| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G163129_T01 |
| Family | GH31 |
| Protein Properties | Length: 918 Molecular Weight: 103493 Isoelectric Point: 6.0901 |
| Chromosome | Chromosome/Scaffold: 1 Start: 27094498 End: 27098727 |
| Description | Glycosyl hydrolases family 31 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 332 | 776 | 0 |
| FFFVGSEPKDVIKQYISVTGTPSMPQQFATAYHQCRWNYRDEADVDGVDAGFDEHDIPYDVLWLDIEHTDGKRYFTWDHSAFPNPEEMQRKIADKGRKMV TIVDPHIKRDSSFHLHKEATDKGYYVKDANGNDFDGWCWPGSSSYPDMLNPEIREWWADKFSYENYKGSTPTLYIWNDMNEPSVFNGPEVTMPRDAMHYG DVEHRELHNAYGYYFHMATADGLLKRGEGKDRPFVLSRAFFAGSQRYGAVWTGDNSADWDHLKSSIPMVLTLGLTGLPFSGADIGGFFGNPEPDLLVRWY QVGAFYPFFRGHAHHDTKRREPWLFGERRTAIIREAIHMRYSLLPYFYTLFREASVNGIPVMRPLWLEFPDDKETYNNGEAFMVGPSLLAQGIYEEGQKS VSVYLPGKESWYDLRNGSPYKGSATHKLQVLEDSIPSFQRAGTIV | |||
| Full Sequence |
|---|
| Protein Sequence Length: 918 Download |
| MDPPRRRLPA ALVVFLLLLA AASPVARAWK KDEFRNCNQT PFCKRARTRA PHSLDAPLSL 60 AASSLAISPD GSISAELSHP SRPRPLVLRL SALPPHALRL QIDEDYSTAT PPHRRFHVPD 120 VLLPDLEART LHLPEPKTAA GVSTVALSSD LDVVVRHDPF ELAVRRAGSG DPVLSFNSHG 180 LFDFEPMRES KPEDDTWEEH FRSHTDKRPR GPQSITFDLS FYGADFVYGL PEHGSTSLAL 240 RPTRGPGVEE SEPYRLFNLD VFEYLHESPF GLYGSIPFMI GHGGRASSGF FWLNAAEMQI 300 DVLAPGWDGV TDHENGRIDT LWMAEAGVID AFFFVGSEPK DVIKQYISVT GTPSMPQQFA 360 TAYHQCRWNY RDEADVDGVD AGFDEHDIPY DVLWLDIEHT DGKRYFTWDH SAFPNPEEMQ 420 RKIADKGRKM VTIVDPHIKR DSSFHLHKEA TDKGYYVKDA NGNDFDGWCW PGSSSYPDML 480 NPEIREWWAD KFSYENYKGS TPTLYIWNDM NEPSVFNGPE VTMPRDAMHY GDVEHRELHN 540 AYGYYFHMAT ADGLLKRGEG KDRPFVLSRA FFAGSQRYGA VWTGDNSADW DHLKSSIPMV 600 LTLGLTGLPF SGADIGGFFG NPEPDLLVRW YQVGAFYPFF RGHAHHDTKR REPWLFGERR 660 TAIIREAIHM RYSLLPYFYT LFREASVNGI PVMRPLWLEF PDDKETYNNG EAFMVGPSLL 720 AQGIYEEGQK SVSVYLPGKE SWYDLRNGSP YKGSATHKLQ VLEDSIPSFQ RAGTIVPRKD 780 RFRRSSTQMV NDPYTLVIAL NSSGAGEGEL YVDDGKSYEY QQGAFIHRRF VFADNKLTSF 840 NIGPDDLGKK FRSDCVIERI IILGLRSGVK KAIIEPGNQE LEIESGPISL RSGSSPVAPT 900 IRRPNVCIAD SWTIRIA* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06600 | GH31_MGAM-like | 1.0e-139 | 351 | 689 | 339 | + This family includes the following closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), lysosomal acid alpha-glucosidase (GAA), neutral alpha-glucosidase C (GANC), the alpha subunit of neutral alpha-glucosidase AB (GANAB), and alpha-glucosidase II. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. Alpha-glucosidase II is a GH31 enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. The MGAM-like family corresponds to subgroup 1 in the Ernst et al classification of GH31 enzymes. | ||
| cd06604 | GH31_glucosidase_II_MalA | 1.0e-158 | 351 | 689 | 346 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
| cd06603 | GH31_GANC_GANAB_alpha | 0 | 351 | 689 | 339 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
| pfam01055 | Glyco_hydro_31 | 0 | 332 | 776 | 450 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| COG1501 | COG1501 | 0 | 129 | 839 | 719 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACN34619.1 | 0 | 1 | 917 | 1 | 917 | unknown [Zea mays] |
| DDBJ | BAG72145.1 | 0 | 1 | 917 | 1 | 916 | alpha-glucosidase like protein [Hordeum vulgare subsp. vulgare] |
| GenBank | EEE58592.1 | 0 | 21 | 917 | 21 | 919 | hypothetical protein OsJ_09923 [Oryza sativa Japonica Group] |
| RefSeq | NP_001049385.1 | 0 | 58 | 917 | 1 | 862 | Os03g0216600 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002468270.1 | 0 | 1 | 917 | 1 | 917 | hypothetical protein SORBIDRAFT_01g042750 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3w38_A | 0 | 132 | 894 | 128 | 880 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3w37_A | 0 | 132 | 894 | 128 | 880 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3l4z_A | 0 | 271 | 837 | 212 | 796 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3l4y_A | 0 | 271 | 837 | 212 | 796 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
| PDB | 3l4x_A | 0 | 271 | 837 | 212 | 796 | A Chain A, Structure And Mechanisim Of Core 2 Beta1,6-N- Acetylglucosaminyltransferase: A Metal-Ion Independent Gt-A Glycosyltransferase |
