Basic Information | |
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Species | Eucalyptus grandis |
Cazyme ID | Eucgr.D02029.1 |
Family | GH32 |
Protein Properties | Length: 654 Molecular Weight: 72183.2 Isoelectric Point: 4.6491 |
Chromosome | Chromosome/Scaffold: 4 Start: 34442514 End: 34446090 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 132 | 447 | 0 |
HFQPEKNWMNDPNGPMYYKGWYHFFYQYNPDAAVWGNIVWGHAVSEDLIHWLYLPLAMVADQWYDINGVWSGSATVLPDGQVMMLYTGSSNESQQLQNLA YPANLSDPLLIDWVKHPGNPILAPPQCIDKLDFRDPTTAWYTSGGKWRFAVGAKINKTGVSFVYETEDFKSYKLLPGLLHAVPGTGMWECVDFFPVSDDG LDTSVNGPGVKHVMKVSLDDDRHDYYAIGTYNEEANTWVPDSPEIDVGIGIRYDYGIFYASKTFYDQNKSRRILWGWITESDSEYADVQKGWASLQALPR TIVLDTKTGSNLLQWP |
Full Sequence |
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Protein Sequence Length: 654 Download |
MAEPYPSRSP VDQEDPGYAP LAGGEEPRDH RRPIRAILAV GLSVLLVVGI VIGFSTDTSP 60 DVCAEVNSAR LEPFLLGGAG QRSVPVKTVV RPVPRGVNEG VSAKSNRVFA GANLRGFPWN 120 NSMLSWQRTA YHFQPEKNWM NDPNGPMYYK GWYHFFYQYN PDAAVWGNIV WGHAVSEDLI 180 HWLYLPLAMV ADQWYDINGV WSGSATVLPD GQVMMLYTGS SNESQQLQNL AYPANLSDPL 240 LIDWVKHPGN PILAPPQCID KLDFRDPTTA WYTSGGKWRF AVGAKINKTG VSFVYETEDF 300 KSYKLLPGLL HAVPGTGMWE CVDFFPVSDD GLDTSVNGPG VKHVMKVSLD DDRHDYYAIG 360 TYNEEANTWV PDSPEIDVGI GIRYDYGIFY ASKTFYDQNK SRRILWGWIT ESDSEYADVQ 420 KGWASLQALP RTIVLDTKTG SNLLQWPVEE VDSLRLSSKE FDSVEVEAGT VVPLDVETAT 480 QVDIVAEFEL DKQALEAAPE ADADYVCSAS GGASQRSALG PFGLLVLADD DLSEQTPVYF 540 YVVKGPNGTL KTSICTDQSR SSVATDVHKQ VYGSFVPVLE GEKLSLRILV DHSIVETFAQ 600 GGRSCITARV YPTKAIYGAA RVFLFNNATE ANVKASLKIW QMNSAFIRPY SPQ* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 7.0e-55 | 128 | 612 | 509 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 5.0e-74 | 128 | 613 | 500 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-97 | 138 | 450 | 325 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 8.0e-152 | 132 | 447 | 324 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 132 | 602 | 481 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB82419.1 | 0 | 90 | 650 | 82 | 644 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 90 | 650 | 82 | 644 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35859.1 | 0 | 64 | 652 | 62 | 651 | soluble acid invertase [Pyrus pyrifolia var. culta] |
Swiss-Prot | P29001 | 0 | 92 | 650 | 77 | 641 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
RefSeq | XP_002303519.1 | 0 | 1 | 651 | 1 | 658 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 117 | 650 | 8 | 544 | A Chain A, The X-ray Crystal Structure Of Cel9g Complexed With Cellotriose |
PDB | 3ugh_A | 0 | 117 | 650 | 8 | 544 | A Chain A, The X-ray Crystal Structure Of Cel9g Complexed With Cellotriose |
PDB | 3ugg_B | 0 | 117 | 650 | 8 | 544 | A Chain A, The X-ray Crystal Structure Of Cel9g Complexed With Cellotriose |
PDB | 3ugg_A | 0 | 117 | 650 | 8 | 544 | A Chain A, The X-ray Crystal Structure Of Cel9g Complexed With Cellotriose |
PDB | 3ugf_B | 0 | 117 | 650 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |