| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma17g14750.2 |
| Family | GH32 |
| Protein Properties | Length: 482 Molecular Weight: 54013.8 Isoelectric Point: 5.0111 |
| Chromosome | Chromosome/Scaffold: 17 Start: 11502335 End: 11506188 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| GH32 | 118 | 436 | 0 |
| HFQPEKNWMNDPNGPMYYKGWYHFFYQYNPNGAVWGDIVWGHAVSRDMIHWLHLPLAMVADQWYDMQGVWTGSATILPNGEIIMLYTGSTNESVQVQNLA YPADPSDPLLVDWIKYPGNPVLVPPPGIGTKDFRDPTTAWLTSEGKWRITIGSKLNKTGIALVYDTEDFKSYELKEGLLRAVDGTGMWECVDFFPVSKKN ENGLDTSVNGDEVKHVMKVSLDDDRHDYYAIGTYDEKSVLFTPDDAKNDVGVGLRYDYGIFYASKTFYDQNKERRLLWGWIGESDSEYADVAKGWASVQS IPRTVELDRKTGSNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 482 Download |
| MDHRKPLLPT SSDDAPNPRT RKDLVLMICG LFLLSSLVAF GGYRASNAPH ADVSSPASND 60 EQPSPTSVPS PKWYPVSRGV SSGVSEKSSS MLFAVKDGAS EAFPWDNSML SWQRTAFHFQ 120 PEKNWMNDPN GPMYYKGWYH FFYQYNPNGA VWGDIVWGHA VSRDMIHWLH LPLAMVADQW 180 YDMQGVWTGS ATILPNGEII MLYTGSTNES VQVQNLAYPA DPSDPLLVDW IKYPGNPVLV 240 PPPGIGTKDF RDPTTAWLTS EGKWRITIGS KLNKTGIALV YDTEDFKSYE LKEGLLRAVD 300 GTGMWECVDF FPVSKKNENG LDTSVNGDEV KHVMKVSLDD DRHDYYAIGT YDEKSVLFTP 360 DDAKNDVGVG LRYDYGIFYA SKTFYDQNKE RRLLWGWIGE SDSEYADVAK GWASVQSIPR 420 TVELDRKTGS NLLQWPVAEV ESLRLRSDEF KNLKAKPGSV VSIDIETATQ VCVHDIKFLK 480 L* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-56 | 114 | 444 | 352 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 6.0e-73 | 114 | 471 | 372 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-101 | 124 | 439 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-154 | 118 | 436 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 7.0e-165 | 118 | 470 | 360 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM52062.1 | 0 | 41 | 471 | 43 | 469 | vacuolar acid invertase PsI-1 [Pisum sativum] |
| GenBank | ACT21538.1 | 0 | 69 | 473 | 1 | 405 | acid invertase [Vigna radiata] |
| Swiss-Prot | O24509 | 0 | 1 | 473 | 1 | 477 | INVA_PHAVU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Flags: Precursor |
| Swiss-Prot | P29001 | 0 | 1 | 473 | 1 | 475 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
| Swiss-Prot | Q43857 | 0 | 49 | 473 | 46 | 467 | INVA_VICFA RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Flags: Precursor |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 103 | 471 | 8 | 376 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugh_A | 0 | 103 | 471 | 8 | 376 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugg_B | 0 | 103 | 471 | 8 | 376 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugg_A | 0 | 103 | 471 | 8 | 376 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugf_B | 0 | 103 | 471 | 8 | 376 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
