Basic Information | |
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Species | Gossypium raimondii |
Cazyme ID | Gorai.004G200800.1 |
Family | GH32 |
Protein Properties | Length: 703 Molecular Weight: 78184.4 Isoelectric Point: 5.14 |
Chromosome | Chromosome/Scaffold: 04 Start: 52679541 End: 52682845 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 138 | 456 | 0 |
HFQPEKNWMNDPNGPLFYNGWYHFFYQYNPNGAVWGDIVWGHAVSKDLINWFHLPLAMVADQWYDKNGVWTGSATILPDGKIVMLYTGSTTEAVQVQNLA FPANHSDPFLIDWVKYHGNPVLVPPPGIYKKDFRDPTTAWLTSEGKWRITIGSKRDKTGISLVYDTKDFINYEMLEGELHAVSGTGMWECVDFFPVSKTE EKGLDTSINGPEVKHVVKVSLDDDRHDYYALGTYHEKNGSWIPDLPEIDVGIGIRYDYGIFYASKTFYDQNKNRRVLWGWIGEGDSEAADMEKGWASLQS IPRTILFDNNTGTNLLQWP |
Full Sequence |
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Protein Sequence Length: 703 Download |
MVDTKPLLPA SNSDGSYLTG SRVNILKPIK VYLTIFCGLL AVGLFAALLI GNYWSDHARI 60 HGSLATSTSK RTHPLRPVSR GPAQGVSEKT SGIWAGVSRG PTQGVSEKTN RVRAGVPWNI 120 LPEPWNSTML EWQRTAFHFQ PEKNWMNDPN GPLFYNGWYH FFYQYNPNGA VWGDIVWGHA 180 VSKDLINWFH LPLAMVADQW YDKNGVWTGS ATILPDGKIV MLYTGSTTEA VQVQNLAFPA 240 NHSDPFLIDW VKYHGNPVLV PPPGIYKKDF RDPTTAWLTS EGKWRITIGS KRDKTGISLV 300 YDTKDFINYE MLEGELHAVS GTGMWECVDF FPVSKTEEKG LDTSINGPEV KHVVKVSLDD 360 DRHDYYALGT YHEKNGSWIP DLPEIDVGIG IRYDYGIFYA SKTFYDQNKN RRVLWGWIGE 420 GDSEAADMEK GWASLQSIPR TILFDNNTGT NLLQWPVEEI ESLRLNSTEF EKVEVKAGSV 480 VPLDVGPGSQ LDIIAEFEMD KEALKKATGS NITFSCQTGG GAAKSGALGP FGILVLADES 540 LSEQTPVYFY IAKDSDGDLK TFFCTDLSRS SKAPDVDKQI FGSYVPVLKD ENLSLRVLVD 600 HSIVESFAQG GRTVITSRIY PTEAINGASR VFLFNNATET CVIASVKIWQ MDSAFIRPYS 660 DDDDKDDDDR KSSAPCLLLC PINYIVSCVT CLYFIYLVSK LS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 6.0e-61 | 131 | 621 | 518 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 6.0e-76 | 134 | 629 | 506 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-95 | 144 | 459 | 323 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-154 | 138 | 456 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 138 | 611 | 484 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB82419.1 | 0 | 102 | 665 | 90 | 650 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 102 | 665 | 90 | 650 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35859.1 | 0 | 97 | 697 | 89 | 675 | soluble acid invertase [Pyrus pyrifolia var. culta] |
RefSeq | XP_002303519.1 | 0 | 95 | 662 | 95 | 660 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532576.1 | 0 | 97 | 661 | 85 | 647 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 117 | 659 | 2 | 544 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
PDB | 3ugh_A | 0 | 117 | 659 | 2 | 544 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
PDB | 3ugg_B | 0 | 117 | 659 | 2 | 544 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
PDB | 3ugg_A | 0 | 117 | 659 | 2 | 544 | A Chain A, Raphanus Sativus Anionic Peroxidase. |
PDB | 3ugf_B | 0 | 117 | 659 | 2 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |