| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma15g18560.1 |
| Family | AA7 |
| Protein Properties | Length: 544 Molecular Weight: 61546.2 Isoelectric Point: 8.8543 |
| Chromosome | Chromosome/Scaffold: 15 Start: 15385973 End: 15390454 |
| Description | cytokinin oxidase 3 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 70 | 276 | 2.3e-24 |
| YGNLVREVPLAVFHPASASDIARLIKLSYNGSVPFKIAARGQGHSTRGQAMAREGVVVDMAGFRERGNGVGIRVVSSVDPNNKNGYYYYADVGGEQLWID VLHATLEHGLAPMSWTDYLYLTLGGTLSNAGISGQTFRYGPQITTVREMDVITGKGEFVTCSQQTNSELFHAVLGGLGQFGIITRARIALAPAPKRVKWV RLLYNDF | |||
| Full Sequence |
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| Protein Sequence Length: 544 Download |
| MVAENYPSPT YFILLFITIT RLISTVGKTS QWTKALSLTP ELASVSLDDT IFCKLRDDPE 60 ALQGRASRDY GNLVREVPLA VFHPASASDI ARLIKLSYNG SVPFKIAARG QGHSTRGQAM 120 AREGVVVDMA GFRERGNGVG IRVVSSVDPN NKNGYYYYAD VGGEQLWIDV LHATLEHGLA 180 PMSWTDYLYL TLGGTLSNAG ISGQTFRYGP QITTVREMDV ITGKGEFVTC SQQTNSELFH 240 AVLGGLGQFG IITRARIALA PAPKRVKWVR LLYNDFSAFT KDQEQLISVT GRKQNVSLDY 300 LEGLLLMHQG PINNWRSSFF PLADHARIIS LVTKHSVLYC LEVAKYYDGQ NENNVDKELQ 360 VLLQGLSYIP GFYYEKDVSY FEFLNRVRSG ELKLQSQGLW DVPHPWLNLF IPKSQIMEFD 420 SGVFKNIILK RNITTGPVLV YPMNRNKWDN RMSASIPDED IFYTVGFLHS SGFDNWKAYD 480 AQNKEILQFC NVAGIKVKQY LPHYRTQEDW ANHFGPKWRT FVERKHQFDP RMILSPGQRI 540 FNN* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK11230 | PRK11230 | 1.0e-5 | 75 | 267 | 202 | + glycolate oxidase subunit GlcD; Provisional | ||
| pfam01565 | FAD_binding_4 | 3.0e-20 | 78 | 231 | 155 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 2.0e-24 | 54 | 538 | 499 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 1.0e-149 | 263 | 541 | 284 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 36 | 543 | 511 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009690 | cytokinin metabolic process |
| GO:0016491 | oxidoreductase activity |
| GO:0019139 | cytokinin dehydrogenase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABN05760.1 | 0 | 2 | 543 | 1 | 539 | FAD linked oxidase, N-terminal [Medicago truncatula] |
| GenBank | ACM79256.1 | 0 | 9 | 543 | 8 | 525 | cytokinin oxidase/dehydrogenase [Gossypium hirsutum] |
| RefSeq | XP_002279519.1 | 0 | 2 | 543 | 1 | 520 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002308300.1 | 0 | 2 | 543 | 1 | 528 | cytokinin oxidase [Populus trichocarpa] |
| RefSeq | XP_002514119.1 | 0 | 2 | 542 | 1 | 529 | gulonolactone oxidase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3s1d_A | 0 | 41 | 542 | 19 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 41 | 542 | 19 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 41 | 542 | 19 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3c0p_A | 0 | 41 | 542 | 19 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3bw7_A | 0 | 41 | 542 | 19 | 516 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CO121610 | 268 | 197 | 464 | 0 |
| CO979507 | 197 | 321 | 517 | 0 |
| HO781924 | 486 | 66 | 541 | 0 |
| DV135604 | 253 | 290 | 542 | 0 |
| HO792040 | 180 | 364 | 543 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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