| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma07g01090.1 |
| Family | GH32 |
| Protein Properties | Length: 565 Molecular Weight: 63520.2 Isoelectric Point: 9.0064 |
| Chromosome | Chromosome/Scaffold: 07 Start: 642802 End: 647826 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 45 | 362 | 0 |
| HFQPPKNWINDPNGPLRYAGLYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDPAIFPSQPSDINGCWSGSTTLLPGNKPVILYTGIDLLNQQVQNLA QPKNLSDPFLREWVKSPKNPLMAPTSANKINSSSFRDPTTAWLGKDGHWRVLVGSKRRTRGIAILYRSKDFVNWVQAKHPLYSILGSGMWECPDFFPVLN NDQLGVDTSVNGYDVRHVLKVSLDDKKHDYYMIGSYNAAKDAFIPDEESNEFVLRYDYGKYYASKTFFDDGKKRRILLGWANESSSVAADIKKGWSGIHT IPRALWLHKSGKQLVQWP | |||
| Full Sequence |
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| Protein Sequence Length: 565 Download |
| MAMSTILLLT FFSFIYGSAA THHVYRNLQS LSSDSSNQPY RTAYHFQPPK NWINDPNGPL 60 RYAGLYHLFY QYNPKGAVWG NIVWAHSVSK DLVNWTPLDP AIFPSQPSDI NGCWSGSTTL 120 LPGNKPVILY TGIDLLNQQV QNLAQPKNLS DPFLREWVKS PKNPLMAPTS ANKINSSSFR 180 DPTTAWLGKD GHWRVLVGSK RRTRGIAILY RSKDFVNWVQ AKHPLYSILG SGMWECPDFF 240 PVLNNDQLGV DTSVNGYDVR HVLKVSLDDK KHDYYMIGSY NAAKDAFIPD EESNEFVLRY 300 DYGKYYASKT FFDDGKKRRI LLGWANESSS VAADIKKGWS GIHTIPRALW LHKSGKQLVQ 360 WPVVEVEKLR AYPVNLPPQV LKGGKLLPIN GVTATQADVE ISFEVSNLRE AEVLDYWTDP 420 QILCSKKGSS IKSGLGPFGL LVFASEGLQE YTSVFFRIFR HQHKYLVLLC SDQSRSSLNK 480 DNDLTSYGTF VDVDPLHEKL SLRTLIDHSV VESFGGEGRA CITARVYPTL AINDEAQLYA 540 FNNGTADVNI TKLNAWSMKK AQIN* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 1.0e-56 | 39 | 528 | 511 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 3.0e-74 | 39 | 552 | 525 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 9.0e-93 | 51 | 365 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-149 | 45 | 362 | 326 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-180 | 45 | 518 | 484 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAC17166.1 | 0 | 20 | 562 | 22 | 573 | cell wall invertase [Pisum sativum] |
| GenBank | ABD28503.1 | 0 | 20 | 564 | 25 | 572 | Sialidase [Medicago truncatula] |
| EMBL | CAA84527.1 | 0 | 20 | 564 | 26 | 578 | cell wall invertase II; beta-furanofructosidase [Vicia faba var. minor] |
| EMBL | CAD91338.1 | 0 | 1 | 564 | 1 | 564 | beta-fructofuranosidase [Glycine max] |
| Swiss-Prot | Q43089 | 0 | 20 | 538 | 22 | 549 | INV1_PEA RecName: Full=Beta-fructofuranosidase, cell wall isozyme; AltName: Full=Sucrose hydrolase; AltName: Full=Acid invertase; Flags: Precursor |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 33 | 564 | 1 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 37 | 564 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 37 | 564 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 37 | 564 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 37 | 564 | 1 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
