| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01021111001 |
| Family | GT35 |
| Protein Properties | Length: 815 Molecular Weight: 92892.9 Isoelectric Point: 5.8642 |
| Chromosome | Chromosome/Scaffold: 10 Start: 1507666 End: 1557047 |
| Description | alpha-glucan phosphorylase 2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 96 | 808 | 0 |
| ALSQLGFEYEVLAEQEGDAALGNGGLARLSACQMDSLATLDYPAWGYGLRYQYGLFRQVILDGFQHEQPDYWLNFGNPWEIERVHVSYPVKFYGTVEEET LNGKSCKVWLPGETVEAVAYDNPIPGYGTRNTINLRLWAAKPDGQYDMESYNTGDYINAVVNRQRAETISCVLYPDDRSYQGKELRLKQHYFFVSASLQD IIRRFKDGHNNFDDFPEKVALQLNDTHPSLAVVEVMRVLVDEEHLGWDQAWNIVCRIFSFTTHTVLPEALEKIPVDLLGSLLPRHLQIIYDINFNFMEEL KKRIGLDFNRLSQMSIVEEGAVKSIRMANLSIVCSHTVNGVSRMHSELLKTRVFKDFYELWPHKFQYKTNGVTQRRWIVVSNPSLCALISKWLGTEAWIR DIDLLIGLQEFAADADLHQEWKMVRKVNKMRLAEYIEAMSGVKVSLDAMFDVQIKRIHEYKRQLLNILSIIHRYDCIKNMEKTQRRKVVPRVCIVGGKAA PGYEVAKKIIKLCHAVAEKINNDADVGDLLKLIFVPDYNVSVAELVIPGADLSQHISTAGHEASGTGCMKFLMNGCLLLATADGSTVEIIEEIGEENMFL FGAKVHEVPALREKSSDHKAPLQFSHVVRMVRDGHFGFKDYFKSLCDGVEGDSDFYLLGSDFASYLEAQAAADKAFVDQEKWTQMSILSTAGSGRFSSDR TIEDYAETTWGIE | |||
| Full Sequence |
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| Protein Sequence Length: 815 Download |
| MDRFLSNDPV SLQKDILDHV EYTVARSRFS FDDFEAYQAL AHSVRDRLIE RWHDTQQYFK 60 RKDPKRLYFL SLEFLMGRSL SNSVINLGIR DQCADALSQL GFEYEVLAEQ EGDAALGNGG 120 LARLSACQMD SLATLDYPAW GYGLRYQYGL FRQVILDGFQ HEQPDYWLNF GNPWEIERVH 180 VSYPVKFYGT VEEETLNGKS CKVWLPGETV EAVAYDNPIP GYGTRNTINL RLWAAKPDGQ 240 YDMESYNTGD YINAVVNRQR AETISCVLYP DDRSYQGKEL RLKQHYFFVS ASLQDIIRRF 300 KDGHNNFDDF PEKVALQLND THPSLAVVEV MRVLVDEEHL GWDQAWNIVC RIFSFTTHTV 360 LPEALEKIPV DLLGSLLPRH LQIIYDINFN FMEELKKRIG LDFNRLSQMS IVEEGAVKSI 420 RMANLSIVCS HTVNGVSRMH SELLKTRVFK DFYELWPHKF QYKTNGVTQR RWIVVSNPSL 480 CALISKWLGT EAWIRDIDLL IGLQEFAADA DLHQEWKMVR KVNKMRLAEY IEAMSGVKVS 540 LDAMFDVQIK RIHEYKRQLL NILSIIHRYD CIKNMEKTQR RKVVPRVCIV GGKAAPGYEV 600 AKKIIKLCHA VAEKINNDAD VGDLLKLIFV PDYNVSVAEL VIPGADLSQH ISTAGHEASG 660 TGCMKFLMNG CLLLATADGS TVEIIEEIGE ENMFLFGAKV HEVPALREKS SDHKAPLQFS 720 HVVRMVRDGH FGFKDYFKSL CDGVEGDSDF YLLGSDFASY LEAQAAADKA FVDQEKWTQM 780 SILSTAGSGR FSSDRTIEDY AETTWGIEPC KCPS* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 13 | 807 | 801 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 16 | 807 | 798 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| pfam00343 | Phosphorylase | 0 | 96 | 809 | 720 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| COG0058 | GlgP | 0 | 11 | 809 | 812 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| PRK14986 | PRK14986 | 0 | 43 | 811 | 775 | + glycogen phosphorylase; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI30609.1 | 0 | 1 | 814 | 1 | 814 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_001757919.1 | 0 | 1 | 811 | 1 | 811 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_002273615.1 | 0 | 1 | 814 | 5 | 818 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002305114.1 | 0 | 1 | 813 | 5 | 817 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002509431.1 | 0 | 1 | 813 | 188 | 948 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1ygp_B | 0 | 7 | 810 | 39 | 878 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 1ygp_A | 0 | 7 | 810 | 39 | 878 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 3l7d_A | 0 | 10 | 811 | 26 | 832 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 3l7c_A | 0 | 10 | 811 | 26 | 832 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| PDB | 3l7b_A | 0 | 10 | 811 | 26 | 832 | A Chain A, Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO779924 | 523 | 1 | 521 | 0 |
| HO586252 | 541 | 272 | 810 | 0 |
| HO376977 | 446 | 369 | 813 | 0 |
| HO417459 | 294 | 423 | 716 | 0 |
| HO417459 | 212 | 224 | 429 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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