Basic Information | |
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Species | Eucalyptus grandis |
Cazyme ID | Eucgr.F01770.1 |
Family | GH13 |
Protein Properties | Length: 846 Molecular Weight: 96544.8 Isoelectric Point: 4.9842 |
Chromosome | Chromosome/Scaffold: 6 Start: 22983878 End: 22993329 |
Description | starch branching enzyme 2.1 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 300 | 617 | 1.7e-28 |
YNTVQLMAVMEHSYYGSFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLRVLMDVVHSHASNNVTDGLNGFDIGQSSQESYFHTGDRGYHKLWDSRLFN YANWEVLRFLLSNLRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNYGEYFSEATDVDAVVYLMLANCLIHNILPDATVIAEDVSGMPGLGRPVSEGG VGFDYRLAMAIPDRWIDYLKNKKDEEWSMKEISWSLTNRRYSEKCISYAESHDQAIVGDKTIAFLLMDKEMYSGMSCLTDASPSVERGLALHKMIHFITM ALGGEGYLNFMGNEFGHP |
Full Sequence |
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Protein Sequence Length: 846 Download |
MYSSCGFASV PPAVGSSSPS SRTQCAQTSR NSRTVANRQF GGVFSQSSQL FRRHKLHFPS 60 RVQRQGRADR NPKILGVLTD DSSTMTPIED EIERIGILNV DPGLEPFRDH FKYRVKRYVD 120 QKKLFEKHEV GLEEFAKGYL TFGFNREDGD IVYREWAPAA QEAQLVGDFN GWDGSNHNME 180 KNQFGVWTIK VPDINGVPAI PHNSRVKFRF KPGNGVWIDR IPAWIKYAIV EPSSFAAPYD 240 GVYWDPPPSE RYQFKYPRPP KPDAPRIYEA HVGMSSSEPR VNSYREFADD VLPRIWANNY 300 NTVQLMAVME HSYYGSFGYH VTNFFAVSSR SGTPEDLKYL IDKAHSLGLR VLMDVVHSHA 360 SNNVTDGLNG FDIGQSSQES YFHTGDRGYH KLWDSRLFNY ANWEVLRFLL SNLRWWLEEY 420 QFDGFRFDGV TSMLYHHHGI NMAFTGNYGE YFSEATDVDA VVYLMLANCL IHNILPDATV 480 IAEDVSGMPG LGRPVSEGGV GFDYRLAMAI PDRWIDYLKN KKDEEWSMKE ISWSLTNRRY 540 SEKCISYAES HDQAIVGDKT IAFLLMDKEM YSGMSCLTDA SPSVERGLAL HKMIHFITMA 600 LGGEGYLNFM GNEFGHPDWI DFPREGNNWS YDKCRRQWNL VDAEHLRYKF MNAFDRAMNL 660 LDEKFSILAS TKQIVSSTNE EDKVIVFEHG DLVFVFNFHP ENTYDGYKVG CDLPGKYRVA 720 LDSDAWDFGG HGRVGHGFDH FTCPEGIPGV PETNFNNRPN SFKVLSPART CVAYYRVDES 780 LEDIDLQEND PDTVEEVDDS VMTDVVARQA DLEESMLRLE EKDDDIDPKA MRDYEVDFGE 840 GTSDD* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 2.0e-8 | 45 | 193 | 157 | + alpha-amylase | ||
PLN03244 | PLN03244 | 2.0e-141 | 197 | 737 | 545 | + alpha-amylase; Provisional | ||
PLN02447 | PLN02447 | 0 | 79 | 799 | 723 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 248 | 656 | 409 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02960 | PLN02960 | 0 | 197 | 775 | 585 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABN05321.1 | 0 | 47 | 815 | 45 | 807 | starch branching enzyme I [Populus trichocarpa] |
EMBL | CAA54308.1 | 0 | 1 | 830 | 1 | 837 | 1,4-alpha-glucan branching enzyme [Manihot esculenta] |
EMBL | CBI18866.1 | 0 | 1 | 845 | 1 | 840 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002284841.1 | 0 | 49 | 845 | 28 | 817 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002510672.1 | 0 | 42 | 814 | 85 | 871 | starch branching enzyme II, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3aml_A | 0 | 85 | 784 | 1 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3amk_A | 0 | 85 | 784 | 1 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 85 | 784 | 1 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 85 | 784 | 1 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 1m7x_D | 9.94922e-44 | 156 | 730 | 32 | 577 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO619167 | 601 | 181 | 780 | 0 |
HO794536 | 714 | 97 | 798 | 0 |
HO777638 | 660 | 151 | 798 | 0 |
CX109187 | 391 | 394 | 784 | 0 |
HO777638 | 47 | 100 | 146 | 6 |
Sequence Alignments (This image is cropped. Click for full image.) |
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