| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01035389001 |
| Family | GH32 |
| Protein Properties | Length: 540 Molecular Weight: 60858.8 Isoelectric Point: 6.9262 |
| Chromosome | Chromosome/Scaffold: 4 Start: 941254 End: 945402 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 86 | 289 | 0 |
| HFQPPKNWMNGPMYYNGVYHLFYQYNPYAAVWGNITWAHSTSYDLVNWVHLELAIKPTDPFDINGCWSGSATILTGEEPVIIYTGKDSQNRQVQNLSVPK NISDPLLREWIKSPHNPLMTPIDGIDASNFRDPTTAWQGSDKVWRILSQTPLHSSNKTGMWECPDFYPVSISSRNGVETSVQNAETRHVLKASFNGNDYY IMGK | |||
| Full Sequence |
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| Protein Sequence Length: 540 Download |
| MQKLFTLNPY SFKIHSLSFE CVVINCHRDG GKSAVKLRKE MGRFGIWVVG LCLMVGGHGI 60 EGETSHHSYR NLQSDPADQP YRTAYHFQPP KNWMNGPMYY NGVYHLFYQY NPYAAVWGNI 120 TWAHSTSYDL VNWVHLELAI KPTDPFDING CWSGSATILT GEEPVIIYTG KDSQNRQVQN 180 LSVPKNISDP LLREWIKSPH NPLMTPIDGI DASNFRDPTT AWQGSDKVWR ILSQTPLHSS 240 NKTGMWECPD FYPVSISSRN GVETSVQNAE TRHVLKASFN GNDYYIMGKY RRILWAWIQE 300 ADKDTEKGWS GLQSFPRSVL LDQNGQRLVQ WPVKEIAILH KNQVTFHNKE LRGGSVIEVS 360 GITASQADVE VSFDFPHLEE AELMDPSWTD PQALCSRKNV SVKGGIGPFG LLVLASNNLT 420 EQTAIFFRIF KSTQEKHIVL MCSDQSRSSL RQDVDKTIYG AFVDIDLNHE QISLRSLIDH 480 SIVESFGGKG KTCITARVYP ELAINTEAHL YAFNSGNQTL NISTLSAWSM KNAEMVPTN* 540 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-43 | 80 | 500 | 497 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 6.0e-53 | 77 | 500 | 493 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 4.0e-72 | 92 | 335 | 304 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-103 | 86 | 332 | 319 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-134 | 86 | 490 | 484 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAN83673.1 | 0 | 98 | 539 | 1 | 479 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI20652.1 | 0 | 1 | 539 | 1 | 539 | unnamed protein product [Vitis vinifera] |
| EMBL | CBI20653.1 | 0 | 45 | 539 | 5 | 500 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002278918.1 | 0 | 45 | 539 | 5 | 563 | PREDICTED: hypothetical protein isoform 3 [Vitis vinifera] |
| RefSeq | XP_002279133.1 | 0 | 94 | 539 | 1 | 509 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 78 | 536 | 4 | 534 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 2ade_A | 0 | 78 | 536 | 4 | 534 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 2add_A | 0 | 78 | 536 | 4 | 534 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 1st8_A | 0 | 78 | 536 | 4 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 78 | 536 | 4 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CT842376 | 557 | 65 | 535 | 0 |
| EE591202 | 416 | 198 | 535 | 0 |
| EE591202 | 126 | 82 | 202 | 0 |
| JG639929 | 266 | 46 | 280 | 0 |
| JG637950 | 261 | 46 | 275 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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