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Basic Information | |
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Species | Glycine max |
Cazyme ID | Glyma09g03250.1 |
Family | GH31 |
Protein Properties | Length: 936 Molecular Weight: 104490 Isoelectric Point: 7.0495 |
Chromosome | Chromosome/Scaffold: 09 Start: 2286385 End: 2290726 |
Description | Glycosyl hydrolases family 31 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 284 | 751 | 0 |
YFFAGSSPELVLEQYTQLIGRPAPMPYWSFGFHQCRWGYKNVSDLEDVVANYAKAGIPLEVMWTDIDYMDAFKDFTLDPINFPLDKMRSFVDTLHKNGQK YVLILDPGISVNETYATYDRGLKADVYIKRNGNNYLGQVWPGPVYYPDFLNPRSQAFWGGEIKLFRDLLPIDGIWLDMNELSNFITSPPIPSSNLDNPPY KVNNVGDQRPINDKTVPATSLHFGNITEYNVHNLYGLLESKVTNKALKDITGKRPFILSRSTFVSSGKYAAHWTGDNAATWNDLAYSIPAILNSGIFGIP MVGADICGFEGNTTEELCGRWIQLGAFYPFARDHSVINSIRQELYVWDSVASSARKVLGLRYRLLPYFYTLMYEAHTKGTPIARPLFFSFPEDVTTYEIN SQFLLGRGVLVSPVLQSGATTVDAYFPKGTWFDLFNVSNSVNAESGKYVTLDAPYDHINVHVGEGNIL |
Full Sequence |
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Protein Sequence Length: 936 Download |
MDTTTTIAPL LFCTCACLIF FSASLSEATV VGYGYTISTV NNYPIKNSLT ANLNLIKSSS 60 VSGPDIPHLS LTASFENKDR LRVRITDSNH QRWEIPQEVI PRDSSSQHYP LGFLNTKQGS 120 HQPKDSLSLT HSDSDLVFSL HNTTPFGFTV SRKSSNDVLF HAAPDPSNPE TFLVFKDQYL 180 QLSSSLPSQR ASLYGFGEHT KSSFKLRPNQ TLTLWNADIA SANLDLNLYG SHPFYLDVRS 240 HSSDGKVKAG TTHGVLLLNS NGMDIVYGGD RITYKVIGGV FDLYFFAGSS PELVLEQYTQ 300 LIGRPAPMPY WSFGFHQCRW GYKNVSDLED VVANYAKAGI PLEVMWTDID YMDAFKDFTL 360 DPINFPLDKM RSFVDTLHKN GQKYVLILDP GISVNETYAT YDRGLKADVY IKRNGNNYLG 420 QVWPGPVYYP DFLNPRSQAF WGGEIKLFRD LLPIDGIWLD MNELSNFITS PPIPSSNLDN 480 PPYKVNNVGD QRPINDKTVP ATSLHFGNIT EYNVHNLYGL LESKVTNKAL KDITGKRPFI 540 LSRSTFVSSG KYAAHWTGDN AATWNDLAYS IPAILNSGIF GIPMVGADIC GFEGNTTEEL 600 CGRWIQLGAF YPFARDHSVI NSIRQELYVW DSVASSARKV LGLRYRLLPY FYTLMYEAHT 660 KGTPIARPLF FSFPEDVTTY EINSQFLLGR GVLVSPVLQS GATTVDAYFP KGTWFDLFNV 720 SNSVNAESGK YVTLDAPYDH INVHVGEGNI LALQGEAMTT DAARKTAFQL VVVISSSRSS 780 YGQLYLDDGE ALDMAGAKDQ WTLVSFYGAL HNNSVSVTSK VTNGRFALDQ RWILDKVTFL 840 RIPKLAGNEL SIYCWLLIIY AIKLAVTFIF GSLRSYLCKL QHNSSHSILS SMQASNAVFT 900 KPYGFIISYS LEHPRMTVFF PSKNYIRISY ARNIS* 960 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06603 | GH31_GANC_GANAB_alpha | 2.0e-111 | 303 | 662 | 367 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
cd06604 | GH31_glucosidase_II_MalA | 2.0e-130 | 303 | 662 | 366 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
COG1501 | COG1501 | 3.0e-133 | 194 | 790 | 609 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
pfam01055 | Glyco_hydro_31 | 0 | 284 | 751 | 472 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 0 | 303 | 680 | 383 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI39013.1 | 0 | 10 | 844 | 902 | 1709 | unnamed protein product [Vitis vinifera] |
EMBL | CBI39013.1 | 0 | 10 | 850 | 19 | 821 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002298949.1 | 0 | 10 | 848 | 26 | 862 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317678.1 | 0 | 27 | 847 | 43 | 861 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317679.1 | 0 | 10 | 847 | 28 | 853 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 3 | 844 | 13 | 853 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 3w37_A | 0 | 3 | 844 | 13 | 853 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 3ctt_A | 0 | 49 | 798 | 65 | 782 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 2qmj_A | 0 | 49 | 798 | 65 | 782 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 |
PDB | 2qly_A | 0 | 49 | 798 | 65 | 782 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |