| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G167220_T01 |
| Family | AA7 |
| Protein Properties | Length: 526 Molecular Weight: 58434.8 Isoelectric Point: 6.9076 |
| Chromosome | Chromosome/Scaffold: 8 Start: 159194773 End: 159197338 |
| Description | cytokinin oxidase/dehydrogenase 1 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 58 | 257 | 1.3e-26 |
| NQCSFLPAAVLHPGSVSDIAATVRHVFSLGEGSPLTVAARGHGHSLMGQSQAAQGIVVRMESLRGPRLQVNDAGVSPPSVDAPGGELWINVLRETLKHGL APKSWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNVNQLEIVTGRGDVVTCSPDDNADLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSDFESF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 526 Download |
| MKPPSSLVHY FKLLVLLALA RLTMHVPDED VLLSLGALRL DGHFSFHDVS AMARDFGNQC 60 SFLPAAVLHP GSVSDIAATV RHVFSLGEGS PLTVAARGHG HSLMGQSQAA QGIVVRMESL 120 RGPRLQVNDA GVSPPSVDAP GGELWINVLR ETLKHGLAPK SWTDYLHLTV GGTLSNAGVS 180 GQAFRHGPQV SNVNQLEIVT GRGDVVTCSP DDNADLFYAA LGGLGQFGII TRARIALEPA 240 PKMVRWIRVL YSDFESFTED QEMLIMAENS FDYVEGFVII NRTGVLNNWR ASFKPQDPVE 300 ASHFQSDGRV LYCLELTKNF NSDDTDTMEQ EVTVLLSRLR FIQSTLFHTD VTYLEFLDRV 360 HTSELKLRAQ GLWEVPHPWL NLLIPRSSIR RFAKEVFGKI LKDSNNGPIL LYPVNKSKWD 420 NRTSVVIPDE EIFYLVGFLS SAPSLSGYGS IAHSMNLNKQ IVEFCEEAGI GMKQYLAPYT 480 TQQQWKAHFG ARWETFERRK HRYDPLAILA PGQRIFPKAS LPLPL* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 2.0e-7 | 169 | 240 | 72 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| pfam01565 | FAD_binding_4 | 3.0e-21 | 64 | 209 | 147 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 6.0e-26 | 63 | 513 | 458 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 4.0e-164 | 241 | 516 | 280 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 33 | 523 | 498 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE55201.1 | 0 | 1 | 525 | 1 | 525 | cytokinin oxidase 3 [Zea mays] |
| RefSeq | NP_001045353.1 | 0 | 1 | 525 | 5 | 529 | Os01g0940000 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001105163.1 | 0 | 1 | 525 | 1 | 525 | cytokinin oxidase 3 [Zea mays] |
| RefSeq | NP_001105526.1 | 0 | 1 | 525 | 1 | 519 | cytokinin oxidase 2 [Zea mays] |
| RefSeq | XP_002456917.1 | 0 | 1 | 525 | 1 | 523 | hypothetical protein SORBIDRAFT_03g045410 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 53 | 519 | 51 | 523 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| PDB | 2exr_A | 0 | 53 | 519 | 51 | 523 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1d_A | 0 | 34 | 516 | 19 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 34 | 516 | 19 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 34 | 516 | 19 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
