y
Basic Information | |
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Species | Glycine max |
Cazyme ID | Glyma02g43020.2 |
Family | GH85 |
Protein Properties | Length: 681 Molecular Weight: 76339 Isoelectric Point: 6.2285 |
Chromosome | Chromosome/Scaffold: 02 Start: 47900041 End: 47908728 |
Description | Glycosyl hydrolase family 85 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH85 | 70 | 354 | 0 |
MAGGYLDDKWVQGGTNAEAYAIWHWHLIDVFVYFSHNLVTLPPPSWTNTAHRHGVKVLGTFITEGDEGAVVCDEMLSTKESAQMYATKDNTQLQLNMEVS LKPKQISNLKEFVNHLSLTTHSSVPGSLVIWYDSVTINGDLWWQDELNEHNKPFFDICDGIFTNYTWQEDYPRRSAAVAGDRKFDVYMGIDIFGRNTYGG GMWNCFPDVILCMCQTNAALDVIRKDDVSAAIFAPGWVYETKQAPDFETAQNRWWSLVGKSWGITRKYLGTLPFYTNFDQGSGYH |
Full Sequence |
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Protein Sequence Length: 681 Download |
MSHSINSESE FDPKQPSVPI SYPIKTLKDL ESRSYYESFH YPFNKASVPI ESGHSAPLPN 60 RRRILVCHDM AGGYLDDKWV QGGTNAEAYA IWHWHLIDVF VYFSHNLVTL PPPSWTNTAH 120 RHGVKVLGTF ITEGDEGAVV CDEMLSTKES AQMYATKDNT QLQLNMEVSL KPKQISNLKE 180 FVNHLSLTTH SSVPGSLVIW YDSVTINGDL WWQDELNEHN KPFFDICDGI FTNYTWQEDY 240 PRRSAAVAGD RKFDVYMGID IFGRNTYGGG MWNCFPDVIL CMCQTNAALD VIRKDDVSAA 300 IFAPGWVYET KQAPDFETAQ NRWWSLVGKS WGITRKYLGT LPFYTNFDQG SGYHISVDGD 360 QVSDAPWCNI SSQGVQPLLE FADSTANSIQ LIVNLKEASY NGGGNITFKG SLEKDTYFKR 420 RIFQGEFILS KLPIHFFYSV KSDSNSSLGL VLEFTSTINK TMSVLLTSHG MDHLSSEFSK 480 VVLTSEHKGN ALGWVIHEGT IEMNGYILTG IHALCYKPNA PSKELKSRPF GPGHTAPSST 540 DYSAVLGHIT VKTSNYKPDF PISTSWLVDG EYIHWKSGPQ DSRILSVKIS WNLKGKNFAF 600 PHYNVFVEKP PKLADGNSGT TLEPVQEYLG VAHVNCFYVS ELKVPASTSS LKFIIQVCDF 660 DGTNRNLEDC PYYQLEIKGL * 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG4724 | COG4724 | 5.0e-32 | 43 | 501 | 511 | + Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism] | ||
pfam03644 | Glyco_hydro_85 | 6.0e-109 | 70 | 357 | 315 | + Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates. | ||
cd06547 | GH85_ENGase | 7.0e-118 | 65 | 382 | 346 | + Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model. |
Gene Ontology | |
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GO Term | Description |
GO:0005737 | cytoplasm |
GO:0033925 | mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_002273683.1 | 0 | 11 | 679 | 16 | 689 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002315137.1 | 0 | 17 | 679 | 26 | 698 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002315138.1 | 0 | 2 | 679 | 12 | 695 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002520781.1 | 0 | 11 | 679 | 19 | 692 | endo beta n-acetylglucosaminidase, putative [Ricinus communis] |
RefSeq | XP_002520784.1 | 0 | 11 | 679 | 17 | 686 | endo beta n-acetylglucosaminidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3fhq_F | 1e-19 | 42 | 453 | 28 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fhq_D | 1e-19 | 42 | 453 | 28 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fhq_B | 1e-19 | 42 | 453 | 28 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fhq_A | 1e-19 | 42 | 453 | 28 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
PDB | 3fha_D | 2e-19 | 42 | 453 | 28 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EV268508 | 242 | 422 | 662 | 0 |
BU550911 | 212 | 470 | 681 | 0 |
CX548533 | 234 | 449 | 681 | 0 |
CX548693 | 225 | 458 | 681 | 0 |
BF066789 | 200 | 440 | 639 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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