| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G422938_T02 |
| Family | GH13 |
| Protein Properties | Length: 425 Molecular Weight: 48508.6 Isoelectric Point: 5.543 |
| Chromosome | Chromosome/Scaffold: 2 Start: 60765875 End: 60770124 |
| Description | alpha-amylase-like 2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 47 | 344 | 5.2e-34 |
| EKVTDLAESGFTSVWLPPPTQSLSREGYLPQNLYCLDSCYGSLHELKLLLHKMSEHNVRAMADVVINHRIGTTQGSNGMYNRYDGIPVSWDEHAVTSCSG GKGNKSTGDNFDGVPNIDHTQPFVRKDIIEWLIWLRKSVGFQDFRFDFTKGYAAKFVKEYIEESKPLFAVGEYWDSCEYSPPDYRLNYNQDVLVSFCLPD IHRQRIINWIDNTGGLCAAFDFTTKGILQEAVKGELWRLRDSEGKPPGVMGWWPSRSVTFIENHDTGSTQGHWPFPSDHIMEGYAYILTHPGIPTVFY | |||
| Full Sequence |
|---|
| Protein Sequence Length: 425 Download |
| MGQMISGDKS DEQTARDDGI IKNGREILFQ AFNWESNKHN WWSILEEKVT DLAESGFTSV 60 WLPPPTQSLS REGYLPQNLY CLDSCYGSLH ELKLLLHKMS EHNVRAMADV VINHRIGTTQ 120 GSNGMYNRYD GIPVSWDEHA VTSCSGGKGN KSTGDNFDGV PNIDHTQPFV RKDIIEWLIW 180 LRKSVGFQDF RFDFTKGYAA KFVKEYIEES KPLFAVGEYW DSCEYSPPDY RLNYNQDVLV 240 SFCLPDIHRQ RIINWIDNTG GLCAAFDFTT KGILQEAVKG ELWRLRDSEG KPPGVMGWWP 300 SRSVTFIENH DTGSTQGHWP FPSDHIMEGY AYILTHPGIP TVFYDHFYDQ GVPLHDEIAK 360 LMQIRKCQDI HSRSSIKILE VGSDLYSAII DDKLCMKIGD GSWCPSDPEW RLAVSGDRYA 420 VWHK* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 2.0e-44 | 23 | 366 | 426 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 2.0e-139 | 26 | 424 | 415 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 9.0e-156 | 27 | 376 | 354 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02784 | PLN02784 | 6.0e-165 | 23 | 424 | 409 | + alpha-amylase | ||
| PLN02361 | PLN02361 | 0 | 15 | 424 | 410 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE02023.2 | 0 | 4 | 424 | 1 | 412 | OSJNBb0118P14.5 [Oryza sativa (japonica cultivar-group)] |
| EMBL | CAX51375.1 | 0 | 1 | 424 | 1 | 415 | alpha-amylase [Hordeum vulgare subsp. vulgare] |
| RefSeq | NP_001052697.1 | 0 | 2 | 424 | 31 | 444 | Os04g0403300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001141482.1 | 0 | 1 | 424 | 1 | 415 | hypothetical protein LOC100273593 [Zea mays] |
| RefSeq | XP_002446386.1 | 0 | 1 | 424 | 1 | 415 | hypothetical protein SORBIDRAFT_06g015110 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1bg9_A | 0 | 26 | 424 | 1 | 402 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 1ava_B | 0 | 26 | 424 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1ava_A | 0 | 26 | 424 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1amy_A | 0 | 26 | 424 | 1 | 402 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 3bsg_A | 0 | 26 | 424 | 2 | 404 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| Hydropathy |
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