| Basic Information | |
|---|---|
| Species | Cucumis sativus |
| Cazyme ID | Cucsa.322440.1 |
| Family | GH31 |
| Protein Properties | Length: 967 Molecular Weight: 107740 Isoelectric Point: 5.124 |
| Chromosome | Chromosome/Scaffold: 03080 Start: 2177107 End: 2187351 |
| Description | heteroglycan glucosidase 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 177 | 540 | 0 |
| SSPAAALKSFSRAVGTVFMPPKWALGYHQCRWSYDSADRVLEVSRTFREKDIPCDVIWIDIDYMNGFRCFTFDPERFADPKTLADDLHQIGFKAIWMLDP GIKHEKGYFVYDSGSEKDVWVQKADGEPYVGDVWPGPCVFPEFTQAKARSWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDEEFGGCQ SHSYYHNVYGMLMARSTYEGMKLANSGRRPFVLTRAGFIGSQKYAATWTGDNSSSWDHLHMSISMTLQLGLSGQPLSGPDIGGYVGNATPRLFGRWMGIG AMFPFCRGHSEMGTADHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYIAHTTGVPVATPVFF | |||
| Full Sequence |
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| Protein Sequence Length: 967 Download |
| MANAKEEGTT KDTTISGNMI FEPILEDSVF RFDCSANDRA AAYPSFSFIK PKDRDTPISS 60 QKLPTYIPVF ECLLGQQIVK LELPAGTSLY GTGEVSGQLE RTGKRIFTWN TDAYGYGSVT 120 TSLYQSHPWV LAILPNGEAL GILADTSLRC EIDLREDSVI QFIAPSSYPV ITFGPFSSPA 180 AALKSFSRAV GTVFMPPKWA LGYHQCRWSY DSADRVLEVS RTFREKDIPC DVIWIDIDYM 240 NGFRCFTFDP ERFADPKTLA DDLHQIGFKA IWMLDPGIKH EKGYFVYDSG SEKDVWVQKA 300 DGEPYVGDVW PGPCVFPEFT QAKARSWWAN LVKDFISNGV DGIWNDMNEP AIFKTVTKTM 360 PESNIHRGDE EFGGCQSHSY YHNVYGMLMA RSTYEGMKLA NSGRRPFVLT RAGFIGSQKY 420 AATWTGDNSS SWDHLHMSIS MTLQLGLSGQ PLSGPDIGGY VGNATPRLFG RWMGIGAMFP 480 FCRGHSEMGT ADHEPWSFGE ECEEVCRLAL KRRYRLLPHI YTLFYIAHTT GVPVATPVFF 540 CSTLPNQRID NLNLTLPKGI WSRFDFGDSH PDLPVLFLQG GSIVPLGPVH QHTGEANPSD 600 DISLLVALDE NGKAEGVLFE DDGDGYGFSL GAYLLTHYVA ELESSVVTVK VSRTEGSWTR 660 PNRRLHVQIL VGGGAKIDAW GTDGELLQVT FPSEQEVADL VATSEKEYHH RLESAKTLQD 720 VEEVPEHKGV SLSKTPIELK GTCWSVKVIP WIGGRILSMT HLPSGIQWLQ GKIEINGYEE 780 YSGTEYRSAG CTEEYSIIGR NFEHEGDEES LELEGDIDGG LVLRRKIYIP KEDTKILKIN 840 SNIVAVKVGA GSGGFSRLVC LRVHPMFNLL HPTESFVSFT SIDGSVHEFW PESGEQYLEG 900 DLLPNGEWKL VDKCLGRALV NKFDIKEVHK CLIHWGTGTV NLELWSEQRP VSKETPLQIS 960 HSYEVI* 1020 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06603 | GH31_GANC_GANAB_alpha | 1.0e-128 | 191 | 531 | 345 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
| COG1501 | COG1501 | 9.0e-130 | 75 | 689 | 658 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
| pfam01055 | Glyco_hydro_31 | 6.0e-156 | 171 | 584 | 454 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| PLN02763 | PLN02763 | 0 | 15 | 966 | 975 | + hydrolase, hydrolyzing O-glycosyl compounds | ||
| cd06604 | GH31_glucosidase_II_MalA | 0 | 191 | 531 | 342 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB02784.1 | 0 | 16 | 965 | 3 | 955 | alpha glucosidase-like protein [Arabidopsis thaliana] |
| EMBL | CBI37476.1 | 0 | 16 | 965 | 81 | 1053 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002263148.1 | 0 | 19 | 965 | 1 | 969 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002326592.1 | 0 | 1 | 965 | 1 | 997 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002519886.1 | 0 | 1 | 965 | 1 | 987 | neutral alpha-glucosidase ab precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3w38_A | 0 | 46 | 623 | 162 | 798 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 3w37_A | 0 | 46 | 623 | 162 | 798 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2g3n_F | 0 | 78 | 586 | 53 | 610 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2g3n_E | 0 | 78 | 586 | 53 | 610 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2g3n_D | 0 | 78 | 586 | 53 | 610 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO779383 | 653 | 346 | 965 | 0 |
| AM723007 | 284 | 632 | 915 | 0 |
| HO783906 | 321 | 228 | 548 | 0 |
| EL478108 | 288 | 111 | 398 | 0 |
| EX405960 | 279 | 79 | 357 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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