SSNS |
Full Sequence |
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Protein Sequence Length: 625 Download |
MDNGIIKVNL SNPKGMVNGI EYEGIENLLS PDNDEDNRGY WDIVWNLKGS SGTKGIFDRL 60 EATDFKVIVE NEEQIELSFS RTYNNSSTGG NLIPLNIDKR FVMLRNSSGF YTYAIYEHLK 120 EWPAFNLDNT RIAFKPRKDK FHYMAVADKR QRLMPLPDDR KPPRGQALAY PEAVLLVDPI 180 EPDFKGEVDD KYQYGCESKD SRVHGWISTD PPIGFWQIAP TEEFRSGGPL KQFLTSHVGP 240 TTLTVFHSTH YAGEDMVIKF GDNEPWKKVY GPIFIHLNSL PEGEDPLRLW QNAKQQMMAE 300 VQSWPYSFIA SEDFPKSDQR GTVSGKILIR ERYVSDESVP ASGAYVGLAL PGELGSWQNE 360 SKGYQFWTRA DQNGSFSLNN VRSGNYSLYG WVPNFIGNYQ YSAFIVVTPG SDIKVGELVF 420 EPPRDGPTLW EIGIPDRTAA EFYIPDPNPK YINKLYVNHS DRFRQYGLWE RYAELYPDED 480 LVYTVGLSDY RKDWFFSQVT RKIGDNKYAG TTWKIKFQLD SPDTNGTYRL RLALATAHGA 540 ELQVRLNDAQ ALPPLFTTGL IGKDNTVARH GIHGLYRLYT VDIPGGELVV GNNTILLTQA 600 SSNSPFVGIM YDYIRLERPL TSKM* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
pfam13620 | CarboxypepD_reg | 0.006 | 322 | 388 | 68 | + Carboxypeptidase regulatory-like domain. | ||
cd10316 | RGL4_M | 9.0e-28 | 319 | 418 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
cd10317 | RGL4_C | 4.0e-56 | 430 | 617 | 190 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
cd10320 | RGL4_N | 1.0e-68 | 1 | 279 | 282 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
pfam06045 | Rhamnogal_lyase | 6.0e-92 | 1 | 188 | 188 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_002285626.1 | 0 | 1 | 619 | 1 | 615 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002301112.1 | 0 | 1 | 617 | 1 | 612 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002527353.1 | 0 | 1 | 622 | 17 | 636 | lyase, putative [Ricinus communis] |
RefSeq | XP_002527356.1 | 0 | 1 | 619 | 130 | 746 | lyase, putative [Ricinus communis] |
RefSeq | XP_002527357.1 | 0 | 1 | 622 | 17 | 637 | lyase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3njx_A | 0.0001 | 357 | 604 | 277 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
PDB | 1nkg_A | 0.0001 | 357 | 604 | 277 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
PDB | 3njv_A | 0.0001 | 357 | 604 | 277 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
PDB | 2xhn_B | 0.0001 | 357 | 604 | 277 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
PDB | 2xhn_A | 0.0001 | 357 | 604 | 277 | 490 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
DT552229 | 294 | 1 | 293 | 0 |
DW479600 | 282 | 2 | 283 | 0 |
DW479599 | 280 | 1 | 280 | 0 |
GW864372 | 311 | 132 | 442 | 0 |
FY792769 | 270 | 321 | 590 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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