| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01008673001 |
| Family | GH13 |
| Protein Properties | Length: 841 Molecular Weight: 95163 Isoelectric Point: 5.0564 |
| Chromosome | Chromosome/Scaffold: 18 Start: 1101732 End: 1110711 |
| Description | starch branching enzyme 2.2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 293 | 618 | 4.4e-30 |
| LPRIRANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLRVLMDVVHSHASNNVTDGLNGFDVGQSSQDSYFHTGDRGYHE LWDSKLFNYANWEVLRFLISNLRWWLEEFKFDGFRFDGVTSMLYHHHGVNMTFTGNYNEYFSEATDVDAVVYLMLANCLIHKIFPDATVSAEDVSGMPGL GRPVAEGGTGFDYRLAMAIPDKWIDYLKNKKDEEWSMKEISSSLTNRRYAEKCISYAESHDQALVGDKTIAFLLMDKEMYSGMSCLTDASPTIDRGISLH KMIHFITMALGGEGFLNFMGNEFGHP | |||
| Full Sequence |
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| Protein Sequence Length: 841 Download |
| MYGSVGLFST PLLGSSSTPS SRNHYKTGSG NKYCLAKQQP IRVSNGPRLQ KLLGHCTFHC 60 PSRNSTYGKV NGSAISALIT DDNSAMATMG EDTEHIGILD TDPGLEPFKD HFRYRMRRYV 120 EQKELIEKYE GSLEEFAQGY LKFGFNREEG GIVYREWAPA AQEAQVIGDF NGWDGSNHRM 180 ERNQFGVWSI KIPDSGGNPA IPHNSRVKFR FKHGDGVWVD RIPAWIRYAT VDPTAFAAPY 240 DGVYWDPPPS ERYQFKYPCP SKPNAPRIYE AHVGMSSSEP RVNSYREFAD DILPRIRANN 300 YNTVQLMAVM EHSYYASFGY HVTNFFAVSS RSGTPEDLKY LIDKAHSLGL RVLMDVVHSH 360 ASNNVTDGLN GFDVGQSSQD SYFHTGDRGY HELWDSKLFN YANWEVLRFL ISNLRWWLEE 420 FKFDGFRFDG VTSMLYHHHG VNMTFTGNYN EYFSEATDVD AVVYLMLANC LIHKIFPDAT 480 VSAEDVSGMP GLGRPVAEGG TGFDYRLAMA IPDKWIDYLK NKKDEEWSMK EISSSLTNRR 540 YAEKCISYAE SHDQALVGDK TIAFLLMDKE MYSGMSCLTD ASPTIDRGIS LHKMIHFITM 600 ALGGEGFLNF MGNEFGHPEW IDFPREGNDW SYEKCRRQWE LVDTDHLRYK YMNAFDTAMN 660 LLDEKFSFLA STKQIVSSTD EEHKVIVFER GDLVFVFNFH PENTYDGYKV GCDLPGKYRV 720 ALDSDACVFG GQGRVGHDAD HFTSPEGIPG VPETNFNNRP NSFKVLSPAR TCVVYYRVEE 780 SLEESDDDHN STGANATLVA DVVAEQESLE EPASVKDHEF KPRLIEGSEV EDVVEEATDD 840 * 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 5.0e-10 | 108 | 291 | 202 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 9.0e-140 | 199 | 775 | 581 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 37 | 805 | 769 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 249 | 656 | 408 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 199 | 776 | 580 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABN05321.1 | 0 | 32 | 825 | 29 | 819 | starch branching enzyme I [Populus trichocarpa] |
| GenBank | ACM07441.1 | 0 | 1 | 784 | 1 | 784 | starch-branching enzyme I [Nelumbo nucifera] |
| EMBL | CAA54308.1 | 0 | 1 | 817 | 1 | 831 | 1,4-alpha-glucan branching enzyme [Manihot esculenta] |
| EMBL | CBI18866.1 | 0 | 1 | 840 | 1 | 840 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002284841.1 | 0 | 28 | 840 | 5 | 817 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3aml_A | 0 | 86 | 784 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3amk_A | 0 | 86 | 784 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 86 | 784 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 86 | 784 | 1 | 699 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 1m7x_D | 2.8026e-45 | 151 | 731 | 26 | 577 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO619167 | 639 | 182 | 819 | 0 |
| HO794536 | 690 | 98 | 775 | 0 |
| HO777638 | 638 | 150 | 775 | 0 |
| CX109187 | 390 | 395 | 784 | 0 |
| HO777638 | 46 | 102 | 147 | 1.9 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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