| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01021313001 |
| Family | GT20 |
| Protein Properties | Length: 488 Molecular Weight: 54786.9 Isoelectric Point: 6.7526 |
| Chromosome | Chromosome/Scaffold: 10 Start: 3883061 End: 3911610 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 18 | 163 | 0 |
| NQKLTSQVHEIVGRINGRFGTLTTVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAILVNPWNI TEVAASIGYALNMPADEREKRHWHNFMHVTTHTSQEWALTFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 488 Download |
| MMVITKFFAL KVNYYLRNQK LTSQVHEIVG RINGRFGTLT TVPIHHLDRS LDFHALCALY 60 AVTDVALVTS LRDGMNLVSY EFVACQASKK GVLILSEFAG AAQSLGAGAI LVNPWNITEV 120 AASIGYALNM PADEREKRHW HNFMHVTTHT SQEWALTFVS ELNDTIFEAQ LRTRQVPPLL 180 PTKDAIERYL QSNNRLLILG FNSTLTEPVD IPGRRGGQIK EMELKLHPDL KEPLKKLCDD 240 PKTTVIILSG SDRGVLDDNF SEYNMWLAAE NGMFLRLTTG EWMTTMPDNL NMDWVDSVKH 300 VFEYFTERTP RSHFELRETS LVWNYKYADI EFGRLQARDM LQHLWTGPIS NASVDVVQGG 360 RSVEVRAVGV TKGAAIDRIL GEIVHNRGMK APIDYVLCIG HFLGKDEDIY TFFEPELPSE 420 PAAAARTKVV EPVKTSATKV STILDLKGDN YFSCAVGRKR SKARYLLGSL DDVVTLIKDL 480 AEFSRPC* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR02400 | trehalose_OtsA | 3.0e-74 | 19 | 164 | 146 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 2.0e-75 | 19 | 164 | 146 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 1.0e-105 | 19 | 482 | 465 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| PLN03064 | PLN03064 | 0 | 19 | 486 | 517 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 19 | 481 | 463 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 19 | 482 | 413 | 918 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| EMBL | CBI30769.1 | 0 | 1 | 487 | 1 | 487 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002305707.1 | 0 | 19 | 486 | 388 | 898 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002331300.1 | 0 | 19 | 484 | 409 | 921 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522296.1 | 0 | 19 | 484 | 404 | 912 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 9e-26 | 19 | 166 | 307 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 9e-26 | 19 | 166 | 307 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 9e-26 | 19 | 166 | 307 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 9e-26 | 19 | 166 | 307 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1uqu_B | 1e-25 | 19 | 174 | 308 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 355 | 59 | 413 | 0 |
| DV705833 | 283 | 131 | 413 | 0 |
| CX545499 | 287 | 19 | 305 | 0 |
| GO265628 | 303 | 53 | 355 | 0 |
| GE582665 | 283 | 34 | 315 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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