| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01020215001 |
| Family | GT20 |
| Protein Properties | Length: 644 Molecular Weight: 72672.9 Isoelectric Point: 6.9328 |
| Chromosome | Chromosome/Scaffold: 1 Start: 8958843 End: 8962074 |
| Description | trehalose phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 31 | 141 | 1.8e-23 |
| MTRKIIVANMLPLHAQRDKVTAKWCFSLDEDALLLHLKDGFSPETEVIYVGSLKVEIDASEQEEVAQKLLEDFNCVPTFLPHDLHKKFYHGFCKQQLWPL FHYMLPMKRFH | |||
| GT20 | 138 | 377 | 0 |
| KRFHRVKLGFFLHSPFPSSEIYRTLPVRDEILRGLLNCDLIGFQTFDYARHFLSCCSRMLGLDYESKRGHIGLDYSGRTVYIKILPVGVHMGRLESVLNL HSTSAKIKEIQKQFEGKKLILGVDDMDIFKGISLKFLAVEQLLQQHPELQGKLVLVQIVNPARSTGKDVQEAKRETYLTAERINETYGSPNYEPVILIDR PVARYEKSAYYAVAECCIVNAVRDGMNLVPYKYIVCRQGT | |||
| Full Sequence |
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| Protein Sequence Length: 644 Download |
| MASRSCANFL DLASGSLLDI PHTPRPLPRV MTRKIIVANM LPLHAQRDKV TAKWCFSLDE 60 DALLLHLKDG FSPETEVIYV GSLKVEIDAS EQEEVAQKLL EDFNCVPTFL PHDLHKKFYH 120 GFCKQQLWPL FHYMLPMKRF HRVKLGFFLH SPFPSSEIYR TLPVRDEILR GLLNCDLIGF 180 QTFDYARHFL SCCSRMLGLD YESKRGHIGL DYSGRTVYIK ILPVGVHMGR LESVLNLHST 240 SAKIKEIQKQ FEGKKLILGV DDMDIFKGIS LKFLAVEQLL QQHPELQGKL VLVQIVNPAR 300 STGKDVQEAK RETYLTAERI NETYGSPNYE PVILIDRPVA RYEKSAYYAV AECCIVNAVR 360 DGMNLVPYKY IVCRQGTPHM DKETGMNLQL GKRCWGIVIS VLSTLCSDPK NTVFIVSGRG 420 RSSLSEWLAP CERLGIAAEH GYFMRWNEST KWESCNLAAD LDWKKVVEPV MRLYTETTDG 480 SNIEIKESAL VWHHQDADPD FGSCQAKELM DHLENVLANE PAVVKRGQHI VEVKPQGVSK 540 GLVAEKVLST MVNDGKPPDF VMCIGDDRSD EDMFEGIFRT ISSPSLSMSP EIFACTVGQK 600 PSKAKYYLDD TTDVVRLLQG LATASNPKPR YIAQIQVSFE SSI* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam02358 | Trehalose_PPase | 1.0e-90 | 398 | 611 | 215 | + Trehalose-phosphatase. This family consist of trehalose-phosphatases EC:3.1.3.12 these enzyme catalyze the de-phosphorylation of trehalose-6-phosphate to trehalose and orthophosphate. The aligned region is present in trehalose-phosphatases and comprises the entire length of the protein it is also found in the C-terminus of trehalose-6-phosphate synthase EC:2.4.1.15 adjacent to the trehalose-6-phosphate synthase domain - pfam00982. It would appear that the two equivalent genes in the E. coli otsBA operon otsA the trehalose-6-phosphate synthase and otsB trehalose-phosphatase (this family) have undergone gene fusion in most eukaryotes. Trehalose is a common disaccharide of bacteria, fungi and invertebrates that appears to play a major role in desiccation tolerance. | ||
| PLN02205 | PLN02205 | 6.0e-109 | 399 | 626 | 228 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| cd03788 | GT1_TPS | 7.0e-132 | 35 | 378 | 391 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| pfam00982 | Glyco_transf_20 | 1.0e-160 | 33 | 376 | 397 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PLN02205 | PLN02205 | 3.0e-178 | 1 | 393 | 478 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN78769.1 | 0 | 1 | 388 | 1 | 464 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI32125.1 | 0 | 1 | 643 | 1 | 643 | unnamed protein product [Vitis vinifera] |
| GenBank | EEC84489.1 | 0 | 1 | 639 | 1 | 760 | hypothetical protein OsI_31155 [Oryza sativa Indica Group] |
| RefSeq | XP_002264873.1 | 0 | 1 | 388 | 1 | 464 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002264873.1 | 0 | 398 | 630 | 612 | 844 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1uqu_B | 1e-29 | 117 | 467 | 75 | 455 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 1uqu_A | 1e-29 | 117 | 467 | 75 | 455 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 1uqt_B | 1e-29 | 117 | 467 | 75 | 455 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 1uqt_A | 1e-29 | 117 | 467 | 75 | 455 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| PDB | 2wtx_D | 1e-29 | 117 | 467 | 75 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO779327 | 364 | 72 | 382 | 0 |
| HO779327 | 246 | 399 | 644 | 0 |
| GR925301 | 250 | 138 | 387 | 0 |
| HO777775 | 389 | 54 | 386 | 0 |
| HO777775 | 227 | 399 | 625 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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