Basic Information | |
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Species | Cucumis sativus |
Cazyme ID | Cucsa.326860.1 |
Family | GH16 |
Protein Properties | Length: 290 Molecular Weight: 32883.3 Isoelectric Point: 9.7506 |
Chromosome | Chromosome/Scaffold: 03139 Start: 2915 End: 4614 |
Description | xyloglucan endotransglucosylase/hydrolase 26 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH16 | 48 | 205 | 1.8e-28 |
QLVLDNSTGSGAKSKRSFLFGSFEARIKLVPGNSAGVVTAYYLSSTGTAHDEIDYEFLGNVTGQPYTIHTNIFTKGVGNREQQFRVWFDPTADFHNYTIH WNPNAVVWYIDGTPIRVFRNYENQGIAFPNKQGMKIYTSLWNADDWATQGGRVKTNWI |
Full Sequence |
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Protein Sequence Length: 290 Download |
MARFQSFSLA FLVIVIVSNH IAVDANISKN MVISWGHSQS KIIGDSLQLV LDNSTGSGAK 60 SKRSFLFGSF EARIKLVPGN SAGVVTAYYL SSTGTAHDEI DYEFLGNVTG QPYTIHTNIF 120 TKGVGNREQQ FRVWFDPTAD FHNYTIHWNP NAVVWYIDGT PIRVFRNYEN QGIAFPNKQG 180 MKIYTSLWNA DDWATQGGRV KTNWIYAPFI AGLRQFRPRA CYWNGVPSII QCATKSLANW 240 WNSPAYARLS RPQLAKLNEI QSKYKIYDYC KDTMRFNGKM PSECYKAQY* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd00413 | Glyco_hydrolase_16 | 3.0e-25 | 52 | 203 | 161 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-28 | 53 | 217 | 178 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
pfam00722 | Glyco_hydro_16 | 5.0e-75 | 33 | 206 | 176 | + Glycosyl hydrolases family 16. | ||
cd02176 | GH16_XET | 1.0e-135 | 22 | 284 | 266 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
PLN03161 | PLN03161 | 6.0e-162 | 1 | 289 | 291 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005618 | cell wall |
GO:0005975 | carbohydrate metabolic process |
GO:0006073 | cellular glucan metabolic process |
GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI28031.1 | 0 | 1 | 230 | 634 | 865 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_194614.1 | 0 | 1 | 289 | 1 | 292 | xyloglucan:xyloglucosyl transferase, putative / xyloglucan endotransglycosylase, putative / endo-xyloglucan transferase, putative [Arabidopsis thaliana] |
RefSeq | XP_002280688.1 | 0 | 1 | 289 | 1 | 291 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002324432.1 | 0 | 1 | 289 | 1 | 291 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002514259.1 | 0 | 1 | 289 | 1 | 291 | Xyloglucan endotransglucosylase/hydrolase protein 14 precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1un1_B | 0 | 23 | 284 | 13 | 272 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
PDB | 1un1_A | 0 | 23 | 284 | 13 | 272 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
PDB | 1umz_B | 0 | 23 | 284 | 13 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
PDB | 1umz_A | 0 | 23 | 284 | 13 | 272 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
PDB | 2uwb_B | 0 | 35 | 284 | 28 | 267 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |