| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G001304_T01 |
| Family | GT20 |
| Protein Properties | Length: 577 Molecular Weight: 64563.7 Isoelectric Point: 6.1543 |
| Chromosome | Chromosome/Scaffold: 6 Start: 159251140 End: 159256319 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GT20 | 1 | 195 | 0 |
| MLGVDRLDMIKGIPQKILAFEKFLEENPDWNDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALV TSLRDGMNLVSYEYVACQGSKKGVLILSEFAGAAQSLGAGAILVNPWNITEVADSIHHALTMPSDEREKRHRHNYAHVTTHTAQDWAETFVFELN | |||
| Full Sequence |
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| Protein Sequence Length: 577 Download |
| MLGVDRLDMI KGIPQKILAF EKFLEENPDW NDKVVLLQIA VPTRTDVPEY QKLTSQVHEI 60 VGRINGRFGT LTAVPIHHLD RSLDFHALCA LYAVTDVALV TSLRDGMNLV SYEYVACQGS 120 KKGVLILSEF AGAAQSLGAG AILVNPWNIT EVADSIHHAL TMPSDEREKR HRHNYAHVTT 180 HTAQDWAETF VFELNDTVAE ALLRTRQVPP GLPGQTAIQQ YLRSKNRLLI LGFNSTLTEP 240 VESSGRRGGD QIKEMELKLH PDLKGPLGAL CEDERTTVIV LSGSDRSVLD ENFGEFKMWL 300 AAEHGMFLRP TYGEWMTTMP EHLNMDWVDS VKHVFEYFTE RTPRSHFEHR ETSFVWNYKY 360 ADVEFGRLQA RDMLQHLWTG PISNAAVDVV QGSRSVEVRS VGVTKGAAID RILGEIVHSE 420 NMITPIDYVL CIGHFLGKDE DIYVFFDPEY PSESKVKPEG GSASLDRRPN GRPASNGRSN 480 SRNPQSRPQK AQQAASERSS SSSHSSTSSN HDWREGSSVL DLKAENYFSC AVGRKRSNAR 540 YLLSSSEEVV SFLKELATET AGFQSSCADY MFLDRQ* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR02400 | trehalose_OtsA | 8.0e-113 | 1 | 196 | 196 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 5.0e-113 | 1 | 196 | 196 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 5.0e-131 | 2 | 433 | 432 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| PLN03064 | PLN03064 | 0 | 1 | 564 | 571 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 1 | 558 | 558 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACE79166.1 | 0 | 24 | 576 | 5 | 557 | trehalose-6-phosphate synthase 1 [Saccharum spp. complex hybrid] |
| GenBank | ACI16353.1 | 0 | 1 | 576 | 275 | 859 | trehalose-6-P synthase [Triticum aestivum] |
| GenBank | EAY98715.1 | 0 | 1 | 576 | 275 | 855 | hypothetical protein OsI_20647 [Oryza sativa Indica Group] |
| RefSeq | NP_001123593.2 | 0 | 1 | 571 | 365 | 935 | trehalose-6-phosphate synthase [Zea mays] |
| RefSeq | XP_002440086.1 | 0 | 1 | 576 | 400 | 977 | hypothetical protein SORBIDRAFT_09g025790 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 7e-40 | 1 | 198 | 258 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 7e-40 | 1 | 198 | 258 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 7e-40 | 1 | 198 | 258 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 7e-40 | 1 | 198 | 258 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 8e-40 | 1 | 198 | 257 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Hydropathy |
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