| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma20g33120.2 |
| Family | GT64 |
| Protein Properties | Length: 762 Molecular Weight: 85937.1 Isoelectric Point: 9.3761 |
| Chromosome | Chromosome/Scaffold: 20 Start: 41747236 End: 41753009 |
| Description | glycosyltransferase family protein 47 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT64 | 514 | 746 | 0 |
| FTLLTMTYDARLWNLKMYVKHYSRCSSVREIVVVWNKGVPPKLSDLDSAVPVRIREEKNNSLNNRFRADPLIKTRAVLELDDDIMMPCDDIERGFNVWRQ HPDRIVGFYPRLIDGSLLKYRGEKYARMHKGYNMILTGAAFIDSQVAFKRYGSKEAEKGRELVDRIFNCEDVLLNYLYANASSSSRTVDYVKPAWAIDTS KFSGAAISRNTKVHYQLRSQCLMKFSEMYGSLA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 762 Download |
| MGSGQIVGGG NGGSCCDMSV KCSCRWRLEN QQYYKRLFSS GFVFFFGCFV LFGSIATLYG 60 WLAFSPTVHT SLSSFGCRDD NEGSWSVGVF YGDSPFSLKP IEAANVSNDE SAAWPVANPV 120 VTCASVSDAG YPSNFVADPF LFIQGNTFYL FYETKSSITM QGDIGVSKST DKGATWQQLG 180 IALNEDWHLS YPYVFEHDGQ IYMMPEGSQK GDLRLYRAVN FPLQWRLEKV VMKKPLVDSF 240 VINHGGRYWL FGSDHSGFGT QKNGQLEIWY SNSLLGPWKP HKKNPIYNID KSLGARNGGR 300 PFKYEGNHYR MGQDCGDTYG RKLRIFKIET LTSGEYKEVE VPLGFVESNK GRNAWNGARY 360 HHLDVQHLPS GGWVGVMDGD RVPSGDSVRR FTVGCASVVV AVILIVLLGV LLGFVNCIVP 420 LNWFIHNSGK RNFTALSWER SNMFSSRVRR FCSRLNRAPT FLRGKIKHNA CARRFILAII 480 FAVGVGLMCI GVKNIYGGNG SEEPYPLKGQ YSQFTLLTMT YDARLWNLKM YVKHYSRCSS 540 VREIVVVWNK GVPPKLSDLD SAVPVRIREE KNNSLNNRFR ADPLIKTRAV LELDDDIMMP 600 CDDIERGFNV WRQHPDRIVG FYPRLIDGSL LKYRGEKYAR MHKGYNMILT GAAFIDSQVA 660 FKRYGSKEAE KGRELVDRIF NCEDVLLNYL YANASSSSRT VDYVKPAWAI DTSKFSGAAI 720 SRNTKVHYQL RSQCLMKFSE MYGSLAGRKW GFDSRKDGWD V* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd08772 | GH43_62_32_68 | 0.003 | 142 | 289 | 169 | + Glycosyl hydrolase families: GH43, GH62, GH32, GH68. Members of the glycosyl hydrolase families 32, 43, 62 and 68 (GH32, GH43, GH62, GH68) all possess 5-bladed beta-propeller domains and comprise clans F and J, as classified by the carbohydrate-active enzymes database (CAZY). Clan F consists of families GH43 and GH62. GH43 includes beta-xylosidases, beta-xylanases, alpha-L-arabinases, and alpha-L-arabinofuranosidases, using aryl-glycosides as substrates, while family GH62 contains alpha-L-arabinofuranosidases (EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose sidechains from xylans. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Clan J consists of families GH32 and GH68. GH32 comprises sucrose-6-phosphate hydrolases, invertases, inulinases, levanases, eukaryotic fructosyltransferases, and bacterial fructanotransferases while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. Members of this clan are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) that catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. Structures of all families in the two clans manifest a funnel-shaped active site that comprises two subsites with a single route for access by ligands. | ||
| cd08978 | GH_F | 0.003 | 114 | 287 | 189 | + Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam09258 | Glyco_transf_64 | 1.0e-69 | 514 | 743 | 232 | + Glycosyl transferase family 64 domain. Members of this family catalyze the transfer reaction of N-acetylglucosamine and N-acetylgalactosamine from the respective UDP-sugars to the non-reducing end of [glucuronic acid]beta 1-3[galactose]beta 1-O-naphthalenemethanol, an acceptor substrate analog of the natural common linker of various glycosylaminoglycans. They are also required for the biosynthesis of heparan-sulphate. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0016758 | transferase activity, transferring hexosyl groups |
| GO:0031227 | intrinsic to endoplasmic reticulum membrane |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB85556.1 | 0 | 42 | 761 | 40 | 764 | putative protein [Arabidopsis thaliana] |
| RefSeq | NP_196070.2 | 0 | 42 | 761 | 41 | 765 | glycosyltransferase family protein 47 [Arabidopsis thaliana] |
| RefSeq | XP_002262646.1 | 0 | 66 | 760 | 62 | 755 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002315348.1 | 0 | 14 | 761 | 21 | 847 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532924.1 | 0 | 72 | 761 | 16 | 704 | transferase, transferring glycosyl groups, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1on8_B | 3e-24 | 506 | 745 | 22 | 270 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| PDB | 1on8_A | 3e-24 | 506 | 745 | 22 | 270 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| PDB | 1on6_B | 3e-24 | 506 | 745 | 22 | 270 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| PDB | 1on6_A | 3e-24 | 506 | 745 | 22 | 270 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
| PDB | 1omz_B | 3e-24 | 506 | 745 | 22 | 270 | A Chain A, Crystal Structure Of A Family Gt4 Glycosyltransferase From Bacillus Anthracis Orf Ba1558. |
