| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01015194001 |
| Family | AA7 |
| Protein Properties | Length: 495 Molecular Weight: 55169.2 Isoelectric Point: 7.4649 |
| Chromosome | Chromosome/Scaffold: 11 Start: 1702553 End: 1704880 |
| Description | cytokinin oxidase 3 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 66 | 232 | 1.6e-22 |
| FTIAARGRGHSLGGQAMAPNGVVVDMTSLKNSGAGIGIKVTKNPVSGSYADVGGHQLWIDVLQATLEHGLAPVSWTDYLYLTVGGTLSNAGGSGQTFRHG PQISNVHEMDIITGKGELVTCSKETNSDLFYAVLGGLGQFGIITRARIPLEPAPKRVKWVRMLYDDF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 495 Download |
| MAKTYSIPTY FILISFLINL ISSLTLTWAD VLPHQLHTLT IATRLRVDLD ATAKAARDFG 60 KLPSPFTIAA RGRGHSLGGQ AMAPNGVVVD MTSLKNSGAG IGIKVTKNPV SGSYADVGGH 120 QLWIDVLQAT LEHGLAPVSW TDYLYLTVGG TLSNAGGSGQ TFRHGPQISN VHEMDIITGK 180 GELVTCSKET NSDLFYAVLG GLGQFGIITR ARIPLEPAPK RVKWVRMLYD DFSTFSEDQE 240 HLISINGLDY LEGSLITKQS PPNNWRSSFF SKSQYPIISS LLTKNGIIYS IEVVKYYDDL 300 TSHTVDEELQ ELFKGLRFLP GLVFTKDVTL VDFLDRVHNG ELQLQAKGLW DVPHPWLNLF 360 VPKSRISDFN SGVFRDIILK TNQTVGPLLV YPMIRNKWDN GMSAAIPDED IFYSIGLLHS 420 SGADDWEPLE NQNKEILKFC DKAGIKIKQY LPRYTTKADW MNHFGPKWKI FEDRKAQFDP 480 KNILSPGQRI FNSI* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 2.0e-7 | 147 | 218 | 72 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| pfam01565 | FAD_binding_4 | 2.0e-21 | 48 | 187 | 141 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 3.0e-29 | 48 | 488 | 453 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 1.0e-142 | 219 | 491 | 281 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 38 | 494 | 490 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN80714.1 | 0 | 1 | 494 | 1 | 522 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI27904.1 | 0 | 1 | 494 | 1 | 494 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002264445.1 | 0 | 1 | 494 | 1 | 522 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002264539.1 | 0 | 1 | 494 | 1 | 522 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002279519.1 | 0 | 1 | 494 | 1 | 521 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3s1d_A | 0 | 33 | 492 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 33 | 492 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 33 | 492 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3c0p_A | 0 | 33 | 492 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3bw7_A | 0 | 33 | 492 | 17 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
