| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G139300_T01 |
| Family | GH32 |
| Protein Properties | Length: 612 Molecular Weight: 67442.7 Isoelectric Point: 7.9046 |
| Chromosome | Chromosome/Scaffold: 5 Start: 169454598 End: 169459090 |
| Description | cell wall invertase 2 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 79 | 398 | 0 |
| HFQPPMNWINDPNAPLYYKGWYHLFYQYNPKGAVWGNIVWAHSVSRDLINWVALEPAIYPSIPSDKYGCWSGSATILEDGTPAILYTGIDRADINYQVQV LALPKDASDPLLREWEKPEEYNPVATPAAGGINATQFRDPTTAWRHAGHWRMLVGSVRGARGMALVYRSRDFRKWTKAKHPLHSAALTGMWECPDFFPVS GPGLQAGLDTSAPGRKYVLKSSLDLTRYDYYTIGSYDGGKDRYYPDDPAGDYHHRLRYDYGNYYASKTFYDPVERRRVLLGWANESDSVTDDKAKGWAGI HAIPRKIWLDPTGKQLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 612 Download |
| MPRPSNPIQT VPCPSLPRNA AMGTRPRGVV LAPWAVVLVL VLALRLAGAS HVIHRSLEAE 60 AAPSVPASIV SPLLRTGYHF QPPMNWINDP NAPLYYKGWY HLFYQYNPKG AVWGNIVWAH 120 SVSRDLINWV ALEPAIYPSI PSDKYGCWSG SATILEDGTP AILYTGIDRA DINYQVQVLA 180 LPKDASDPLL REWEKPEEYN PVATPAAGGI NATQFRDPTT AWRHAGHWRM LVGSVRGARG 240 MALVYRSRDF RKWTKAKHPL HSAALTGMWE CPDFFPVSGP GLQAGLDTSA PGRKYVLKSS 300 LDLTRYDYYT IGSYDGGKDR YYPDDPAGDY HHRLRYDYGN YYASKTFYDP VERRRVLLGW 360 ANESDSVTDD KAKGWAGIHA IPRKIWLDPT GKQLLQWPIH EVEKLRGKAV SVDAKLVKPG 420 DHFEVTGIAT YQADVEVSFE LELEAGTSLL EKAEAFDPAY DDDAQKLCGV KGADARGGVG 480 PFGLWVLASA DLQERTAVFF RVFRDGHGKP KVLMCTDPTK SSLSPDLYKP TFAGFVDADI 540 SSGKITLRSL IDRSVVESFG AGGKTCILSR VYPSIAVGKD AHLYVFNNGE VDVTVSGLTA 600 WEMKKPLMNG A* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 4.0e-57 | 75 | 574 | 519 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-66 | 74 | 574 | 515 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 4.0e-94 | 85 | 401 | 327 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 4.0e-143 | 79 | 398 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 79 | 563 | 493 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| Swiss-Prot | A2X5P7 | 0 | 50 | 611 | 23 | 577 | INV1_ORYSI RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 1; AltName: Full=Sucrose hydrolase 1; AltName: Full=Invertase 1; AltName: Full=Cell wall beta-fructosidase 1; AltName: Full=OsCIN1; Flags: Precursor |
| GenBank | AAD02511.1 | 0 | 51 | 611 | 32 | 591 | cell wall invertase Incw1 [Zea mays] |
| GenBank | AAP59437.1 | 0 | 74 | 611 | 1 | 529 | cell wall invertase [Saccharum hybrid cultivar Pindar] |
| GenBank | ACL53374.1 | 0 | 22 | 611 | 1 | 590 | unknown [Zea mays] |
| RefSeq | NP_001105369.1 | 0 | 22 | 611 | 1 | 590 | cell wall invertase1 precursor [Zea mays] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 75 | 604 | 9 | 536 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 75 | 603 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 75 | 603 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 75 | 603 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 75 | 603 | 5 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
