| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01001124001 |
| Family | GH16 |
| Protein Properties | Length: 280 Molecular Weight: 32100.7 Isoelectric Point: 8.6468 |
| Chromosome | Chromosome/Scaffold: 1 Start: 22826373 End: 22828816 |
| Description | xyloglucan endotransglucosylase/hydrolase 5 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 58 | 209 | 1.3e-23 |
| HIKSYNGGSEIQLVLDKYTGTGFQSKGSYLFGHFSMQIKMVPGDSAGTVTAFYLSSQNSEHDEIDFEFLGNRSGQPYILQTNVFTGGKGDREQRIYLWFD PTKAFHSYSVLWNTFLIIFLVDDVPIRVFKNSKDLGVKFPFDQPMKIYSSLF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 280 Download |
| MLTTLPQSLP LGTKMASCLW LLSLGLLFMV SGARGAAPRK PIDVPFGRNY VPTWAFDHIK 60 SYNGGSEIQL VLDKYTGTGF QSKGSYLFGH FSMQIKMVPG DSAGTVTAFY LSSQNSEHDE 120 IDFEFLGNRS GQPYILQTNV FTGGKGDREQ RIYLWFDPTK AFHSYSVLWN TFLIIFLVDD 180 VPIRVFKNSK DLGVKFPFDQ PMKIYSSLFH VDGCEASVEA KFCATQGKRW WDQREFQDLD 240 SLQYRRLAWV RQKYTIYNYC TDRVRYPTMP PECKRDRDV* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00413 | Glyco_hydrolase_16 | 2.0e-21 | 53 | 213 | 176 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam06955 | XET_C | 1.0e-21 | 223 | 273 | 52 | + Xyloglucan endo-transglycosylase (XET) C-terminus. This family represents the C-terminus (approximately 60 residues) of plant xyloglucan endo-transglycosylase (XET). Xyloglucan is the predominant hemicellulose in the cell walls of most dicotyledons. With cellulose, it forms a network that strengthens the cell wall. XET catalyzes the splitting of xyloglucan chains and the linking of the newly generated reducing end to the non-reducing end of another xyloglucan chain, thereby loosening the cell wall. Note that all family members contain the pfam00722 domain. | ||
| pfam00722 | Glyco_hydro_16 | 5.0e-64 | 46 | 208 | 165 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 2.0e-80 | 39 | 273 | 267 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 3.0e-139 | 43 | 273 | 261 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005618 | cell wall |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006073 | cellular glucan metabolic process |
| GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC09388.1 | 0 | 15 | 279 | 1 | 293 | xyloglucan endotransglycosylase precursor [Actinidia deliciosa] |
| GenBank | ACD03215.1 | 0 | 15 | 279 | 1 | 293 | xyloglucan endotransglucosylase/hydrolase 5 [Actinidia deliciosa] |
| EMBL | CBI29290.1 | 0 | 1 | 279 | 1 | 279 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002274520.1 | 0 | 1 | 279 | 1 | 307 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002304625.1 | 0 | 16 | 279 | 3 | 294 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 36 | 279 | 7 | 278 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 1un1_A | 0 | 36 | 279 | 7 | 278 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 1umz_B | 0 | 36 | 279 | 7 | 278 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 36 | 279 | 7 | 278 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2vh9_B | 0 | 19 | 273 | 8 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
