Basic Information | |
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Species | Glycine max |
Cazyme ID | Glyma09g36580.2 |
Family | GH32 |
Protein Properties | Length: 631 Molecular Weight: 70591 Isoelectric Point: 5.3556 |
Chromosome | Chromosome/Scaffold: 09 Start: 42282572 End: 42287649 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 106 | 424 | 0 |
HFQPQRNWMNDPNGPLFYMGWYHIFYQYNPDSAVWGNITWGHAVSRDLIHWLYLPIALVPDKWFDISGVWSGSATLLPDGKILMLYTGNTDRNVQVQNLA YPANLSDPLLLDWVKYANNPVLVPPPGIGPKDFRDPTTAWIGPDEKWRITIGSKLNKTGLSLLYKTQDFIHYEQSDRYLHQVPGTGMWECVDFYPVSVNG PNGLDTSENGPDVKHVLKASLDDTKVDHYAIGTYFIENDTWVPDNPNEDVGIGLKLDYGRYYASKTFYDQQKQRRILWGWINESDSETADLKKGWASLQT IPRTVVFDKKTRTNLLHWP |
Full Sequence |
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Protein Sequence Length: 631 Download |
MAAMNPDLEH APQIPLLNPP TGESGRRTQK GTLVFIVSIV FLLSFIIINL QSHEPSFENN 60 ITTVPLLPIA RGVAEGVSAK SNPYLSQKAS YNWTNAMLSW QRTAFHFQPQ RNWMNDPNGP 120 LFYMGWYHIF YQYNPDSAVW GNITWGHAVS RDLIHWLYLP IALVPDKWFD ISGVWSGSAT 180 LLPDGKILML YTGNTDRNVQ VQNLAYPANL SDPLLLDWVK YANNPVLVPP PGIGPKDFRD 240 PTTAWIGPDE KWRITIGSKL NKTGLSLLYK TQDFIHYEQS DRYLHQVPGT GMWECVDFYP 300 VSVNGPNGLD TSENGPDVKH VLKASLDDTK VDHYAIGTYF IENDTWVPDN PNEDVGIGLK 360 LDYGRYYASK TFYDQQKQRR ILWGWINESD SETADLKKGW ASLQTIPRTV VFDKKTRTNL 420 LHWPVEEVES LRLSNSEFEG VVVKPGSVVP LDIGPATQLD IFAEFEIEDL ASKGIGKDNV 480 DCGNGAVDRS AFGPFGILAI ADDQLSELTP IYFHLSSTTK DGSLTTSFCV DETRSSKAPD 540 VSKLIFGSKA PVLSDEKLSM RVLVDHSIIE SFAQGGRTVI TSRVYPTEAI YGAARLFLFN 600 NATDINIKAS LKIWQLNSAF IRPFPFDQKL * |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-53 | 102 | 586 | 502 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-70 | 102 | 588 | 503 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 4.0e-95 | 112 | 427 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-151 | 106 | 424 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 106 | 576 | 481 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACJ04702.1 | 0 | 6 | 624 | 17 | 629 | invertase 2 [Cucumis melo] |
DDBJ | BAF34363.1 | 0 | 68 | 628 | 82 | 641 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35858.1 | 0 | 69 | 628 | 86 | 644 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 69 | 628 | 86 | 644 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 9 | 628 | 10 | 638 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 91 | 624 | 8 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
PDB | 3ugh_A | 0 | 91 | 624 | 8 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
PDB | 3ugg_B | 0 | 91 | 624 | 8 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
PDB | 3ugg_A | 0 | 91 | 624 | 8 | 544 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
PDB | 3ugf_B | 0 | 91 | 624 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |