| Basic Information | |
|---|---|
| Species | Zea mays |
| Cazyme ID | GRMZM2G463871_T01 |
| Family | GH32 |
| Protein Properties | Length: 556 Molecular Weight: 59786.6 Isoelectric Point: 5.2797 |
| Chromosome | Chromosome/Scaffold: 4 Start: 240446489 End: 240449003 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 121 | 368 | 0 |
| HFQPQKNWMNDPNGPVYYKGWYHLFYQYNPDAAVWGNKIAWGHAASRDLVHWRHLPLAMVPDHWYDTNGVWTGSATTLPDGRLAMLYTGSTNASVQVQCL AVPADDADPLLTNWTKYERNPVLYPPRGIGPRDFRDPTTAWLDPSDGAWRIVIGSKDDHDHDHAGIAVVYRSRDLVHFDLLPGLLHRVVGTGMWECIDFY PVATTGGVDMSDTSIGTNDVLHVIKASMDDDRHDYYALGRYDAAANAW | |||
| Full Sequence |
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| Protein Sequence Length: 556 Download |
| MADTQPPPLP YSYVPLPAAD AASADVSGAG SRRQALCAAA LGLLAVAALA GVVPVPGRGA 60 VGMPETASSP RSSSRGPEAG VSEKTSGAWD GRIRGAAAAG GGGNAFPWSN AMLQWQRTGF 120 HFQPQKNWMN DPNGPVYYKG WYHLFYQYNP DAAVWGNKIA WGHAASRDLV HWRHLPLAMV 180 PDHWYDTNGV WTGSATTLPD GRLAMLYTGS TNASVQVQCL AVPADDADPL LTNWTKYERN 240 PVLYPPRGIG PRDFRDPTTA WLDPSDGAWR IVIGSKDDHD HDHAGIAVVY RSRDLVHFDL 300 LPGLLHRVVG TGMWECIDFY PVATTGGVDM SDTSIGTNDV LHVIKASMDD DRHDYYALGR 360 YDAAANAWTP LDAARDGVPR TVLLDTKTGA NLLQWPVEEV ETLRANSTDL SGITVDHGSV 420 FPLDLRRATQ LDIEAEFQLD RRAIAAALDD DVGYSCSTSG GAAARGALGP FGLLVLADRR 480 RRGEQTAVYF YVDGSLATHF CQDESRSSRA NDVVGSAVPV LEDEATLSLR VLVDHSIVES 540 FAQGGRSTAT SRVYS* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-49 | 117 | 554 | 501 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-67 | 117 | 554 | 483 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 7.0e-74 | 127 | 399 | 313 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-116 | 121 | 375 | 259 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 4.0e-144 | 121 | 545 | 487 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC16654.1 | 0 | 101 | 554 | 3 | 522 | soluble acid invertase [Saccharum robustum] |
| GenBank | AAC16655.1 | 0 | 101 | 554 | 3 | 522 | soluble acid invertase [Saccharum officinarum] |
| GenBank | AAP59436.1 | 0 | 67 | 554 | 40 | 595 | soluble acid invertase [Saccharum hybrid cultivar Pindar] |
| GenBank | ACG30542.1 | 0 | 3 | 554 | 5 | 626 | beta-fructofuranosidase 1 precursor [Zea mays] |
| GenBank | ACY78467.1 | 0 | 108 | 554 | 1 | 513 | soluble acid invertase [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 106 | 554 | 8 | 505 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ugh_A | 0 | 106 | 554 | 8 | 505 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ugg_B | 0 | 106 | 554 | 8 | 505 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ugg_A | 0 | 106 | 554 | 8 | 505 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ugf_B | 0 | 106 | 554 | 8 | 505 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Hydropathy |
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