| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma05g29651.1 |
| Family | GT20 |
| Protein Properties | Length: 921 Molecular Weight: 104583 Isoelectric Point: 6.9962 |
| Chromosome | Chromosome/Scaffold: 05 Start: 35201618 End: 35210557 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 86 | 554 | 0 |
| SKQRLLVVANRLPVSAVREGVESYRLEISVGGLVSALLGVKEFDTRWIGWAGVNVPDDVGQRALTKALAEMRCIPVFLDEEIVNQYYNGYCNNILWPLFH YLGLPQEDRLATTRTFQSQFDAYKKANQMFADVVNKHYEKGDVVWCHDYHLMYLPKCLKEYNEQMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDHGKLTRVAAFPIGIDSDRFIQALELPEVQEHMKELKERFAGRKVMLGVDRLDMIKGIPQKLLAFEKFLEE NSLWRDKVVLLQIAVPTRKDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHELCALYAVTDVALVTSLRDGMNLVSYEFVACQASKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVAASIGYALEMSADEREKRHQFNFKHVETHTSQEWAATFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 921 Download |
| MPGNNYNCVP LTPRSRLERL LRERELRKSF KLIQPTEEAR DGSKEGEQFY GDSCISENEI 60 LGIHKEEEVT ETSFTDRSQR QDERPSKQRL LVVANRLPVS AVREGVESYR LEISVGGLVS 120 ALLGVKEFDT RWIGWAGVNV PDDVGQRALT KALAEMRCIP VFLDEEIVNQ YYNGYCNNIL 180 WPLFHYLGLP QEDRLATTRT FQSQFDAYKK ANQMFADVVN KHYEKGDVVW CHDYHLMYLP 240 KCLKEYNEQM KVGWFLHTPF PSSEIHRTLP SRSELLRSVL AADLVGFHTY DYARHFVSAC 300 TRILGLEGTP EGVEDHGKLT RVAAFPIGID SDRFIQALEL PEVQEHMKEL KERFAGRKVM 360 LGVDRLDMIK GIPQKLLAFE KFLEENSLWR DKVVLLQIAV PTRKDVPEYQ KLTSQVHEIV 420 GRINGRFGTL TAVPIHHLDR SLDFHELCAL YAVTDVALVT SLRDGMNLVS YEFVACQASK 480 KGVLILSEFA GAAQSLGAGA ILVNPWNITE VAASIGYALE MSADEREKRH QFNFKHVETH 540 TSQEWAATFV SELNDTIVEA QLRTRQVPPL LPNKVAVDCY SKSNNRLIIL GFNATLNEPV 600 GRAGQIRELE LKLHPNMKEP LKKLTDDPKT TIVVLSGSSR AVLDKNFSEF NMWLAAENGM 660 FLRRTSSEWM TTMPENLNMD WVDSVKHVFE YFTERTPRSH FELRETSVVW NYKYADVEFG 720 RIQARDLLQH LWTGPISNAY LDVVQGGRSV EVRTVGVSKG AAIDRILGEI VHSKGMKTPI 780 DYVLCIGHFL AKDEDVYTFF EPELPSESPP LPRAMLSKSN SYRPSSLPKL PATKTGSKAA 840 KYRKQRSLSN IEKREIDQWR PKYRDKIALH EGSSVLDLNG DNYFSCVVGR KRSSARYLLK 900 TSDDVVNLLS DLAEHSSSPT * 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 918 | 934 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 82 | 920 | 839 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 89 | 555 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 88 | 555 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 89 | 787 | 706 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_177979.1 | 0 | 1 | 919 | 1 | 942 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002305707.1 | 0 | 21 | 920 | 1 | 899 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002331300.1 | 0 | 1 | 917 | 1 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522296.1 | 0 | 1 | 917 | 1 | 913 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| RefSeq | XP_002531237.1 | 0 | 1 | 917 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 89 | 565 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 89 | 565 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DW069910 | 288 | 175 | 462 | 0 |
| CV711307 | 294 | 86 | 379 | 0 |
| CX546763 | 301 | 79 | 378 | 0 |
| ES813375 | 355 | 450 | 800 | 0 |
| CB651133 | 280 | 116 | 395 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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