| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G018692_T01 |
| Family | GH32 |
| Protein Properties | Length: 604 Molecular Weight: 65435.5 Isoelectric Point: 5.344 |
| Chromosome | Chromosome/Scaffold: 2 Start: 3229044 End: 3231455 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 54 | 372 | 0 |
| HFQPAKNWQNGPVYYNGMYHLFYQYNPHGALWDVGNLSWGDSVSGDLVNWAALGNALDPTAPFDANGCASGSVTILPDGTPAILYSGIDADRRQVQNVAF PKNPRDPLLREWAKPAYNPVVPLPADVSANDFRDPTTAWVGRDGLWRFAISAVADGVGATLVYRSADFLRWERRAAPLHASQDAVMAECPDLFPVAARGA EGLDASARGAGVRHVLKVSMPDTLEDYYAVGTYDDAADTFTPDEDCGGGDYRRWRRIDRGHLYASKTFLDARRMRRVLWAWVNESDSEADDVARGWSGLQ SFPRALWLDGGGKQLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 604 Download |
| MCHSHSLVTG PMAANPLATL AIAVFLICLL LPTSSSSSSI CVARTQDHVR TAFHFQPAKN 60 WQNGPVYYNG MYHLFYQYNP HGALWDVGNL SWGDSVSGDL VNWAALGNAL DPTAPFDANG 120 CASGSVTILP DGTPAILYSG IDADRRQVQN VAFPKNPRDP LLREWAKPAY NPVVPLPADV 180 SANDFRDPTT AWVGRDGLWR FAISAVADGV GATLVYRSAD FLRWERRAAP LHASQDAVMA 240 ECPDLFPVAA RGAEGLDASA RGAGVRHVLK VSMPDTLEDY YAVGTYDDAA DTFTPDEDCG 300 GGDYRRWRRI DRGHLYASKT FLDARRMRRV LWAWVNESDS EADDVARGWS GLQSFPRALW 360 LDGGGKQLVQ WPVEEIETLR TRRAAPLQEV EPGGGVREVT GIVSSQADVD VVFEIPSLRR 420 AEGLDPGRLH DPDALCREKG GSLTGGVGPF GLLVMASGDM REHTAVFFRV FRILHEYAVL 480 MCTDLSRSST KAGVYKPTHG GFVDVDIEKD MSISLRTLID HSIVESFGGG GRTCMTARVY 540 PEHVAAGSSS HLYVFNNGSA AVKVSKLEAW ELASASVNVD DDGGLMAGGS FANMCRAEEY 600 NSS* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 7.0e-41 | 46 | 381 | 369 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 8.0e-55 | 45 | 551 | 525 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 5.0e-79 | 63 | 375 | 325 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 4.0e-123 | 54 | 372 | 330 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 5.0e-152 | 54 | 531 | 491 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE03580.1 | 0 | 12 | 589 | 3 | 585 | OSJNBa0087O24.3 [Oryza sativa (japonica cultivar-group)] |
| EMBL | CAH65975.1 | 0 | 12 | 589 | 3 | 585 | H1005F08.4 [Oryza sativa (indica cultivar-group)] |
| RefSeq | NP_001054172.1 | 0 | 12 | 595 | 3 | 591 | Os04g0664800 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | Q56UD0 | 0 | 12 | 589 | 3 | 588 | INV6_ORYSJ RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 6; AltName: Full=Sucrose hydrolase 6; AltName: Full=Invertase 6; AltName: Full=Cell wall beta-fructosidase 6; AltName: Full=OsCIN6; Flags: Precursor |
| RefSeq | XP_002448711.1 | 0 | 32 | 600 | 21 | 587 | hypothetical protein SORBIDRAFT_06g031910 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 50 | 578 | 9 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 50 | 578 | 5 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 50 | 578 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 50 | 578 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 50 | 578 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
