Basic Information | |
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Species | Eucalyptus grandis |
Cazyme ID | Eucgr.E00259.3 |
Family | GT20 |
Protein Properties | Length: 682 Molecular Weight: 77472 Isoelectric Point: 7.9881 |
Chromosome | Chromosome/Scaffold: 5 Start: 2523721 End: 2528966 |
Description | trehalose phosphatase/synthase 5 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 57 | 545 | 0 |
SQERMIIVGNQLPIRSHKRDNGEWYFSWDEDSLLYQLKDGIAQDVEVIYVGSLKEEIDMSDQDDIAQTLLDTFKCVPAFIPPDLFSKFYHGFCKQQLWPL FHYMLPLSPELGGRFDRSLWQAYLSVNKIFADKVMEVISPDDDFVWVHDYHLMVLPTFLRKRFNSVKLGFFLHSPFPSSEIYRTLPVRDDLLRALLNSDL IGFHTFDYARHFLSCCSRMLGLSYQSKRGYIGLEYYGRTVSIKILPVGIHIGQLQSVLDLPETASRVAELRDQFRGQTVMLGVDDMDIFKGISLKLLAME QLLLQHPEMRGKVVLVQIANPARGRGKDVQEVRSETFAAVKRINESFGRQGYKPIVLIDSPLQFYERIAFYVIAECCLVTAVRDGMNLIPYEYIICRQGN EKLNETLGLDQSGPRKSMLVLSEFIGCSPSLSGAIRVNPWNIDAVAEAMDYALMVSEAEKQLRHEKHYKYVSTHDVAYWARSFYQDLER |
Full Sequence |
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Protein Sequence Length: 682 Download |
MVSRSYSNLL DLTSGEALGF GRERRRFSRV ATVSGPLSEL DDDNSNSVGS DAPSSLSQER 60 MIIVGNQLPI RSHKRDNGEW YFSWDEDSLL YQLKDGIAQD VEVIYVGSLK EEIDMSDQDD 120 IAQTLLDTFK CVPAFIPPDL FSKFYHGFCK QQLWPLFHYM LPLSPELGGR FDRSLWQAYL 180 SVNKIFADKV MEVISPDDDF VWVHDYHLMV LPTFLRKRFN SVKLGFFLHS PFPSSEIYRT 240 LPVRDDLLRA LLNSDLIGFH TFDYARHFLS CCSRMLGLSY QSKRGYIGLE YYGRTVSIKI 300 LPVGIHIGQL QSVLDLPETA SRVAELRDQF RGQTVMLGVD DMDIFKGISL KLLAMEQLLL 360 QHPEMRGKVV LVQIANPARG RGKDVQEVRS ETFAAVKRIN ESFGRQGYKP IVLIDSPLQF 420 YERIAFYVIA ECCLVTAVRD GMNLIPYEYI ICRQGNEKLN ETLGLDQSGP RKSMLVLSEF 480 IGCSPSLSGA IRVNPWNIDA VAEAMDYALM VSEAEKQLRH EKHYKYVSTH DVAYWARSFY 540 QDLERACRDH VRRRCWGIGF GLGFRVIALD PSFKKLSVDH IVSAYKRTKS RAILLDYDGA 600 MGSTGSNSIS VIPTAETVGL INSLCRDPKN VVFLVSGKER VILSKWFSSC DRLGLAAEHG 660 YFLRRAKPRR RLGNLCFGDR F* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR02400 | trehalose_OtsA | 2.0e-141 | 60 | 545 | 489 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
PRK14501 | PRK14501 | 9.0e-142 | 60 | 664 | 607 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
PLN02205 | PLN02205 | 0 | 1 | 664 | 670 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
pfam00982 | Glyco_transf_20 | 0 | 59 | 545 | 491 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
cd03788 | GT1_TPS | 0 | 60 | 544 | 485 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABD65165.1 | 0 | 1 | 664 | 1 | 663 | trehalose-6-phosphate synthase, putative [Brassica oleracea] |
EMBL | CAN61971.1 | 0 | 1 | 664 | 1 | 664 | hypothetical protein [Vitis vinifera] |
RefSeq | NP_567538.1 | 0 | 1 | 664 | 1 | 663 | ATTPS5; protein binding / transferase, transferring glycosyl groups / trehalose-phosphatase [Arabidopsis thaliana] |
RefSeq | XP_002304347.1 | 0 | 1 | 664 | 1 | 662 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002326738.1 | 0 | 1 | 663 | 1 | 662 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1uqu_B | 0 | 141 | 545 | 73 | 453 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1uqu_A | 0 | 141 | 545 | 73 | 453 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1uqt_B | 0 | 141 | 545 | 73 | 453 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1uqt_A | 0 | 141 | 545 | 73 | 453 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1gz5_D | 0 | 141 | 545 | 72 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO777775 | 588 | 80 | 664 | 0 |
HO779327 | 509 | 98 | 606 | 0 |
DT564774 | 295 | 111 | 405 | 0 |
HO779327 | 55 | 613 | 667 | 0.00000000002 |
HO777775 | 81 | 1 | 80 | 0.000004 |
Sequence Alignments (This image is cropped. Click for full image.) |
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