| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma15g25022.1 |
| Family | GH18 |
| Protein Properties | Length: 763 Molecular Weight: 86075.2 Isoelectric Point: 6.8404 |
| Chromosome | Chromosome/Scaffold: 15 Start: 26166488 End: 26172502 |
| Description | S-locus lectin protein kinase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH18 | 31 | 354 | 0 |
| AGYWYSGSGFPVSDINSALYTHLICAFAELNSSTYELYVSPEDEQSFSSFTTTVKQKNPSITTLLSIAGGNGNDTVLSLMVSKDSSRKYFIQSSIRIARL YGFQGLDLSWVPETISDMNNMGRLFEEWRAAAKSEAANDSTQVLILTAAVHFRPGLDSASYPVESIQNNLNWVHILTYDYHMPQLANFTAAHAALYDPSS SVNTDNGIKEWIGSGVTASKLVLGLPFYGYAWNLRNPEDNAIGASATGPAIGKSGAMNYKDIKAYIQRYGGHVKYNATYVVNYFSNGSTWIGYDDVEVVK MKVSYARENKLLGYAVWQVPYDDN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 763 Download |
| MVSRITIALV LFEFLLCQEF EPLKAQTWLQ AGYWYSGSGF PVSDINSALY THLICAFAEL 60 NSSTYELYVS PEDEQSFSSF TTTVKQKNPS ITTLLSIAGG NGNDTVLSLM VSKDSSRKYF 120 IQSSIRIARL YGFQGLDLSW VPETISDMNN MGRLFEEWRA AAKSEAANDS TQVLILTAAV 180 HFRPGLDSAS YPVESIQNNL NWVHILTYDY HMPQLANFTA AHAALYDPSS SVNTDNGIKE 240 WIGSGVTASK LVLGLPFYGY AWNLRNPEDN AIGASATGPA IGKSGAMNYK DIKAYIQRYG 300 GHVKYNATYV VNYFSNGSTW IGYDDVEVVK MKVSYARENK LLGYAVWQVP YDDNWVLSSA 360 AAEHVDQNGR NSWRLLVIIL IITAMSVILL GILIYYLRRR FPKSTAVILS TLNNVNKDAS 420 RLFHSNAPDL QVFSFSDIEQ ATNRFSIENK VGQGGYGPVY KGILSNRQEV AVKKLSKAST 480 QGFEEFKNEV MLTARLQHVN LVRLLGFYID GEQQMLVYEY MPNKSLDSYL FDPIRRYLLD 540 WRKRIYIIEG ITQGLLYLQE YSRLTIIHRD IKASNILLDN EMKPKISDFG MARIFRKDEL 600 EANTSKIVGT YGYVSPEYAM KGLYSTKSDV YSFGVLLLQI VSGRRTACFY GEHENLNLME 660 YAYELWKEGK GMEFADPSLD DSHSTCKLLR CMQIALLCVQ EDANDRPTVK EISSMLKSDT 720 ILIIPQKPAF SINRDEKKPN KFIMHEEKCS INDATISQVV AR* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart00221 | STYKc | 3.0e-51 | 446 | 716 | 278 | + Protein kinase; unclassified specificity. Phosphotransferases. The specificity of this class of kinases can not be predicted. Possible dual-specificity Ser/Thr/Tyr kinase. | ||
| cd02872 | GH18_chitolectin_chitotriosidase | 2.0e-61 | 40 | 353 | 338 | + This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens. | ||
| pfam00704 | Glyco_hydro_18 | 3.0e-71 | 31 | 352 | 332 | + Glycosyl hydrolases family 18. | ||
| smart00636 | Glyco_18 | 1.0e-73 | 31 | 352 | 340 | + Glyco_18 domain. | ||
| cd02879 | GH18_plant_chitinase_class_V | 2.0e-132 | 26 | 358 | 339 | + The class V plant chitinases have a glycosyl hydrolase family 18 (GH18) domain, but lack the chitin-binding domain present in other GH18 enzymes. The GH18 domain of the class V chitinases has endochitinase activity in some cases and no catalytic activity in others. Included in this family is a lectin found in black locust (Robinia pseudoacacia) bark, which binds chitin but lacks chitinase activity. Also included is a chitinase-related receptor-like kinase (CHRK1) from tobacco (Nicotiana tabacum), with an N-terminal GH18 domain and a C-terminal kinase domain, which is thought to be part of a plant signaling pathway. The GH18 domain of CHRK1 is expressed extracellularly where it binds chitin but lacks chitinase activity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0004672 | protein kinase activity |
| GO:0005524 | ATP binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006468 | protein phosphorylation |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI35714.1 | 0 | 7 | 762 | 401 | 1130 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002263709.1 | 0 | 25 | 762 | 22 | 764 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002263792.1 | 0 | 7 | 762 | 6 | 753 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002270006.1 | 0 | 25 | 762 | 22 | 765 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002324558.1 | 0 | 1 | 762 | 1 | 763 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3alg_A | 0 | 29 | 363 | 4 | 343 | A Chain A, Crystal Structure Of Class V Chitinase (E115q Mutant) From Nicotiana Tobaccum In Complex With Nag4 |
| PDB | 3alf_A | 0 | 29 | 363 | 4 | 343 | A Chain A, Crystal Structure Of Class V Chitinase From Nicotiana Tobaccum |
| PDB | 3aqu_D | 0 | 26 | 366 | 2 | 344 | A Chain A, Crystal Structure Of A Class V Chitinase From Arabidopsis Thaliana |
| PDB | 3aqu_C | 0 | 26 | 366 | 2 | 344 | A Chain A, Crystal Structure Of A Class V Chitinase From Arabidopsis Thaliana |
| PDB | 3aqu_B | 0 | 26 | 366 | 2 | 344 | A Chain A, Crystal Structure Of A Class V Chitinase From Arabidopsis Thaliana |
