| Basic Information | |
|---|---|
| Species | Cucumis sativus |
| Cazyme ID | Cucsa.360510.1 |
| Family | GH32 |
| Protein Properties | Length: 537 Molecular Weight: 60659 Isoelectric Point: 9.6609 |
| Chromosome | Chromosome/Scaffold: 03588 Start: 1147865 End: 1151051 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 11 | 330 | 0 |
| HFQPPKNWINDPNGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLA VPKNLSDPYLREWVKSPKNPLMAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDFFPVAK TGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGV QAIPRAIWLDASGKQLIQWP | |||
| Full Sequence |
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| Protein Sequence Length: 537 Download |
| HQQQPYRTSY HFQPPKNWIN DPNGPMIYKG IYHFFYQYNP KGAVWGNIVW AHSTSTDLIN 60 WEPHDHAIFP SQPSDINGCW SGSATILPGE KPAILYTGIN PKNQQVQNLA VPKNLSDPYL 120 REWVKSPKNP LMAPTPQNHI NVSSFRDPTT AWLGRDGEWR VIIGSKIDAR GLAIMYRSKD 180 FVRWNQVDHP LRYKDDMGMW ECPDFFPVAK TGRTGVEMKV SGSHVKHILK VSLDDTKHDV 240 YTVGTYNWKK DIYIPNKGSI EGYNGLRYDY GKYYASKTFF DSQKKRRVLW GWVNESSSVD 300 DDIKKGWSGV QAIPRAIWLD ASGKQLIQWP IVEIQKLRNN KVKLTNEVLK KGSTIEVKGV 360 TAAQADVEIS FKVENLKKAE ILKPEWKDPQ LLCSQKGTSS TTEGGSVGPF GLLVLASNDL 420 KEYTSVFFTV FKKPHKYVVL MCSDQSRSSL HSNNDKTTYG AFLDVDPTRE NISLRSLIDH 480 SIVESFGIKG KGCITARVYP TLAIGDNVSL YAFNNGTDSV TITKLTAWSM KKAHIN* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 4.0e-50 | 2 | 500 | 525 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-65 | 2 | 524 | 534 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 4.0e-94 | 17 | 333 | 329 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-146 | 11 | 330 | 328 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-179 | 11 | 490 | 490 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD10960.1 | 0 | 3 | 536 | 45 | 577 | cell wall invertase precursor [Fragaria x ananassa] |
| GenBank | AAT09980.1 | 0 | 3 | 536 | 46 | 576 | cell wall apoplastic invertase [Vitis vinifera] |
| EMBL | CAD91338.1 | 0 | 4 | 536 | 38 | 564 | beta-fructofuranosidase [Glycine max] |
| EMBL | CBI35961.1 | 0 | 3 | 536 | 45 | 574 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002279788.1 | 0 | 3 | 536 | 45 | 575 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 4 | 536 | 6 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 4 | 536 | 2 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 4 | 536 | 2 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 4 | 536 | 2 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 4 | 536 | 2 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CT842376 | 538 | 7 | 536 | 0 |
| DY273985 | 349 | 26 | 372 | 0 |
| AM724610 | 260 | 1 | 260 | 0 |
| FC881182 | 304 | 26 | 329 | 0 |
| JG556437 | 231 | 1 | 231 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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