| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01018625001 |
| Family | GH32 |
| Protein Properties | Length: 543 Molecular Weight: 60556.1 Isoelectric Point: 4.2315 |
| Chromosome | Chromosome/Scaffold: 16 Start: 12049723 End: 12054218 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 10 | 329 | 0 |
| HFQPEKNWMNGRNALYPDGPLFHMGWYHLFYQYNPDSAVWGNITWGHAVSRDMIHWLYLPLAMVPDRWFDLNGVWTGSATILPNGQIIMLYTGDTNDSVQ VQNLAYPANLSDPLLLHWIKYENNPVMVPPAGIGSDDFRDPTTMWVGADGNWRVAVGSLVNTTGIVLVFQTTNFTDFELLDGELHGVPGTGMWECVDFYP VSINGVYGLDTSAHGPGIKHVLKASMDDNRHDYYALGEYDPMTDTWTPDDPELDVGIGLRLDYGKYYASKTFYDQVKKRRILYGWISEGDIESDDLKKGW ASLQSIPRTVLHDNKTGTYL | |||
| Full Sequence |
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| Protein Sequence Length: 543 Download |
| MFAWQRTAFH FQPEKNWMNG RNALYPDGPL FHMGWYHLFY QYNPDSAVWG NITWGHAVSR 60 DMIHWLYLPL AMVPDRWFDL NGVWTGSATI LPNGQIIMLY TGDTNDSVQV QNLAYPANLS 120 DPLLLHWIKY ENNPVMVPPA GIGSDDFRDP TTMWVGADGN WRVAVGSLVN TTGIVLVFQT 180 TNFTDFELLD GELHGVPGTG MWECVDFYPV SINGVYGLDT SAHGPGIKHV LKASMDDNRH 240 DYYALGEYDP MTDTWTPDDP ELDVGIGLRL DYGKYYASKT FYDQVKKRRI LYGWISEGDI 300 ESDDLKKGWA SLQSIPRTVL HDNKTGTYLL LWPIEEVESL RTNSTEFEDV LLEPGSVVPL 360 DIGSASQLDI VAEFEVDNET LEAMVEADVI YNCSTSAGAA GRGALGPFGI LVLADDTLSE 420 LTPIYFYIAK DTDGSYKTFF CTDLSRSSLA VDDVDQRIYG SIVPVLDDEK PTMRVLVDHS 480 IVEGFSQGGR SCITTRVYPT EAIYGAARLF LFNNATGVNV TASIKIWEMA SADIHPYPLD 540 QP* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-44 | 6 | 499 | 513 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-63 | 6 | 527 | 539 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-83 | 16 | 336 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-134 | 10 | 333 | 332 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-172 | 10 | 489 | 489 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB47171.1 | 0 | 1 | 542 | 106 | 642 | vacuolar invertase 1, GIN1 [Vitis vinifera=grape berries, Sultana, berries, Peptide, 642 aa] |
| GenBank | ABI17894.1 | 0 | 1 | 538 | 52 | 584 | vacuolar invertase [Coffea canephora] |
| EMBL | CAA53099.1 | 0 | 1 | 541 | 126 | 660 | beta-fructofuranosidase [Daucus carota] |
| EMBL | CBI24585.1 | 0 | 1 | 542 | 1 | 542 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002265534.1 | 0 | 1 | 542 | 113 | 649 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 1 | 537 | 14 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3ugh_A | 0 | 1 | 537 | 14 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3ugg_B | 0 | 1 | 537 | 14 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3ugg_A | 0 | 1 | 537 | 14 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| PDB | 3ugf_B | 0 | 1 | 537 | 14 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
| fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EC920118 | 308 | 221 | 528 | 0 |
| EC920124 | 257 | 261 | 517 | 0 |
| CB973653 | 260 | 279 | 538 | 0 |
| EC920557 | 279 | 261 | 539 | 0 |
| ES821884 | 365 | 5 | 362 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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