| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01033976001 |
| Family | CBM20 |
| Protein Properties | Length: 383 Molecular Weight: 42568.8 Isoelectric Point: 4.5685 |
| Chromosome | Chromosome/Scaffold: 8 Start: 16158348 End: 16161811 |
| Description | Carbohydrate-binding-like fold |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM20 | 83 | 169 | 3.4e-23 |
| VHVRLQLQKRCSYGEQFLIVGDDPMFGLWDPSSAVPLCWSDGHVWTAELDIPIGKLIQFKFILKGITGTILWQPGPDRILRTWATEN | |||
| Full Sequence |
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| Protein Sequence Length: 383 Download |
| MKALASSSSR VFPGKYREKW FSSTMDAQIR FLRCQKLVDV GFFNSISVQH KPIHPISSSS 60 PEPQAELETA ETKIQKKSRS NTVHVRLQLQ KRCSYGEQFL IVGDDPMFGL WDPSSAVPLC 120 WSDGHVWTAE LDIPIGKLIQ FKFILKGITG TILWQPGPDR ILRTWATENT ITVVEDWEDA 180 ELQKITEGPV SILNEEPVIN SDTLIAAEDV TRTKYAHLSD INKESAITVT TAHPSEKPPA 240 EAQNKLTNPG SAEIKENLIT YEGPVLVPGL TPLSTIPTEE TIQDGDKRSI SMDASSDDKQ 300 EPEGEPHHEG TTEMVFNDEK EELQDAELEQ ETSLAEENQM NSDSPGNGFL GNDVQWGRRT 360 LQKFLTNLAN SLNVLLILHI HG* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02856 | E_set_GDE_Isoamylase_N | 2.0e-14 | 82 | 177 | 100 | + N-terminal Early set domain associated with the catalytic domain of Glycogen debranching enzyme and bacterial isoamylase (also called glycogen 6-glucanohydrolase). E or "early" set domains are associated with the catalytic domain of the glycogen debranching enzyme at the N-terminal end. Glycogen debranching enzymes have both 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities. As a transferase, it transfers a segment of the 1,4-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or another 1,4-alpha-D-glucan. As a glucosidase, it catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. Bacterial isoamylases are also included in this subfamily. Isoamylase is one of the starch-debranching enzymes that catalyze the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans such as amylopectin or glycogen. Isoamylase contains a bound calcium ion, but this is not in the same position as the conserved calcium ion that has been reported in other alpha-amylase family enzymes. The N-terminal domain of glycogen debranching enzyme may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others. | ||
| smart01065 | CBM_2 | 1.0e-14 | 83 | 166 | 88 | + Starch binding domain. | ||
| cd02861 | E_set_pullulanase_like | 6.0e-15 | 85 | 172 | 90 | + Early set domain associated with the catalytic domain of pullulanase-like proteins. E or "early" set domains are associated with the catalytic domain of pullulanase at either the N-terminal or C-terminal end, and in a few instances at both ends. Pullulanase (also called dextrinase or alpha-dextrin endo-1,6-alpha glucosidase) is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. The E set domain of pullulanase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase. | ||
| pfam00686 | CBM_20 | 2.0e-17 | 82 | 174 | 96 | + Starch binding domain. | ||
| cd05467 | CBM20 | 3.0e-25 | 85 | 177 | 96 | + The family 20 carbohydrate-binding module (CBM20), also known as the starch-binding domain, is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:2001070 | starch binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN60108.1 | 0 | 25 | 369 | 1 | 426 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI30404.1 | 0 | 1 | 382 | 1 | 382 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_974720.1 | 0 | 51 | 368 | 59 | 382 | glycoside hydrolase starch-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | XP_002322974.1 | 0 | 1 | 238 | 1 | 254 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002528119.1 | 0 | 79 | 211 | 16 | 149 | catalytic, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1cyg_A | 0.000005 | 56 | 179 | 558 | 680 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 1qhp_A | 0.0001 | 96 | 164 | 597 | 672 | A Chain A, Cyclodextrin Glucanotransferase (E.C.2.4.1.19) (Cgtase) |
| PDB | 1qho_A | 0.0001 | 96 | 164 | 597 | 672 | A Chain A, Five-Domain Alpha-Amylase From Bacillus Stearothermophilus, MaltoseACARBOSE COMPLEX |
| PDB | 1ciu_A | 0.0002 | 81 | 178 | 582 | 682 | A Chain A, Five-Domain Alpha-Amylase From Bacillus Stearothermophilus, MaltoseACARBOSE COMPLEX |
| PDB | 1a47_A | 0.0002 | 81 | 178 | 582 | 682 | A Chain A, Cgtase From Thermoanaerobacterium Thermosulfurigenes Em1 In Complex With A Maltohexaose Inhibitor |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CD005961 | 199 | 1 | 199 | 0 |
| GO924256 | 232 | 29 | 254 | 0 |
| FN770891 | 220 | 28 | 241 | 0 |
| FN721308 | 214 | 1 | 202 | 0 |
| FN721082 | 212 | 15 | 214 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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