| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma20g03620.2 |
| Family | GH32 |
| Protein Properties | Length: 556 Molecular Weight: 62887.5 Isoelectric Point: 8.4075 |
| Chromosome | Chromosome/Scaffold: 20 Start: 3466871 End: 3471228 |
| Description | cell wall invertase 4 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 39 | 351 | 0 |
| HFQPLKNWMNDPNGPMYYNGVYHLFYQYNPNGTVWGNIVWAHSVSKDLINWNGIEHAIYPSKPFDKFGCWSGSATIIPGKGPVILYTGVIDENNTQVQCY AEPEDPNDPLLRRWVKPDKLNPAVVDKDVNHTEFRDPTTAWWGKDGHWRMLVGSVRKRRGIAYLYRSKDFKTWVRAKHPIHSKGGTGMWECPDFYPVSVI GNVVGNPVKHVLKNSLDDTKFDYYTVGTYLEDKDRYVPDNTSVDGWGGLRYDYGNFYASKSFFDPSKNRRILWGWANECDKPIDNFRKGWAGIQAIPRTV WLDFTGRQLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 556 Download |
| MALPSTKMPV VFYSMVLLII NNCIEAVSVR GDYHRTGFHF QPLKNWMNDP NGPMYYNGVY 60 HLFYQYNPNG TVWGNIVWAH SVSKDLINWN GIEHAIYPSK PFDKFGCWSG SATIIPGKGP 120 VILYTGVIDE NNTQVQCYAE PEDPNDPLLR RWVKPDKLNP AVVDKDVNHT EFRDPTTAWW 180 GKDGHWRMLV GSVRKRRGIA YLYRSKDFKT WVRAKHPIHS KGGTGMWECP DFYPVSVIGN 240 VVGNPVKHVL KNSLDDTKFD YYTVGTYLED KDRYVPDNTS VDGWGGLRYD YGNFYASKSF 300 FDPSKNRRIL WGWANECDKP IDNFRKGWAG IQAIPRTVWL DFTGRQLVQW PVEELNSLRG 360 KEVNIDNQRL EKGDYSEVKG ITAAQADVEV TFSFSSLDKA EAYDPKWVKA QDLCAQKGSK 420 LQGGVGPFGL LTLASQNLEE FTPVFFRVFK SPNKHIVLLC SDARSSSLKS DLYKPQFAGF 480 VDVDLAADKK ISLRSLIDHS VVESFGAGGK TNILSRVYPE LAVMNQAHLF VFNNGTEPIV 540 VQNLKAWSMI SADIK* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 8.0e-64 | 32 | 519 | 508 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| TIGR01322 | scrB_fam | 8.0e-66 | 28 | 519 | 514 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| cd08996 | GH32_B_Fructosidase | 3.0e-94 | 45 | 354 | 321 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 8.0e-145 | 39 | 351 | 322 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 2.0e-179 | 39 | 509 | 481 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABD32689.1 | 0 | 10 | 554 | 11 | 570 | Sialidase [Medicago truncatula] |
| EMBL | CAA49162.1 | 0 | 34 | 549 | 59 | 585 | beta-fructofuranosidase [Daucus carota] |
| EMBL | CAA84526.1 | 0 | 8 | 552 | 8 | 572 | beta-fructofuranosidase; cell wall invertase I; fructosidase [Vicia faba var. minor] |
| Swiss-Prot | P26792 | 0 | 34 | 549 | 59 | 585 | INV1_DAUCA RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 1; AltName: Full=Sucrose hydrolase 1; AltName: Full=Invertase 1; AltName: Full=Cell wall beta-fructosidase 1; Flags: Precursor |
| RefSeq | XP_002530337.1 | 0 | 34 | 549 | 47 | 567 | Beta-fructofuranosidase, insoluble isoenzyme 3 precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 34 | 554 | 8 | 540 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 34 | 554 | 4 | 536 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 34 | 554 | 4 | 536 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 34 | 554 | 4 | 536 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 34 | 554 | 4 | 536 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
