| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G009282_T01 |
| Family | GH31 |
| Protein Properties | Length: 928 Molecular Weight: 102342 Isoelectric Point: 7.251 |
| Chromosome | Chromosome/Scaffold: 3 Start: 22814532 End: 22820273 |
| Description | alpha-xylosidase 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 268 | 761 | 0 |
| FYFFSGPTPLAIVDQYTSMIGRPAPMPYWAFGFHQCRWGYKNLSVVEGVVEGYRNAQIPLDVIWNDDDHMDAAKDFTLDPVNYPRPKLLAFLDKIHAQGM KYIVLIDPGIAVNSSYGVYQRGMERDIFIKLDGQPYLAQVWPGPVYFPDFLNPNGVSWWIDEVRRFHDLVPVDGLWIDMNEASNFCTGKCTIPKTHQCPI PDTKTPWLCCLDCKNLTNTRWDEPPYKINASGQTARLGFNTIATSATHYNGILEYNAHSLYGFSQAIATHTALQGLQGKRPFILTRSTFVGSGAYAAHWT GDNKGTWENLRYSISTMLNFGIFGMPMVGSDICGFYPSPTEELCNRWIELGAFYPFSRDHANFASPRQELYVWDSVAKSARNALGMRYKLLPYLYTLNYQ AHLTGAPVARPVFFSFPDFTPCYGLSTQFLLGPGVMVSPVLEQGATSVSAMFPPGTWYNLFDMSKVVVSRSGAPVKLDAPLNEINVHVYQNTIL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 928 Download |
| MLSFSRSAIL SYLLWCLLFL TLASSNGAVA TAKPKAGCGY KLVSLVQLPN GGGLVGYLQV 60 KQRTSTYGPD IPRLRLFVKH ETRERVRVQI TDADKQRWEV PYNLLPREPA PPVTGGKVTG 120 APFTGAEYPG EELVFTYGRD PFWFAVHRRS TRQPLFNTSA GALVFKDQYL EVSTALPKNA 180 ALYGLGENTQ PGGIRLRPND PYTIYTTDIS AINLNTDLYG SHPVYMDLRS LGGRGVAHAV 240 LLLNSNGMDV FYRGTSLTYK VIGGLLDFYF FSGPTPLAIV DQYTSMIGRP APMPYWAFGF 300 HQCRWGYKNL SVVEGVVEGY RNAQIPLDVI WNDDDHMDAA KDFTLDPVNY PRPKLLAFLD 360 KIHAQGMKYI VLIDPGIAVN SSYGVYQRGM ERDIFIKLDG QPYLAQVWPG PVYFPDFLNP 420 NGVSWWIDEV RRFHDLVPVD GLWIDMNEAS NFCTGKCTIP KTHQCPIPDT KTPWLCCLDC 480 KNLTNTRWDE PPYKINASGQ TARLGFNTIA TSATHYNGIL EYNAHSLYGF SQAIATHTAL 540 QGLQGKRPFI LTRSTFVGSG AYAAHWTGDN KGTWENLRYS ISTMLNFGIF GMPMVGSDIC 600 GFYPSPTEEL CNRWIELGAF YPFSRDHANF ASPRQELYVW DSVAKSARNA LGMRYKLLPY 660 LYTLNYQAHL TGAPVARPVF FSFPDFTPCY GLSTQFLLGP GVMVSPVLEQ GATSVSAMFP 720 PGTWYNLFDM SKVVVSRSGA PVKLDAPLNE INVHVYQNTI LPMQRGGFVS KDARATPFTL 780 VVAFPFGATQ ADAEGAVYVD DDERPEMVLA EGQASYVRFH ASVRGKAVTV RSEVLMGSYS 840 LHKGLVVEKL SVLGLEGTGK DLAIQVDGTD ATAVATSSPY FAAGGNAKLQ GEEGVEDSKN 900 GVMVEIGGLA LPLGKSFTMT WNMRIEA* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01055 | Glyco_hydro_31 | 4.0e-86 | 269 | 456 | 188 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| cd06602 | GH31_MGAM_SI_GAA | 5.0e-94 | 522 | 685 | 165 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). | ||
| cd06603 | GH31_GANC_GANAB_alpha | 4.0e-100 | 288 | 672 | 391 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
| pfam01055 | Glyco_hydro_31 | 1.0e-109 | 514 | 761 | 252 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| cd06604 | GH31_glucosidase_II_MalA | 9.0e-116 | 288 | 672 | 391 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAA99366.1 | 0 | 16 | 927 | 19 | 929 | putative alpha-glucosidase [Oryza sativa Japonica Group] |
| DDBJ | BAG72143.1 | 0 | 23 | 927 | 26 | 954 | alpha-glucosidase [Hordeum vulgare subsp. vulgare] |
| GenBank | EEC69878.1 | 0 | 16 | 927 | 19 | 929 | hypothetical protein OsI_00249 [Oryza sativa Indica Group] |
| RefSeq | NP_001041922.1 | 0 | 16 | 927 | 22 | 932 | Os01g0130400 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002457434.1 | 0 | 1 | 927 | 1 | 928 | hypothetical protein SORBIDRAFT_03g007230 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3w38_A | 0 | 8 | 856 | 12 | 852 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 3w37_A | 0 | 8 | 856 | 12 | 852 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 3lpp_D | 0 | 67 | 921 | 106 | 898 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 3lpp_C | 0 | 67 | 921 | 106 | 898 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 3lpp_B | 0 | 67 | 921 | 106 | 898 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
