| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma15g27480.4 |
| Family | GT20 |
| Protein Properties | Length: 916 Molecular Weight: 103222 Isoelectric Point: 7.4525 |
| Chromosome | Chromosome/Scaffold: 15 Start: 29701522 End: 29716647 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 75 | 542 | 0 |
| RQRLLVVANRLPVSAVRKGEDAWSLEMSAGGLVSALLGVKEFEAKWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFHY LGLPQEDRLATTRSFQSQFEAYEKANQMFADVVNRHYEEGDVVWCHDYHLMFLPKCLKTHNKKMKVGWFLHTPFPSSEIHRTLPSRSELLHSVLAADLVG FHTYDYARHFVSACTRILGLEGTPYGVEYQGKLTRVAAFPIGIDSERFIRALDLPPVQDHIKELQERFKGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN AYWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQEKKKGVLIL SEFAGAAQSLGAGAILVNPWNITEVAAAIARALNMPSAEREKRHKHNFNHVISHTAQEWAGTFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 916 Download |
| MPGNKYNGNS SNIPTSRLER LLRDRELRKS GRASENNGEA MEEEVVRLRE EESAAEAATK 60 GAGGGEERQE TKPFRQRLLV VANRLPVSAV RKGEDAWSLE MSAGGLVSAL LGVKEFEAKW 120 IGWAGVNVPD EIGQKALTKA LAEKRCIPVF LDEEIVHQYY NGYCNNILWP LFHYLGLPQE 180 DRLATTRSFQ SQFEAYEKAN QMFADVVNRH YEEGDVVWCH DYHLMFLPKC LKTHNKKMKV 240 GWFLHTPFPS SEIHRTLPSR SELLHSVLAA DLVGFHTYDY ARHFVSACTR ILGLEGTPYG 300 VEYQGKLTRV AAFPIGIDSE RFIRALDLPP VQDHIKELQE RFKGRKVMLG VDRLDMIKGI 360 PQKILAFEKF LEENAYWRDK VVLLQIAVPT RTDVPEYQKL TSQVHEIVGR INGRFGTLTA 420 VPIHHLDRSL DFHALCALYA VTDVALVTSL RDGMNLVSYE FVACQEKKKG VLILSEFAGA 480 AQSLGAGAIL VNPWNITEVA AAIARALNMP SAEREKRHKH NFNHVISHTA QEWAGTFVSE 540 LNDTVIEAQL RTRQVPPRLP TKTAIESYQQ STNRLLILGF SGTLTEPVEK TGDQIKEMEL 600 KVHPKLRQPL TALCSDPNTT VVVLSGSGRQ VLDDNFKEYD MWLAAENGMF LHPSKGEWMT 660 TMPEHLNMEW VDSVKHVFEY FTERTPRSHF VFEERETSLV WNYKYSDVEF GKLQARDMLQ 720 HLWTGPISNA SVEVVQGSRS VEVRAVGVTK GAAIDRILGE IVHSKSMTSP IDYVLCIGHF 780 LGKDEDLYSF FEPDLPSIGV GLPRSKVTTD GVKFPVERKP SLNVPATKSG PKSFPNKAQR 840 SVSNSEKKTN NHICSTPRRP APEKESWNVL DLKKENYFSC AIGRTRTNAR YILDSSDDVV 900 SFLKELAVAS SRLYH* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 911 | 934 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 75 | 911 | 837 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 77 | 543 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 76 | 543 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 77 | 778 | 709 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 1 | 909 | 1 | 919 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| RefSeq | NP_177979.1 | 0 | 1 | 911 | 1 | 940 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002285632.1 | 0 | 1 | 911 | 1 | 925 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 911 | 1 | 922 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 911 | 1 | 924 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 77 | 553 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 77 | 553 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 77 | 553 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 77 | 553 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 77 | 553 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CV711307 | 293 | 75 | 367 | 0 |
| CX546763 | 292 | 75 | 366 | 0 |
| DW069910 | 288 | 163 | 450 | 0 |
| ES813375 | 402 | 438 | 835 | 0 |
| GO265628 | 302 | 432 | 733 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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