| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01001272001 |
| Family | GH32 |
| Protein Properties | Length: 472 Molecular Weight: 52154.6 Isoelectric Point: 4.8099 |
| Chromosome | Chromosome/Scaffold: 2 Start: 4882099 End: 4884289 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 3 | 262 | 0 |
| ADQWYDTNGVWTGSATLLSDGQVIMLYTGATNESVQVQNLAYPADLSDPLLVDWVKYPGNPVLVPPPGIDDRDFRDPTTAWYWPDGKWRIAIGSKVNKTG ISLVYNTEDFKKYELIEGVLHAVPGTGMWECVDLYPVSLKEDNGLDTSFNGPGVKHVLKASLDDDKNDYYAIGTYSLESGNWTPDNSNLDVGIGLRYDYG KFYASKTFYDQNKQRRILWGWIGETDGESADIKKGWASVQSIPRTVVFDKKTGTNILQWP | |||
| Full Sequence |
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| Protein Sequence Length: 472 Download |
| MVADQWYDTN GVWTGSATLL SDGQVIMLYT GATNESVQVQ NLAYPADLSD PLLVDWVKYP 60 GNPVLVPPPG IDDRDFRDPT TAWYWPDGKW RIAIGSKVNK TGISLVYNTE DFKKYELIEG 120 VLHAVPGTGM WECVDLYPVS LKEDNGLDTS FNGPGVKHVL KASLDDDKND YYAIGTYSLE 180 SGNWTPDNSN LDVGIGLRYD YGKFYASKTF YDQNKQRRIL WGWIGETDGE SADIKKGWAS 240 VQSIPRTVVF DKKTGTNILQ WPVAEIKSLR KSSKKFDKLE VGPGSVVTLE VEKATQMDIT 300 AEFEIDKEAL KRIGESDVEY SCGTSGGSAQ RGELGPFGLL LLADEGRCEQ TPVYFYVAKG 360 TDGQLKTFFC TDESRSSLAN DVDKRIFGST VPVIKGEKLS MRILVDHSII ESFAQGGRTC 420 ITSRVYPTKA IYGAAQLYVF NNATSASITA SIQTWAMKSA YIRPYSSHQE S* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-31 | 4 | 427 | 439 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 3.0e-41 | 4 | 428 | 439 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-55 | 4 | 265 | 268 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 6.0e-106 | 1 | 262 | 270 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-143 | 1 | 417 | 427 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB47172.1 | 0 | 1 | 471 | 194 | 664 | vacuolar invertase 2, GIN2 [Vitis vinifera=grape berries, Sultana, berries, Peptide, 664 aa] |
| EMBL | CBI31885.1 | 0 | 1 | 471 | 1 | 471 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002272774.1 | 0 | 1 | 471 | 134 | 604 | PREDICTED: similar to vacuolar invertase 2, GIN2 isoform 1 [Vitis vinifera] |
| RefSeq | XP_002272809.1 | 0 | 1 | 471 | 47 | 517 | PREDICTED: similar to vacuolar invertase 2, GIN2 isoform 2 [Vitis vinifera] |
| RefSeq | XP_002303519.1 | 0 | 1 | 470 | 193 | 662 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 3ugh_A | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 3ugg_B | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 3ugg_A | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
| PDB | 3ugf_B | 0 | 1 | 465 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EE065992 | 255 | 217 | 471 | 0 |
| CB969297 | 254 | 219 | 472 | 0 |
| CB970781 | 246 | 219 | 464 | 0 |
| EL449640 | 261 | 1 | 261 | 0 |
| DY919082 | 262 | 1 | 262 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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