Basic Information | |
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Species | Eucalyptus grandis |
Cazyme ID | Eucgr.C00881.1 |
Family | AA7 |
Protein Properties | Length: 521 Molecular Weight: 58877 Isoelectric Point: 7.4457 |
Chromosome | Chromosome/Scaffold: 3 Start: 14049917 End: 14052708 |
Description | cytokinin oxidase 3 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
AA7 | 46 | 249 | 9.2e-24 |
GNIVRETPLAVLHPSCPKDIAALVRSAYHGDGSSFAIAARGRGHSVRGQAMAPGGVVVDMTSLARHRGGGGGSAISVSWSRSLGYYADVGGGQLWRDVLE ETLWHGLSPVSWPDYLYLTVGGTLSNAGISGQTFRYGPQISNVYEMDVITGTGELTTCSPDKNPELFYSVLGGLGQFGIITRARVALDRAPTRVKWARML YSDF |
Full Sequence |
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Protein Sequence Length: 521 Download |
MVMFIITRLL LTVGKAKFPA HSLPDKLTEK LRTDPESISA ASVDFGNIVR ETPLAVLHPS 60 CPKDIAALVR SAYHGDGSSF AIAARGRGHS VRGQAMAPGG VVVDMTSLAR HRGGGGGSAI 120 SVSWSRSLGY YADVGGGQLW RDVLEETLWH GLSPVSWPDY LYLTVGGTLS NAGISGQTFR 180 YGPQISNVYE MDVITGTGEL TTCSPDKNPE LFYSVLGGLG QFGIITRARV ALDRAPTRVK 240 WARMLYSDFS DFTRDQEYLI SKNGRYQDNA LDYVEGMLLM PQGPPDSWRS SFFPLSDHPR 300 IISLLKQHKI VYCLEVAKYY DDLTQNTVNK DLEKLFEGLN HIPEFEFQKD VAYVDFLDRV 360 REGELKLTAQ GLWDVPHPWL NLFVPKSRIA DFNDGVFKNI ILKRNITTGP VLIYPMNRNK 420 WDDQMSAVVP DEDVFYTVGF LHSSGFDDWQ QFYLQNKELL SFCEDTGIEL KHYLPSFGNE 480 QEWMRHFGSK WRRFRQRKAR FDPKMIFSPG QKIFNRNHSI * |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01676 | GLDHase | 0.0005 | 167 | 319 | 155 | + galactonolactone dehydrogenase. This model represents L-Galactono-gamma-lactone dehydrogenase (EC 1.3.2.3). This enzyme catalyzes the final step in ascorbic acid biosynthesis in higher plants. This protein is homologous to ascorbic acid biosynthesis enzymes of other species: L-gulono-gamma-lactone oxidase in rat and L-galactono-gamma-lactone oxidase in yeast. All three covalently bind the cofactor FAD. | ||
pfam01565 | FAD_binding_4 | 8.0e-24 | 53 | 204 | 153 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
COG0277 | GlcD | 5.0e-29 | 29 | 511 | 491 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
pfam09265 | Cytokin-bind | 3.0e-140 | 236 | 514 | 284 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
PLN02441 | PLN02441 | 0 | 1 | 517 | 522 | + cytokinin dehydrogenase |
Gene Ontology | |
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GO Term | Description |
GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
GO:0009690 | cytokinin metabolic process |
GO:0016491 | oxidoreductase activity |
GO:0019139 | cytokinin dehydrogenase activity |
GO:0050660 | flavin adenine dinucleotide binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABN05760.1 | 0 | 1 | 517 | 13 | 540 | FAD linked oxidase, N-terminal [Medicago truncatula] |
GenBank | ACM79256.1 | 0 | 3 | 517 | 14 | 526 | cytokinin oxidase/dehydrogenase [Gossypium hirsutum] |
RefSeq | XP_002279519.1 | 0 | 1 | 515 | 13 | 519 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002308300.1 | 0 | 1 | 515 | 12 | 527 | cytokinin oxidase [Populus trichocarpa] |
RefSeq | XP_002514119.1 | 0 | 1 | 515 | 12 | 529 | gulonolactone oxidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3s1d_A | 0 | 18 | 515 | 16 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
PDB | 3s1c_A | 0 | 18 | 515 | 16 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
PDB | 3dq0_A | 0 | 18 | 515 | 16 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
PDB | 3c0p_A | 0 | 18 | 515 | 16 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
PDB | 3bw7_A | 0 | 18 | 515 | 16 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |