| Basic Information | |
|---|---|
| Species | Gossypium raimondii |
| Cazyme ID | Gorai.002G046400.3 |
| Family | GT20 |
| Protein Properties | Length: 936 Molecular Weight: 105470 Isoelectric Point: 7.1646 |
| Chromosome | Chromosome/Scaffold: 02 Start: 3862409 End: 3877738 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 94 | 561 | 0 |
| RQRLLVVANRLPVSAVRRGEDSWSLEISAGGLVTALLGVKEFETRWIGWAGVNVPDEIGQKALTKALAEKWCIPVFLDEEIVHQYYNGYCNNILWPLFHY LGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNMHYEEGDVVWCHDYHLMYLPECLKNHNNNMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVG FHTYDYARHFVSACTRILGIEGTPEGVEDQGRLTRVAAFPIGIDSLRFQNALLVPQVQEHIKELKERFSGRKVMLGVDRLDMIKGIPQKILAFEKFLEEN PNWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGSLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEFVACQEAKKGVLIL SEFAGAAQSLGAGAILVNPWNITEVAASIAQALNMSAEEREKRHQHNFHHVTKHTAQEWAEMFVSELN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 936 Download |
| MPGNKYNGNS PHIPTRTERL LRDRELREQR KSNRTSHLDE VIDIRKGAEV SENGPRFREG 60 DNSRAAFVEK YLEDAAVARA LTEGCERQDG RPFRQRLLVV ANRLPVSAVR RGEDSWSLEI 120 SAGGLVTALL GVKEFETRWI GWAGVNVPDE IGQKALTKAL AEKWCIPVFL DEEIVHQYYN 180 GYCNNILWPL FHYLGLPQED RLATTRSFQS QFAAYKKANQ MFADVVNMHY EEGDVVWCHD 240 YHLMYLPECL KNHNNNMKVG WFLHTPFPSS EIHRTLPSRS ELLRSVLAAD LVGFHTYDYA 300 RHFVSACTRI LGIEGTPEGV EDQGRLTRVA AFPIGIDSLR FQNALLVPQV QEHIKELKER 360 FSGRKVMLGV DRLDMIKGIP QKILAFEKFL EENPNWRDKV VLLQIAVPTR TDVPEYQKLT 420 SQVHEIVGRI NGRFGSLTAV PIHHLDRSLD FHALCALYAV TDVALVTSLR DGMNLVSYEF 480 VACQEAKKGV LILSEFAGAA QSLGAGAILV NPWNITEVAA SIAQALNMSA EEREKRHQHN 540 FHHVTKHTAQ EWAEMFVSEL NDTVVEAQLR TSKEPPELPQ NDAVERYLLS GNRLLILGFN 600 STLTEPVDTS GNRSDQIREK NPNLHPELKE HLTALCNDKK TTIVVLSGSK SEVLDKNFAE 660 YDMWLAAENG MFLRHTKGDW MTTMPEHLNM EWVDSLKHVF EYFTERTPRS EFEIRTTSLV 720 WNYKYADVEF GRLQARDMLQ HLWTGPISNA SVDVVQGSRS VEVRPVGVTK GAAIDRILGE 780 IVHNTSMTTP IDYVLCVGHF LGKDEDVYTF FEPELPSDVN MAKCKPIDAP KLPTEKKAPS 840 KLPATKSGPK SSQTKTPKPS PAPDKRTGNN HSGGSSRRAS PEKVKEKEKM AWSVLDLKGD 900 NYFSCAVGRK RTSARYLLGS SDDVVSFLSR LAKGT* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 935 | 938 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 89 | 932 | 844 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| pfam00982 | Glyco_transf_20 | 0 | 95 | 562 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| TIGR02400 | trehalose_OtsA | 0 | 96 | 562 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 0 | 96 | 778 | 690 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAA69879.1 | 0 | 1 | 935 | 1 | 939 | trehalose-6-phosphate synthase [Arabidopsis thaliana] |
| RefSeq | NP_177979.1 | 0 | 1 | 935 | 1 | 939 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002285632.1 | 0 | 1 | 932 | 1 | 921 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002331300.1 | 0 | 1 | 935 | 1 | 921 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 932 | 1 | 920 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 96 | 564 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 96 | 564 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 96 | 564 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 96 | 564 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 96 | 570 | 3 | 464 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 393 | 457 | 848 | 0 |
| ES848514 | 286 | 379 | 664 | 0 |
| ES812747 | 326 | 451 | 774 | 0 |
| CX546763 | 304 | 83 | 385 | 0 |
| DW069910 | 288 | 182 | 469 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
