| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G018716_T01 |
| Family | GH32 |
| Protein Properties | Length: 553 Molecular Weight: 60462.6 Isoelectric Point: 5.955 |
| Chromosome | Chromosome/Scaffold: 2 Start: 3224667 End: 3227301 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 15 | 329 | 0 |
| HFQPAKNWMNDPNGPLYHKGMYHMFFQYNPHGPTFGTGKLSWGHSVSGDLVNWAFLGTALDPTSPFDAEGCWSGSATTLADGRPAILYTGRDASGVQVQN VAFPKNPSDPLLREWRKPPGCNPVVPQPGDVTGNNFRDPTTAWLGRDGLWRFAVAAEVGGVGSTLVYRSKDFVRWERGSAPLHASPDVPVWECPDLFPVA ERGAEGLDTSARGGAGVRHVLKLSKAADEDYYVVGRYDDEADTFAPVEGVFDWRRIDHGHLFGAKTFFDARRRRRVLWAWVDETDDGVDKGWRGIQTFPR ALWLDADGRQLVQWP | |||
| Full Sequence |
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| Protein Sequence Length: 553 Download |
| MNGKQSPRNG RTAYHFQPAK NWMNDPNGPL YHKGMYHMFF QYNPHGPTFG TGKLSWGHSV 60 SGDLVNWAFL GTALDPTSPF DAEGCWSGSA TTLADGRPAI LYTGRDASGV QVQNVAFPKN 120 PSDPLLREWR KPPGCNPVVP QPGDVTGNNF RDPTTAWLGR DGLWRFAVAA EVGGVGSTLV 180 YRSKDFVRWE RGSAPLHASP DVPVWECPDL FPVAERGAEG LDTSARGGAG VRHVLKLSKA 240 ADEDYYVVGR YDDEADTFAP VEGVFDWRRI DHGHLFGAKT FFDARRRRRV LWAWVDETDD 300 GVDKGWRGIQ TFPRALWLDA DGRQLVQWPV EEIETLRKGR AALAGAVVGA GGLREIAGVD 360 ALQADVEVVF EVPESLDDAE ELDPEWLQHD PHKLRACAEK KASSPGPRGG VGPFGLVVMA 420 SGDMREQTTV FFQVLRHGGT YKVLMCADLT RSSTKEGVHK PFYAGFVDVD VEKDRGISLR 480 TLIDHSVVES FGGGGRTCIT ARVYPEHVAA GGSSHLYLFN NGAHPVTVSK IEAWELGTAS 540 VNVEQDDRAD PL* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 7.0e-56 | 11 | 338 | 346 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 8.0e-85 | 21 | 332 | 321 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-116 | 15 | 329 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-134 | 15 | 495 | 497 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACG30063.1 | 0 | 1 | 552 | 1 | 552 | beta-fructofuranosidase, insoluble isoenzyme 7 precursor [Zea mays] |
| EMBL | CAH65976.1 | 0 | 1 | 542 | 4 | 544 | H1005F08.5 [Oryza sativa (indica cultivar-group)] |
| GenBank | EEC78196.1 | 0 | 1 | 542 | 4 | 547 | hypothetical protein OsI_17809 [Oryza sativa Indica Group] |
| RefSeq | NP_001145776.1 | 0 | 1 | 552 | 1 | 552 | hypothetical protein LOC100279283 [Zea mays] |
| RefSeq | XP_002448712.1 | 0 | 1 | 552 | 1 | 556 | hypothetical protein SORBIDRAFT_06g031920 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 11 | 549 | 8 | 541 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 2ade_A | 0 | 11 | 549 | 8 | 541 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 2add_A | 0 | 11 | 549 | 8 | 541 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 1st8_A | 0 | 11 | 549 | 8 | 541 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 11 | 549 | 8 | 541 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Hydropathy |
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