| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01032411001 |
| Family | GH18 |
| Protein Properties | Length: 2500 Molecular Weight: 329254 Isoelectric Point: 3.9449 |
| Chromosome | Chromosome/Scaffold: 14 Start: 27104985 End: 27119919 |
| Description | chitinase A |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH18 | 2681 | 2895 | 3.9e-26 |
| IYWGQNSKEGTLADTCGTGRFAFVNIAFLPVFGNNQTPALDLDDHCDPSTKNGCTGLATDIRSCQSRGVKVMLSIGGGDGSYFLASSEDAKQVAAYLWDN YLGGTSPSRPFGDAVLDGIDFDIEGGTTQHWDELASYLRSYSFAKKLCLTAAPQCPFPDVYMGQALSSGLFDYVWMQFYNNYCEYKGNAAELKATWDTWT SNVTATGFFLGLPAA | |||
| Full Sequence |
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| Protein Sequence Length: 2500 Download |
| MQKINPNEQP KEECITVDEV SQLEPQENVS AIKCSDSPTE SDKAMELSEE VPEGAEKAAE 60 ERENIKELII EKERDIKEFP AVSIEDESVN KEQHSETVSD ENPRATDSEV QTENVNLEVV 120 EDFSKDPEGS PITKAMEEEM LQKQGGEKKV DVSSSVSEIS EEINKEVIED ADTCSNSIDT 180 ASEKVETEQT SLQEAQLDDK HIKIIETSSE DKTLQTNTAT ETSPQGVEVD RVKLEETSEG 240 VSQLPAIACE NRNKESEIVE KPHVDEVEEI EGASETVSES REQCVEENDG TQKSLTVEES 300 EERIIKQEIK GPSETVSGCN YEGVEAVIED EIDVVQTATE GKSEEQNQET SKALLSEERD 360 HGIIATIGYS EDGKTKEEET PQNENLEDSF ATKTADYMHL QKEEPRELEL SGLGLELNEN 420 IQKNNPNEPP KEECSTIDEV SPLEPQENVS VIKCTDSPVE SDKVMELTEE VPEGAEKAAE 480 RENIKELIIE KERDIKELPA VSTDELVIEV QVLEPVSSEN LGVTDPNMPA ENLNLEVEDS 540 SKEPIGSPIT EALEEEMSEK QDGEKAMDVL SSVSEMQEEN IKEEIMEKAD ISYSKLDAAS 600 EKGETEESSS QEAQLDDEPV KIIETSSEDK TLQTNTITET SPQAEEVDRM KLEETLELVS 660 QLPAKACENT NKESETVERP IADEIEETTG ASDTVSESKE QCAEEIDETQ KSLSQEKSEE 720 DIIKEQIKGA SEMDSESKYQ GVEAVIEEEI TAGQAIQAEK PEEQLEEVST AILSEKLEDE 780 NIKEGEIPQY KNPQDSFGTK TSENTCLQKE EPRELDVSGL GLDINKDVSK EDGNGVDGVS 840 QYELQENASE LKYTDSLLES EKVMEQTRSI NSAQQEATKD DENLDSGIFN NNPDVLTHSP 900 GEEILQEVDE NCEEASNLEC ENFEKASEPV LDLQSTEIDE AVVGLEVGAH IQGSDAPNTK 960 NSDNLIEEVP QGAEKAVESE NIKEPIIEKE RDIKELPAVY IEDETVNKVQ HAEAVSNENL 1020 RAIDSNMQTE RLNSEVAEYL SKDPEGSPIS QALQEEMSQK QASEKTVEIT ISVSEMSEES 1080 FKDEIIKEAD TCSKIDTAPE KVVTEETSLQ EVQLDDKPLK GIETSTAEKI LPTNTTTESS 1140 LQGEEVDSLK VETSELKSRL PTIACESADK ESKTVEKPHA DEVEEPKGPT DTVSESREQC 1200 VENIVETRES LTLQKSEENI IKEEIKGPSD TVSECTYQSF EAVIEDEITA DQTIEAENPE 1260 EQLQEMSTAL VSEEQEHGIS TITTKLDYEN TVEDETPQYE NQEDSLGTKM TEERCLDKEE 1320 SRELEVSELE LNDDVQKKPS NEEPKEGGNF LHGITPSEPQ EEAFEIKCRD SLAESDMELT 1380 RSMDSTSQEA SKDDEDLDSG ISIKNVDDVL PPHSSGEEIL QKVDENCEEA SNLVHSAEVD 1440 KAEIELEAGS HSHGSEASST ENNNNLTEKN IELADLGEKV GGGPDLVAED QCNETLPETN 1500 QTQVNENSSS AWEQNTKSIE EQIIGQGFQE REKETIEETE SKLESRDHIN VIQNENETTK 1560 DVISVEEVPG GAEKAEEKEN IQELMIEEKR DIKELPAVSI EDETVSKVQH SEPVSCESFR 1620 ARESNQETKE VNLEDEEDTC EKTEDVSSSV FGLSEQGVKG EIIDEADNYN KLDTASEKVE 1680 AEKTIWQEPQ LDDEPLKGIE TILEEKTPPI INTTESSPGD EEVEAVKLEK TSDLVSQLPT 1740 TAYEHADKES VIVEKAQADE VEETKRASDP VSESKDHCVE EIDETQRSLT MEKLEEDIIE 1800 EEITGRSSTV SACNQGVEAI IQDEISRESL KTGNSEEQLQ EMSTARLSEE KEHDITTTIG 1860 KSEAENKSEN ETPQKECLED SIGTKSVEEI CLQMEGLREL EVSALAPKLS NVIQKESPNE 1920 VLKEEGEPQE NVSEIKYMDS ITASEEVMKL TRSIDSSSQE ASKEEEILDS GNSNNKADDF 1980 SITHSSVEIL QQLQEQCEKT PNLESEKIEK ASESVSEVHY PEVFIKLEEP EVKEEHLKTS 2040 VTETRAEEIP HEKTVEANEI IKNEISNEEI ADEKQVAEDY QPWSLDEETI RKSQQEDELK 2100 NEVEHEELYG ESQEGETNED IEKQIIDEAR TVEHPHLVSF GDESLKDNLP EEAKGERNDE 2160 VTDIKLDLAN QSDETALSEQ VDCSDIQLVR KDLDIADTNE DKEIVITKDE DLKPNLDVLP 2220 ATSAAAEEAS LQKEGDRIGE VSESVPQEPV HETTTRNEEI ATAITDNSEN VMEDKMHTHT 2280 PLQLACNKHD VSEGLNLKDP EVDTNENGTS PLSEMSWEEI PDKEGVISDE ERQHDLTSGK 2340 QTIAESMPTE EKGAKDEEKH AKDEEEHTKD EEPRQFSARG SEGKLEEAEM HSTKCNKDGE 2400 IQDQIEKLNA SSQVQYSCME ISEKEVSMDL AEANESTQDI KQLEKGSDET SEKHIPREAE 2460 PNESTEVMIS SSKEDVQLVP QDLLRKTFQQ PEVESLEAVN 2520 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06546 | GH18_CTS3_chitinase | 4.0e-7 | 2715 | 2929 | 244 | + GH18 domain of CTS3 (chitinase 3), an uncharacterized protein from the human fungal pathogen Coccidioides posadasii. CTS3 has a chitinase-like glycosyl hydrolase family 18 (GH18) domain; and has homologs in bacteria as well as fungi. | ||
| cd00598 | GH18_chitinase-like | 9.0e-9 | 2704 | 2860 | 175 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. | ||
| cd02871 | GH18_chitinase_D-like | 8.0e-14 | 2704 | 2947 | 305 | + GH18 domain of Chitinase D (ChiD). ChiD, a chitinase found in Bacillus circulans, hydrolyzes the 1,4-beta-linkages of N-acetylglucosamine in chitin and chitodextrins. The domain architecture of ChiD includes a catalytic glycosyl hydrolase family 18 (GH18) domain, a chitin-binding domain, and a fibronectin type III domain. The chitin-binding and fibronectin type III domains are located either N-terminal or C-terminal to the catalytic domain. This family includes exochitinase Chi36 from Bacillus cereus. | ||
| pfam00704 | Glyco_hydro_18 | 7.0e-28 | 2683 | 2892 | 240 | + Glycosyl hydrolases family 18. | ||
| cd02877 | GH18_hevamine_XipI_class_III | 7.0e-125 | 2683 | 2945 | 274 | + This conserved domain family includes xylanase inhibitor Xip-I, and the class III plant chitinases such as hevamine, concanavalin B, and PPL2, all of which have a glycosyl hydrolase family 18 (GH18) domain. Hevamine is a class III endochitinase that hydrolyzes the linear polysaccharide chains of chitin and peptidoglycan and is important for defense against pathogenic bacteria and fungi. PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI34870.1 | 0 | 1 | 2966 | 1 | 2966 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002272929.1 | 0 | 1 | 865 | 1492 | 2377 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002272929.1 | 0 | 1 | 895 | 1912 | 2826 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002272929.1 | 0 | 1 | 2651 | 2332 | 4982 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002272929.1 | 0 | 993 | 1397 | 1567 | 1976 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2hvm_A | 0 | 2677 | 2943 | 1 | 269 | A Chain A, Crystal Structure Of Wild-Type E.Coli Gs In Complex With Adp And Glucose(Wtgsb) |
| PDB | 1llo_A | 0 | 2677 | 2943 | 1 | 269 | A Chain A, Crystal Structure Of Wild-Type E.Coli Gs In Complex With Adp And Glucose(Wtgsb) |
| PDB | 1hvq_A | 0 | 2677 | 2943 | 1 | 269 | A Chain A, Crystal Structures Of Hevamine, A Plant Defence Protein With Chitinase And Lysozyme Activity, And Its Complex With An Inhibitor |
| PDB | 1kr0_A | 0 | 2677 | 2943 | 1 | 269 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
| PDB | 1kr1_A | 0 | 2677 | 2943 | 1 | 269 | A Chain A, Hevamine Mutant D125aE127A IN COMPLEX WITH TETRA-Nag |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| chitin degradation II | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12623 | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12624 | EC-3.2.1.14 | chitinase |
| chitin degradation III (carnivorous plants) | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CF511598 | 284 | 1679 | 1962 | 0 |
| CF511598 | 295 | 1102 | 1391 | 0 |
| CF511598 | 286 | 604 | 878 | 0 |
| CB970594 | 279 | 2162 | 2440 | 0 |
| CF511598 | 295 | 185 | 479 | 5.99756e-43 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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