| Basic Information | |
|---|---|
| Species | Eucalyptus grandis |
| Cazyme ID | Eucgr.I02168.1 |
| Family | GT68 |
| Protein Properties | Length: 584 Molecular Weight: 65567.5 Isoelectric Point: 6.4668 |
| Chromosome | Chromosome/Scaffold: 9 Start: 31541206 End: 31544952 |
| Description | O-fucosyltransferase family protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GT68 | 265 | 558 | 0 |
| GRILVVPSSKVDYEYNKVLDVDHINDCLGRKVVMSFEEFSGVKKTHLHIDRFICYFSSPQHCYVDDEHVKKLKGLGISMGKLESPWVEDTQKPNKRTIQD VEAKFTVDDDVIAIGDVFFADLEQEWVTQPGGPLAHKCKALIEPSHLIVLTAQRFIQTFLGKNYIALHFRRHGFLKFCNAKTPSCFYPVPQAAECIKRVV ERTDAPVIYLSTDAAESETGLLQTLIFSNGKPIPLVKRPDRSPAEKWDALLYRHGLQDDSQVEAMLDKTICAMSRVFIGASGSTFTEDILRLRK | |||
| Full Sequence |
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| Protein Sequence Length: 584 Download |
| MERDWSDEDD DSHHLIHQND TKPVSSSTSR RGRSAFDVED LDSRIGAARR FPFRFNKSYL 60 LAILISVLFL VAFFSADIRG LFSGHASAFG LGSTADRMRE SELRALSLLK QQRLGLFGLW 120 NHTFLDSAIA RNGTANFTLS SSNSSVLKKR DSGHLLASSS ALFEDFKSAV LRQIKLNKDI 180 EEVLLLPHQS GNVSVENGDG DMVDVNFSDF GFDQCRTVDQ NSSGRKTIEW KPKPDKFLLA 240 ICVSGQMSNH LICLEKHMFF AAVLGRILVV PSSKVDYEYN KVLDVDHIND CLGRKVVMSF 300 EEFSGVKKTH LHIDRFICYF SSPQHCYVDD EHVKKLKGLG ISMGKLESPW VEDTQKPNKR 360 TIQDVEAKFT VDDDVIAIGD VFFADLEQEW VTQPGGPLAH KCKALIEPSH LIVLTAQRFI 420 QTFLGKNYIA LHFRRHGFLK FCNAKTPSCF YPVPQAAECI KRVVERTDAP VIYLSTDAAE 480 SETGLLQTLI FSNGKPIPLV KRPDRSPAEK WDALLYRHGL QDDSQVEAML DKTICAMSRV 540 FIGASGSTFT EDILRLRKDW GSASRCDEYL CQGEEPNFIA DDE* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd11302 | O-FucT-1 | 0.007 | 406 | 558 | 179 | + GDP-fucose protein O-fucosyltransferase 1. The protein O-fucosyltransferase 1 (Ofut1 or O-FucT-1) adds O-fucose to EGF (epidermal growth factor-like) repeats. The O-fucsosylation of the Notch receptor signaling protein is dependent on this enzyme, which requires GDP-fucose as a substrate. O-fucose residues added to the target of O-FucT-1 may be further elongated by other glycosyltransferases. On top of O-fucosylation, O-FucT-1 may have other functions such as the regulation of the Notch receptor exit from the ER. Six highly conserved cysteines are present in O-FucT-1, which is a soluble ER protein, as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The membrane-bound pre-protein is released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. O-FucT-1 is similar to family 1 glycosyltransferases (GT1). | ||
| cd11296 | O-FucT_like | 0.0003 | 237 | 278 | 42 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| pfam10250 | O-FucT | 7.0e-5 | 260 | 550 | 326 | + GDP-fucose protein O-fucosyltransferase. This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present in O-FucT-1 as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. | ||
| cd11298 | O-FucT-2 | 3.0e-10 | 424 | 550 | 129 | + GDP-fucose protein O-fucosyltransferase 2. O-FucT-2 adds O-fucose to thrombospondin type 1 repeats (TSRs), and appears conserved in bilateria. The O-fucosylation of TSRs appears to play a role in regulating secretion of metalloproteases of the ADAMTS superfamily. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| cd11296 | O-FucT_like | 1.0e-17 | 398 | 557 | 170 | + GDP-fucose protein O-fucosyltransferase and related proteins. O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM66093.1 | 0 | 1 | 583 | 1 | 566 | unknown [Arabidopsis thaliana] |
| RefSeq | NP_173170.2 | 0 | 1 | 583 | 1 | 564 | unknown protein [Arabidopsis thaliana] |
| RefSeq | NP_199853.1 | 0 | 1 | 583 | 1 | 566 | unknown protein [Arabidopsis thaliana] |
| RefSeq | XP_002264087.1 | 0 | 1 | 583 | 1 | 559 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002303337.1 | 0 | 1 | 583 | 1 | 527 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4ap6_D | 0.001 | 424 | 550 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_C | 0.001 | 424 | 550 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_B | 0.001 | 424 | 550 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap6_A | 0.001 | 424 | 550 | 277 | 383 | A Chain A, Crystal Structure Of Human Pofut2 E54a Mutant In Complex With Gdp- Fucose |
| PDB | 4ap5_B | 0.002 | 424 | 550 | 263 | 369 | A Chain A, Crystal Structure Of Human Pofut2 |
