| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01031857001 |
| Family | GT20 |
| Protein Properties | Length: 578 Molecular Weight: 66439.3 Isoelectric Point: 7.1704 |
| Chromosome | Chromosome/Scaffold: 3 Start: 4943035 End: 4950015 |
| Description | trehalose-phosphatase/synthase 7 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 58 | 300 | 0 |
| QDRLIIVANQLPVKANRRADDRGWVFSWNEDSLLLQLKEEEVSQILLETFKCVPTFLPHDVLEKFYHGFCKKLLWPLFHYMLPFSADHGGRFDRSMWEAY VWANKLFSQKVIEAINPDDDYVWIHDYHLMVLPTFLRRHFNQLRMGFFLHSPFPSSEIYRTLPVREEILKALLNSDLIGFHTFDYARHFLSCCSRMLGLE YQSKRGYIGLEYYGRTVGIKIMPVGIHMGRIASVMKLADKEKK | |||
| Full Sequence |
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| Protein Sequence Length: 578 Download |
| MMSRSYTNLL DLATGNFPAI GRERKRLPRV MTVPGNVYEL DDDQANSVSS DNPSSVAQDR 60 LIIVANQLPV KANRRADDRG WVFSWNEDSL LLQLKEEEVS QILLETFKCV PTFLPHDVLE 120 KFYHGFCKKL LWPLFHYMLP FSADHGGRFD RSMWEAYVWA NKLFSQKVIE AINPDDDYVW 180 IHDYHLMVLP TFLRRHFNQL RMGFFLHSPF PSSEIYRTLP VREEILKALL NSDLIGFHTF 240 DYARHFLSCC SRMLGLEYQS KRGYIGLEYY GRTVGIKIMP VGIHMGRIAS VMKLADKEKK 300 LLAMEQLLQQ HSKWQGKTVL VQIANPARGK GADLEEIQAE IRESCRRINE EFGEPGYEPI 360 VFVDRPVSIS ERIAYYSIAE LLSEFIGCSP SLSGAICVNP WNVEATAEAM NEAISMSQSE 420 QLLRHEKHFR YVSTHDVAYW SRSFLQDMER TCSELFRRRC WGIGLKAIVS AYCRAKSRAI 480 LLDYDGTVMP QNSINKAPSQ EVISILNTLC WDKKNIVFIV SGRGRDNLSQ WFSPCRKLGL 540 AAEAILMEGI NEENERENID NLAERCVRTP RPDAFCR* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR02400 | trehalose_OtsA | 1.0e-113 | 60 | 450 | 471 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 1.0e-117 | 60 | 543 | 575 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 1.0e-151 | 60 | 449 | 471 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| pfam00982 | Glyco_transf_20 | 9.0e-172 | 60 | 450 | 476 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PLN02205 | PLN02205 | 0 | 1 | 543 | 662 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN67058.1 | 0 | 1 | 543 | 1 | 655 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI22022.1 | 0 | 1 | 543 | 1 | 582 | unnamed protein product [Vitis vinifera] |
| EMBL | CBI32507.1 | 0 | 1 | 577 | 1 | 577 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002281386.1 | 0 | 1 | 564 | 1 | 676 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002522255.1 | 0 | 1 | 543 | 1 | 657 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 1.96182e-44 | 80 | 452 | 36 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 1.96182e-44 | 80 | 452 | 36 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 1.96182e-44 | 80 | 452 | 36 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 1.96182e-44 | 80 | 452 | 36 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 1.96182e-44 | 80 | 452 | 37 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 592 | 70 | 543 | 0 |
| HO779327 | 496 | 95 | 499 | 0 |
| HO786687 | 409 | 1 | 353 | 0 |
| HO779327 | 49 | 495 | 543 | 0.0000000005 |
| HO777775 | 78 | 1 | 77 | 0.0000002 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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