| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma18g10385.1 |
| Family | GH13 |
| Protein Properties | Length: 900 Molecular Weight: 103493 Isoelectric Point: 6.4882 |
| Chromosome | Chromosome/Scaffold: 18 Start: 9243255 End: 9260264 |
| Description | Alpha amylase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 416 | 688 | 1.2e-25 |
| FNDFTDKVLPYIKEAGYNAIQLIGIVEHKDYFTVGYRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLLIILEIVHSYAAADEMVGLSMFDGSNDCFFRSGK RGQHKFWGTRMFKYGDPDVLHFLLSNLNWWIVEYQIDGFQFHSVSSMMYTHNGFASFTGELEEYCNQYVDKDALVYLILANEILHSLHPNIITIAEDATF YPGLCEPTSQGGLGFDYYVNLSVPDMWSTFLESVPDHEWSMTKIVNTLVSNREHADKMLMYAENHNQSISGRR | |||
| Full Sequence |
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| Protein Sequence Length: 900 Download |
| MSSTLSIPLG FCFPPTVATS FPHSQTKPQN VTFQRRKTTT KACAASENPN QRQNPKQNQK 60 EAKTKNAGDD DGEKGINPAG FLAKRGISHK AFAQFLRERY KVLKDMKDEI LKRHENFMIL 120 ASGFELLGMH RHPEHRVDYM EWAPGARYCA IIGDFNGWSP TEDCAREHYF GHDDFGYWFI 180 ILQDKLREGE EPDKYYFQMY NYVDDYDKGD SGVSVEELIK KANEKYWQPG EDRFVNNRFE 240 GPVKLYEQIF GPNGPQTIED IPDIPDPETR YKAWAAEHGP SPTAAIDSGK EYDIYNVIVD 300 PQWQEKIRAL EPPVLYWFET RKGRKAWMKK YSPGIPHGSK YRVYFNTANG PLERVPAWAT 360 YVQPEVDGRQ ACAIHWEPSP EQAYKWKNMS PKVPKSLRIY EAHVGISGSE PKISSFNDFT 420 DKVLPYIKEA GYNAIQLIGI VEHKDYFTVG YRVTNFFAVS SRYGTPEDFK RLVDEAHGLG 480 LLIILEIVHS YAAADEMVGL SMFDGSNDCF FRSGKRGQHK FWGTRMFKYG DPDVLHFLLS 540 NLNWWIVEYQ IDGFQFHSVS SMMYTHNGFA SFTGELEEYC NQYVDKDALV YLILANEILH 600 SLHPNIITIA EDATFYPGLC EPTSQGGLGF DYYVNLSVPD MWSTFLESVP DHEWSMTKIV 660 NTLVSNREHA DKMLMYAENH NQSISGRRSF AEILFGEIDE NSNHYKESLL RGSSLHKIIR 720 LITLTIGGRA YLNFMGNEFG HPKRVEFPTS SNNNSYLLAN RQWDLLTKDG VHRDLFAFDK 780 DMMKLDENVK VLSRNIPNIH HVNDSSMVIS YIRGPLLFIF NFHPKDSYDS YSIGVEEAGE 840 YQIILNTDEI KYGGQGILKE EQYFLKTISR RVDGLRNCLE VSLPSRTSQV YKLRRILRI* 900 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02447 | PLN02447 | 1.0e-14 | 92 | 184 | 93 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 1.0e-179 | 380 | 780 | 408 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 3 | 899 | 903 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 0 | 3 | 899 | 909 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 332 | 891 | 570 | + 1,4-alpha-glucan-branching enzyme | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB02827.1 | 0 | 4 | 899 | 3 | 903 | starch-branching enzyme-like protein [Arabidopsis thaliana] |
| EMBL | CBI26672.1 | 0 | 3 | 899 | 2 | 896 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_001154629.1 | 0 | 4 | 899 | 3 | 899 | alpha-amylase/ catalytic/ cation binding [Arabidopsis thaliana] |
| RefSeq | XP_002278858.1 | 0 | 3 | 899 | 2 | 866 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002529457.1 | 0 | 1 | 890 | 1 | 892 | 1,4-alpha-glucan branching enzyme, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 333 | 856 | 114 | 648 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3aml_A | 0 | 333 | 856 | 114 | 648 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 333 | 856 | 114 | 648 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| BF272517 | 278 | 354 | 631 | 0 |
| CO104525 | 273 | 381 | 653 | 0 |
| JG636329 | 270 | 341 | 610 | 0 |
| DY965821 | 293 | 269 | 555 | 0 |
| EH718768 | 263 | 359 | 621 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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