| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01009786001 |
| Family | AA7 |
| Protein Properties | Length: 580 Molecular Weight: 64809.5 Isoelectric Point: 7.7173 |
| Chromosome | Chromosome/Scaffold: 18 Start: 11256600 End: 11261386 |
| Description | cytokinin oxidase 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 103 | 302 | 2.8e-30 |
| VDFGMMNRAEPLAVLHPSSAEDVARLVGAAYGSAHGLSVSARGHGHSINGQAQTSSGVVIEMSASKGVRQWGLPRVSEQSRYVDAWGGELWIDVLKTTLE HGLAPKSWTDYLYLSVGGTLSNAGISGQAFNHGPQISNVYELDVVTGKGELLTCSEEQNSELFHAVLGGLGQFGIITRARIGLEPAPQRVRWIRVLYSNF | |||
| Full Sequence |
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| Protein Sequence Length: 580 Download |
| MLRGKSLKRN PAYVCSTTPH DAATRFCSLP TTTRTQIPSA RKKYTNQRSR GERKHMAPKL 60 LLTFVIYRLI STVGLTLDPT DLLRLEVDGH LSVDPSDVKT ASVDFGMMNR AEPLAVLHPS 120 SAEDVARLVG AAYGSAHGLS VSARGHGHSI NGQAQTSSGV VIEMSASKGV RQWGLPRVSE 180 QSRYVDAWGG ELWIDVLKTT LEHGLAPKSW TDYLYLSVGG TLSNAGISGQ AFNHGPQISN 240 VYELDVVTGK GELLTCSEEQ NSELFHAVLG GLGQFGIITR ARIGLEPAPQ RVRWIRVLYS 300 NFSTFTKDQE YLISLHGQPP NQKFDYVEGF VIVDEGLINN WRSSFFSPRN PVKISSFGTN 360 GGVLYCLEVT KNYHESTADT IDQDVEALLK RLDFIPSSVF TTDLPYVDFL DRVHKAELKL 420 RSKGLWDVPH PWLNLFVPRS RIADFDEGVF KGILGNKTSG PILIYPMNKN KWDDRTSVVT 480 PEEDVFYLVA LLRSALDSGD EAQSLEYLSN QNRQILRFCD DAGIKVKQYL PHYTTQEDWV 540 DHFGDDKWTL FSKRKMDFDP RRILATGQRI FKPMAASSW* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 2.0e-11 | 111 | 312 | 207 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| pfam01565 | FAD_binding_4 | 8.0e-27 | 113 | 257 | 146 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 4.0e-29 | 88 | 568 | 487 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 3.0e-167 | 289 | 571 | 285 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 59 | 578 | 524 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN81010.1 | 0 | 56 | 579 | 1 | 515 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI19763.1 | 0 | 1 | 579 | 1 | 579 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002280797.1 | 0 | 56 | 579 | 1 | 524 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002300742.1 | 0 | 56 | 578 | 1 | 528 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002307681.1 | 0 | 56 | 578 | 1 | 528 | cytokinin oxidase [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3s1d_A | 0 | 79 | 572 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 79 | 572 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 79 | 572 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3c0p_A | 0 | 79 | 572 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3bw7_A | 0 | 79 | 572 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CF209921 | 256 | 325 | 580 | 0 |
| DY269838 | 329 | 113 | 434 | 0 |
| EC922019 | 248 | 333 | 580 | 0 |
| DT457117 | 297 | 113 | 403 | 0 |
| DY284491 | 295 | 56 | 342 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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