| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma08g26861.3 |
| Family | GH13 |
| Protein Properties | Length: 958 Molecular Weight: 108756 Isoelectric Point: 5.5384 |
| Chromosome | Chromosome/Scaffold: 08 Start: 21187067 End: 21198701 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 589 | 872 | 1.6e-37 |
| LSNCGVTAVWLPPPTESVAPQGYMPSDLYNLNSSYGSVEELKYCIEEMHSQDLLALGDVVLNHRCAQKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQGRG NPSSGDIFHAAPNVDHSQDFVRKDIKEWLNWLRNDIGFDGWRLDFVRGFSGTYVKEYIEASTPVFAIGEYWDSLGYEHGSLCYNQDAHRQRIINWINATG GTSSAFDMTTKGILHSALHNEYWRLIDPQGKPTGVMGWWASRAVTFLENHDTGSTQGHWPFPRDKLMQGYAYILTHPGTPTIFY | |||
| Full Sequence |
|---|
| Protein Sequence Length: 958 Download |
| MGAALLPDAA FGIFPRFFNV PRHPLICRTT SATSVRKRNF FFGDKRISKS SLIVLSHLND 60 SDDKLTDVVV DQDDVIGIED ELVVTKKALS EAQHRQEIIE KERDQLLEEL ARSEAKKQEY 120 ITTILHDKEV AISELEAAKT LFQKKLEDSV EEKFNLESKL VLAKQDAVDL AVQVEKLAEV 180 AFQQATSHIL EDAQLRISSA ETTAAEAAHL IENQIKDAIE GTISSIVEKS NHAIERALVV 240 AEKAEELAKK STETFIDGTS PFTEIAAVEA ENIKLQGIII DIESELMMAR SQADKLKLEL 300 ENTRQQLQAF EQRANDAEKA LLDFQESSRE NILQREEEMK SMLEKVKKDV ADRTKAISKA 360 FKADLKNIKA TVEAAKEVVH CKDYAYLRRC EALQRSLKSS EDAVKMWRQR AEMAESLLLK 420 ERLQDEGDAD SIYVVNGGRI DLLTDVDSQK WKLLSDGPRR EIPQWMARRI NAVSPKFPPK 480 KIDVAEALTS KFRSLELPTV DEVWSIAREK PKEGDALIEH VYERETIEKK RKALERALHR 540 KTIQWQRAPE QTKLEPGTGT GREIVFQGFN WESWRRRWYL ELAAKTADLS NCGVTAVWLP 600 PPTESVAPQG YMPSDLYNLN SSYGSVEELK YCIEEMHSQD LLALGDVVLN HRCAQKQSPN 660 GVWNIFGGKL AWGPEAIVCD DPNFQGRGNP SSGDIFHAAP NVDHSQDFVR KDIKEWLNWL 720 RNDIGFDGWR LDFVRGFSGT YVKEYIEAST PVFAIGEYWD SLGYEHGSLC YNQDAHRQRI 780 INWINATGGT SSAFDMTTKG ILHSALHNEY WRLIDPQGKP TGVMGWWASR AVTFLENHDT 840 GSTQGHWPFP RDKLMQGYAY ILTHPGTPTI FYDHFYDFGI HDVLTELIDA RRRAGIHCRS 900 SIKIYHANNE GYVAQVGDAL VMKLGQFDWN PSKENQLEGS WQKFVDKGPD YQVWLRQ* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 5.0e-45 | 563 | 926 | 459 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 7.0e-145 | 563 | 954 | 406 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-166 | 564 | 902 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02784 | PLN02784 | 0 | 551 | 954 | 406 | + alpha-amylase | ||
| PLN02361 | PLN02361 | 0 | 561 | 954 | 399 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI21221.1 | 0 | 1 | 957 | 1 | 975 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_001782830.1 | 0 | 565 | 956 | 14 | 404 | predicted protein [Physcomitrella patens subsp. patens] |
| RefSeq | XP_002276872.1 | 0 | 1 | 950 | 1 | 968 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002324108.1 | 0 | 566 | 957 | 10 | 401 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526120.1 | 0 | 1 | 957 | 1 | 972 | alpha-amylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3bsg_A | 0 | 562 | 926 | 1 | 378 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 2qps_A | 0 | 562 | 926 | 1 | 378 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1rpk_A | 0 | 562 | 926 | 1 | 378 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1p6w_A | 0 | 562 | 926 | 1 | 378 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1ht6_A | 0 | 562 | 926 | 1 | 378 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
| starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO795567 | 639 | 320 | 958 | 0 |
| HO778903 | 512 | 76 | 587 | 0 |
| HO778903 | 105 | 585 | 689 | 0 |
| HO785297 | 258 | 568 | 825 | 0 |
| HO785297 | 103 | 845 | 947 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
