| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01014092001 |
| Family | GT20 |
| Protein Properties | Length: 881 Molecular Weight: 99104.5 Isoelectric Point: 6.5432 |
| Chromosome | Chromosome/Scaffold: 19 Start: 292203 End: 312996 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 85 | 553 | 0 |
| ARQRLLVVANRLPVSAIRRGEESWSLEISAGGLVSALLGVKEFEARWIGWAGVNVPDEAGQRALTKALAEKMCIPVFLDEDIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNKHYEEGDVVWCHDYHLMFLPKCLKKYNSEMKVGWFLHTPFPSSEIHRTLPSRSELLHSVLAADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSHRFIRALDAPQVQDRINELKRTFTGRKVMLGVDRLDMIKGIPQKILAFEKFLEE NSEWQQKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFYALCALYAVTDVALVTSLRDGMNLVSYEFVACQESKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVASSIAQALNMPPEEREKRHEHNFEHVKNHTAQEWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 881 Download |
| MPGNKYNGIS SVPTSRVERL LRDRELRKSS RASHPNEAND TNRGTEVLEH EGDNLGSSHV 60 EQHSETAAIR GVLSEECEKQ ETRPARQRLL VVANRLPVSA IRRGEESWSL EISAGGLVSA 120 LLGVKEFEAR WIGWAGVNVP DEAGQRALTK ALAEKMCIPV FLDEDIVHQY YNGYCNNILW 180 PLFHYLGLPQ EDRLATTRSF QSQFAAYKKA NQMFADVVNK HYEEGDVVWC HDYHLMFLPK 240 CLKKYNSEMK VGWFLHTPFP SSEIHRTLPS RSELLHSVLA ADLVGFHTYD YARHFVSACT 300 RILGLEGTPE GVEDQGRLTR VAAFPIGIDS HRFIRALDAP QVQDRINELK RTFTGRKVML 360 GVDRLDMIKG IPQKILAFEK FLEENSEWQQ KVVLLQIAVP TRTDVPEYQK LTSQVHEIVG 420 RINGRFGTLT AVPIHHLDRS LDFYALCALY AVTDVALVTS LRDGMNLVSY EFVACQESKK 480 GVLILSEFAG AAQSLGAGAI LVNPWNITEV ASSIAQALNM PPEEREKRHE HNFEHVKNHT 540 AQEWAETFVS ELNDTVVEAD LRKRKVPPRL TPENAIKCYL QSNNRLLILG FNVTLTEPVD 600 TPGSRGGDQI KEMDLKLHPE LKGPLTALGN DPKTTIVVLS GSDRTVLDDN FGEFDMWLAA 660 ENGMFLRHTK GEWMTTMPEH LNMEWVDSVK HVFEYFTERT PRSQLQKRET SLVWNYKYAD 720 AEFGKLQARD MLQHLWTGPI SNASVDVVQG SRSVEVRAVG VTKGAAIDRI LGEIVHNKSM 780 TSPIDYVLCA GHFLGKDEDV YTFFEPELPS DNISMAKSRA IDAFKRPSPE KTSWNVLDLK 840 GENYFSCAVG RTRTKARFTF GSSDEVVSFL KELAGAKSSF * 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 880 | 934 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 81 | 879 | 799 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PRK14501 | PRK14501 | 0 | 88 | 874 | 793 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 88 | 554 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 87 | 554 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN79485.1 | 0 | 1 | 880 | 1 | 913 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI20106.1 | 0 | 1 | 880 | 1 | 880 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002285632.1 | 0 | 1 | 880 | 1 | 927 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 878 | 1 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 878 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 88 | 556 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 88 | 556 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 88 | 556 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 88 | 556 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 88 | 556 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 388 | 449 | 834 | 0 |
| CX546763 | 305 | 74 | 377 | 0 |
| CV711307 | 294 | 85 | 378 | 0 |
| DW069910 | 288 | 174 | 461 | 0 |
| CX545499 | 306 | 391 | 696 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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