| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G394450_T01 |
| Family | GH32 |
| Protein Properties | Length: 671 Molecular Weight: 72024.9 Isoelectric Point: 4.8901 |
| Chromosome | Chromosome/Scaffold: 2 Start: 22880766 End: 22885337 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 129 | 459 | 0 |
| HFQPPKNWMNDPNGPLYHKGWYHLFYQWNPDSAVWGNITWGHAVSRDLLHWLHLPLAMVPDHPYDANGVWSGSATRLPDGRIVMLYTGSTAESSAQVQNL AEPADASDPLLREWVKSDANPVLVPPPGIGPTDFRDPTTAWRTPGNDTPAWRVAIGSKDRDHAGLALVYRTEDFVRYDPAPALMHAVPGTGMWECVDFYP VAAGSGAAADSGDGLETSAAPGPGVKHVLKASLDDDKHDYYAIGTYDPATDTWTPDSAEDDVGIGLRYDYGKYYASKTFYDPVLRRRVLWGWVGETDSER ADILKGWASVQSIPRTVLLDTKTGSNLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 671 Download |
| MIPAVADPTT LDGGGARRPL LPETDPRGRA AAGAGQKRPP ATPTVLPAVV SAVLLLVVVA 60 VTVLASQHVD GQGGGVPAGE DAVVVEVAAS RGVAEGVSEK STAQLLGAGA LQDFSWTNAM 120 LAWQRTAFHF QPPKNWMNDP NGPLYHKGWY HLFYQWNPDS AVWGNITWGH AVSRDLLHWL 180 HLPLAMVPDH PYDANGVWSG SATRLPDGRI VMLYTGSTAE SSAQVQNLAE PADASDPLLR 240 EWVKSDANPV LVPPPGIGPT DFRDPTTAWR TPGNDTPAWR VAIGSKDRDH AGLALVYRTE 300 DFVRYDPAPA LMHAVPGTGM WECVDFYPVA AGSGAAADSG DGLETSAAPG PGVKHVLKAS 360 LDDDKHDYYA IGTYDPATDT WTPDSAEDDV GIGLRYDYGK YYASKTFYDP VLRRRVLWGW 420 VGETDSERAD ILKGWASVQS IPRTVLLDTK TGSNLLQWPV VEVENLRMSG KSFDGVALDR 480 GSVVPLDVGK ATQLDIEAVF EVDASDAAAV TEADVTFNCS TSAGAAGRGL LGPFGLLVLA 540 DDDLSEQTAV YFYLLKGTDG SLQTFFCQDE LRASKANDLV KRVYGSLVPV LDGENLSVRI 600 LVDHSIVESF AQGGRTCITS RVYPTRAIYD SARVFLFNNA THAHVKAKSV KIWQLNSAYI 660 RPYPATTTSL * 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-53 | 125 | 624 | 522 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-70 | 125 | 657 | 547 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 6.0e-92 | 135 | 462 | 338 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-150 | 129 | 459 | 338 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 129 | 614 | 494 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACN34201.1 | 0 | 186 | 670 | 1 | 485 | unknown [Zea mays] |
| EMBL | CAH67112.1 | 0 | 19 | 663 | 7 | 647 | H0502G05.3 [Oryza sativa (indica cultivar-group)] |
| RefSeq | NP_001053422.1 | 0 | 1 | 663 | 1 | 654 | Os04g0535600 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | P49175 | 0 | 1 | 670 | 1 | 670 | INV1_MAIZE RecName: Full=Beta-fructofuranosidase 1; AltName: Full=Sucrose 1; AltName: Full=Invertase 1; Flags: Precursor |
| RefSeq | XP_002446857.1 | 0 | 1 | 667 | 1 | 678 | hypothetical protein SORBIDRAFT_06g023760 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 114 | 663 | 8 | 544 | A Chain A, X-Ray Crystal Structure Of Tdp-Vancosaminyltransferase Gtfd As A Complex With Tdp And The Natural Substrate, Desvancosaminyl Vancomycin. |
| PDB | 3ugh_A | 0 | 114 | 663 | 8 | 544 | A Chain A, X-Ray Crystal Structure Of Tdp-Vancosaminyltransferase Gtfd As A Complex With Tdp And The Natural Substrate, Desvancosaminyl Vancomycin. |
| PDB | 3ugg_B | 0 | 114 | 663 | 8 | 544 | A Chain A, X-Ray Crystal Structure Of Tdp-Vancosaminyltransferase Gtfd As A Complex With Tdp And The Natural Substrate, Desvancosaminyl Vancomycin. |
| PDB | 3ugg_A | 0 | 114 | 663 | 8 | 544 | A Chain A, X-Ray Crystal Structure Of Tdp-Vancosaminyltransferase Gtfd As A Complex With Tdp And The Natural Substrate, Desvancosaminyl Vancomycin. |
| PDB | 3ugf_B | 0 | 114 | 663 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
